| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,705,327 | A→G | L14P (CTC→CCC) | yhjX ← | MFS transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,705,327 | 0 | A | G | 94.5% | 172.3 / 1.7 | 55 | L14P (CTC→CCC) | yhjX | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (29/23); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
CCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGAAATGCCAT > NZ_CP009273/3705194‑3705462 | ccccAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTc < 1:934520/139‑1 (MQ=255) aGACTTAACAAGCCGAAAGAGAAAGCGCCCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTcagcc < 1:356638/139‑1 (MQ=255) aaCAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTACACAGGCTCCAGGTATAAACCGACCCCAGCGCAACCTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCggg > 1:370047/1‑139 (MQ=255) aaCAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCggg > 1:979950/1‑139 (MQ=255) aaCAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCggg > 1:1282672/1‑139 (MQ=255) aCAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGt < 2:105422/139‑1 (MQ=255) ccGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCt > 1:338181/1‑139 (MQ=255) gAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGa < 1:1039729/139‑1 (MQ=255) aaaGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGAt < 2:979950/139‑1 (MQ=255) aaaGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAAttt > 2:1281959/1‑138 (MQ=255) aaGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTg > 2:954393/1‑138 (MQ=255) aGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGaa < 2:950713/139‑1 (MQ=255) gCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAg < 1:215361/139‑1 (MQ=255) cGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAgg < 2:855992/139‑1 (MQ=255) cGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAgg < 2:657989/139‑1 (MQ=255) gCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCa < 1:696433/137‑1 (MQ=255) cGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATaaa > 1:607510/1‑138 (MQ=255) cgcCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAAt > 2:382130/1‑139 (MQ=255) cAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc < 2:966441/139‑1 (MQ=255) cAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc < 1:450472/139‑1 (MQ=255) cAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc < 2:367567/139‑1 (MQ=255) cAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc < 1:929024/139‑1 (MQ=255) ggCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACaa < 1:25764/139‑1 (MQ=255) tCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCa > 1:84965/1‑139 (MQ=255) aGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATaa > 1:1023684/1‑139 (MQ=255) gTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACg < 1:547308/139‑1 (MQ=255) taAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTAt < 1:1231913/91‑1 (MQ=255) taAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTAt > 2:1231913/1‑91 (MQ=255) taAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAg > 2:636484/1‑139 (MQ=255) aaCCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg > 1:112981/1‑139 (MQ=255) aaCCGACCCCAGCGCAAACTGGGTAATGATAGTA‑CGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTg < 2:798436/138‑1 (MQ=255) aaCCGACACCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGTCCACCATAACGAAGt < 2:1280734/138‑1 (MQ=255) aCCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGc < 2:1189121/139‑1 (MQ=255) aCCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAAtct < 2:1205230/111‑1 (MQ=255) aCCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAAtct > 1:1205230/1‑111 (MQ=255) aCCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACtt > 1:484654/1‑139 (MQ=255) aCCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACtt > 2:248380/1‑139 (MQ=255) aCCCCAGCGCAAACTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACtt > 2:280894/1‑139 (MQ=255) aCTGGGTAATGATAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTgggg > 1:37488/1‑138 (MQ=255) tAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc < 2:846790/95‑1 (MQ=255) tAGTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGAc > 1:846790/1‑95 (MQ=255) gTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTg > 2:541375/1‑88 (MQ=255) gTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTg < 1:541375/88‑1 (MQ=255) gTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGaaa > 2:1040047/1‑139 (MQ=255) gTACCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGaaa > 1:156414/1‑139 (MQ=255) gTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGaaa > 1:306962/1‑139 (MQ=255) aCCGATGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGAAATg > 2:1016810/1‑139 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGgtgt > 2:573916/1‑41 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGgtgt < 1:573916/41‑1 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGc < 2:830422/47‑1 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGc > 1:830422/1‑47 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGAAATGCCAt > 2:430075/1‑139 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGAAATGCCAt > 2:548220/1‑139 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGAAATGCCAt > 1:1101854/1‑139 (MQ=255) aTGGGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGAAATGCCAt > 1:68785/1‑139 (MQ=255) | CCCCAGACTTAACAAGCCGAAAGAGAAAGCGACCTGGCTTACCGGCGCATCCAGCTTGGCGGAAAGCGCGCCATTAAACAGGCTCCAGGTATAAACCGACCCCAGCGCAAACTGGGTAATGATAGTACCGATGAGTGTCAGCCAGCGGGTACGCTGATAATTTGAAGGTGTCATGGCAGTATTCCTGCAGTAATAAAAAGGAAAAATCTCTGCCGACAACCATAACGAAGTGCACTTTATGCCTGGGGAAAAACGGCATGAAATGCCAT > NZ_CP009273/3705194‑3705462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |