Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,710,430	C→T	A175V (GCG→GTG)	yiaD →	OmpA family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,710,430	0	C	T	93.3%	248.0 / NA	78	A175V (GCG→GTG)	yiaD	OmpA family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  major base T (31/39);  minor base G (5/0);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 2.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGAAATTACCTTAAGCCCGCT  >  NZ_CP009273/3710296‑3710562                                                                                                                                       |                                                                                                                                     ccTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTg                                                                                                                                  <  1:970746/139‑1 (MQ=255) ccTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGGCACGACCTGAAAATGCGGCTCTCCCAGCAACGTGCGGGTTCCGTTGCCCGCGTGTTg                                                                                                                                  >  2:1180098/1‑139 (MQ=255) ccTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGGTTTACCGACAGCACGGGGGGGCACGACCTGAACATGCGTCTCTCCCACCAACCCGCGGATTCCGTTGCCAGCGGGTTg                                                                                                                                  >  2:232493/1‑139 (MQ=255) ccTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAAAGGGATTGGGTTTACCGACAGGACGGGGGGGTACGGCCTGAACAAGCGTCTCTCCCAGCAACGTGCGGGTTCCGTTGCCAGCGTGtt                                                                                                                                   >  1:311430/1‑138 (MQ=255)  cTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGa                                                                                                                                 <  2:262534/139‑1 (MQ=255)  cTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGa                                                                                                                                 <  1:1180098/139‑1 (MQ=255)  cTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGa                                                                                                                                 <  2:671803/139‑1 (MQ=255)   tGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGGTATACCGACAGCACGGGGGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGAt                                                                                                                                >  1:597969/1‑139 (MQ=255)     aCCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGAt                                                                                                                                <  1:1119565/137‑1 (MQ=255)     aCCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGAt                                                                                                                                <  1:627137/137‑1 (MQ=255)     aCCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATc                                                                                                                               <  2:740827/138‑1 (MQ=255)     aCCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCa                                                                                                                              >  1:116689/1‑139 (MQ=255)     aCCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAAAGTGATTTGGTATACCGACAGCACCGGGGGTCACGACCTGAAAAAGCGTTTCTCCCAGCAAAGGGCGGGTTCCCGTGCCAGCGGGGTGATCa                                                                                                                              >  2:530260/1‑139 (MQ=255)         gCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGGTTGGGTATACCCACAGCACGGGGGGTCACGACCTGAACATACGTCTCTCCCAGCAACGGGCGGGTTTCGTTTCCAGCGTGGTGAtttgtct                                                                                                                          >  2:151893/1‑133 (MQ=255)          cGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGGCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAg                                                                                                                         >  1:110361/1‑139 (MQ=255)          cGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACAGGGTTTGGTATACCGACAGCAAGGGGGGGCACGACCTTAACAATCGGCTCCCCCCGCCACGTGGGGGGTTCGGTGGCAGCGTGTTGATCcccc                                                                                                                           >  2:1262167/1‑137 (MQ=255)                    gTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTTGGTATACCGACAACACCGGTGGGCCCGACCTGAAAATGCGGCTCTCCCCGCAACGTGCGGGTTTCGTTGCCAGCGGGTTTGTCACCCCGGGGGTGGACg                                                                                                               >  2:1042126/1‑139 (MQ=255)                           aaGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCTAGCCg                                                                                                        <  1:109967/139‑1 (MQ=255)                           aaGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        <  1:963185/139‑1 (MQ=255)                           aaGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        <  2:1001569/139‑1 (MQ=255)                           aaGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        <  1:530260/139‑1 (MQ=255)                           aaGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        <  1:264674/139‑1 (MQ=255)                           aaGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        <  2:1049827/139‑1 (MQ=255)                               gTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        <  2:243940/135‑1 (MQ=255)                               gTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAAAAAGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        >  1:243940/1‑135 (MQ=255)                                tATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATcc                                                                                                   <  1:1042126/139‑1 (MQ=255)                                tATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATcc                                                                                                   <  1:763522/139‑1 (MQ=255)                                tATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATcc                                                                                                   <  2:887618/139‑1 (MQ=255)                                 aTCCGAAAACGGCGGTTAACGTGATTGGGTATACCGACAGCACGGGTGGGCACGACCTGAAAATGCGTCTCTCCCAGCAAAGTGCGGATTCCGTTGCCAGCGGGTTGATCACCCAGGGCGTGGACGCCAGCCGCATccc                                                                                                  >  1:756564/1‑138 (MQ=255)                                  tCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCg                                                                                                  <  1:793559/138‑1 (MQ=255)                                  tCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCg                                                                                                  <  1:232493/138‑1 (MQ=255)                                  cccGAAAGCGGCGGTTAACGTGATTGGTTATACCGACAGCGCGGGTGGTCTCGCCCTGAATATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCGGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATcc                                                                                                   <  2:938137/136‑1 (MQ=255)                                    cGAAAACGGCGGTTAACGTGGTTGGTTATACCGGCAGCACGGGGGGTCACGACCTGAAAAAGCATCTCTCCCAGCAACATGCGGATTCCGTTGGCAGCGCGTTGATCACCCAGGGCGTGGAAGCCAGACGCAAACGTAc                                                                                               >  1:1049827/1‑139 (MQ=255)                                     gcaaaCGGCGGTTAACGTGATTGGTTTTACCGACAGCACGGGGGGTTACGCCCTGAACCCGCGTGTCTCCCAGCAACGTGCGGAACTCGTTGCCAGCGGCTTGATCACCCAGGGGGAGGTCCCCAGCAGCACCTGTAcc                                                                                              >  2:595957/3‑138 (MQ=255)                                      aaaaCGGCGGTTAACGTGATTGGTTATACCGACAGTACGGGTGGTCACGGCCTGAACATGCGTCTCTCCCAGCAACTTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTc                                                                                             <  2:1247282/139‑1 (MQ=255)                                                  aaCGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGc                                                                                  <  1:1066338/138‑1 (MQ=255)                                                  aaCGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGc                                                                                  <  2:203363/138‑1 (MQ=255)                                                               ataCCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCa                                                                                                                          <  2:1038395/85‑1 (MQ=255)                                                               ataCCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCa                                                                                                                          >  1:1038395/1‑85 (MQ=255)                                                               ataCCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAAt                                                                    >  2:950134/1‑139 (MQ=255)                                                                   cGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGcc                                                                <  2:413945/139‑1 (MQ=255)                                                                     aCAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGa                                                                                                                                 >  2:745699/1‑72 (MQ=255)                                                                     aCAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGa                                                                                                                                 <  1:745699/72‑1 (MQ=255)                                                                      cAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAg                                                              >  1:119597/1‑138 (MQ=255)                                                                      cAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAg                                                              >  2:916056/1‑138 (MQ=255)                                                                       aGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGa                                                                                                                                 >  2:863836/1‑70 (MQ=255)                                                                       aGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGa                                                                                                                                 <  1:863836/70‑1 (MQ=255)                                                                         cACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAAc                                                          <  2:116689/139‑1 (MQ=255)                                                                                 gtCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCg                                                   >  1:588640/1‑138 (MQ=255)                                                                                 gtCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCg                                                   >  2:380274/1‑138 (MQ=255)                                                                                        ccTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAgg                                            >  2:261684/1‑138 (MQ=255)                                                                                            aaCATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAgg                                       >  2:304255/1‑139 (MQ=255)                                                                                                tGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGgcgc                                    >  2:534538/1‑138 (MQ=255)                                                                                                 gCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGcc                                                                >  2:720348/1‑109 (MQ=255)                                                                                                 gCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGcc                                                                <  1:720348/109‑1 (MQ=255)                                                                                                  cGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCAcc                                                                                                                            >  1:186999/1‑48 (MQ=255)                                                                                                  cGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCAcc                                                                                                                            <  2:186999/48‑1 (MQ=255)                                                                                                      tctcCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACg                                                                                                               <  1:1204411/57‑1 (MQ=255)                                                                                                      tctcCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACg                                                                                                               >  2:1204411/1‑57 (MQ=255)                                                                                                      tctcCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAAccg                             >  1:1108318/1‑139 (MQ=255)                                                                                                      tctcCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAAccg                             >  2:107468/1‑139 (MQ=255)                                                                                                        tcCCGGCAACGTGCGGATTCCGTTCCCGGCGTGTTGATCACCGAGGTGGTGGCCGCCATCCGCATCCGTGCTCAGGGCCTTGGCCCGGCTACCCCAGTCTCCAGAAACGGCACCGCAGAAGGTAAGGCGCAAAAccg                             <  2:909279/137‑1 (MQ=255)                                                                                                        tcCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAAccg                             <  2:588640/137‑1 (MQ=255)                                                                                                        tcCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAAccg                             <  1:719882/137‑1 (MQ=255)                                                                                                        tcCCAGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAAccg                             <  2:110361/137‑1 (MQ=255)                                                                                                            aGCAACGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCgtg                        >  1:185366/1‑138 (MQ=255)                                                                                                                 cGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCg                                                                  <  1:1190225/91‑1 (MQ=255)                                                                                                                 cGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCg                                                                  >  2:1190225/1‑91 (MQ=255)                                                                                                                 cGTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGaaa                  >  2:675455/1‑139 (MQ=255)                                                                                                                  gTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        <  2:665691/52‑1 (MQ=255)                                                                                                                  gTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCg                                                                                                        >  1:665691/1‑52 (MQ=255)                                                                                                                  gTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGc                                                                                  >  2:79094/1‑74 (MQ=255)                                                                                                                  gTGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGc                                                                                  <  1:79094/74‑1 (MQ=255)                                                                                                                  gTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGTCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGAAAt                 >  1:913577/1‑139 (MQ=255)                                                                                                                   tGCGGATTCCGTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGAAAt                 >  1:901535/1‑138 (MQ=255)                                                                                                                             gTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGAAATTACCTTAAGcc      <  2:333127/139‑1 (MQ=255)                                                                                                                             gTTGCCAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGAAATTACCTTAAGcc      >  1:827059/1‑139 (MQ=255)                                                                                                                                 ccAGCGTGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGAAATTACCTTAAGCCCGCt  <  2:119597/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAGGTAAGGCGCAAAACCGCCGTGTAGAAATTACCTTAAGCCCGCT  >  NZ_CP009273/3710296‑3710562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑