Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,750,235 T→C E318G (GAA→GGA)  yiaY ← L‑threonine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,750,2350TC96.7% 301.3 / 1.6 90E318G (GAA→GGA) yiaYL‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (33/54);  total (34/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

CCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTTCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGG  >  NZ_CP009273/3750100‑3750366
                                                                                                                                       |                                                                                                                                   
ccACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcg                                                                                                                                  >  1:395897/1‑139 (MQ=255)
  acaGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcgcg                                                                                                                                <  1:510070/139‑1 (MQ=255)
  acaGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCcgcg                                                                                                                                <  1:767936/139‑1 (MQ=255)
    aGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGcgtc                                                                                                                              >  1:567609/1‑139 (MQ=255)
    aGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGcgtc                                                                                                                              >  1:945732/1‑139 (MQ=255)
    aGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGcgtc                                                                                                                              >  2:1088958/1‑139 (MQ=255)
             tCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTAc                                                                                                                      <  1:147438/138‑1 (MQ=255)
              cAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCt                                                                                                                    >  1:1160607/1‑139 (MQ=255)
                 ggCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTc                                                                                                                 <  1:64195/139‑1 (MQ=255)
                 ggCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTc                                                                                                                 <  1:165486/139‑1 (MQ=255)
                 ggCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTc                                                                                                                 <  2:1192031/139‑1 (MQ=255)
                 ggCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTc                                                                                                                 <  1:245460/139‑1 (MQ=255)
                    aTTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACg                                                                                                              <  2:567609/139‑1 (MQ=255)
                         tCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCAc                                                                                                         <  2:806411/139‑1 (MQ=255)
                             cAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGcc                                                                                                      >  2:1192418/1‑138 (MQ=255)
                               aTACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCAt                                                                                                   <  2:1031649/139‑1 (MQ=255)
                                    gcgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAg                                                                                              <  2:1160607/139‑1 (MQ=255)
                                     cgAACTCTTCTCCTTTCACTTACAAGTCGGGTAGGCATTCCGGGCTCTCCACTTTCTTCGCGTGTTCATGGATTGCTCTAATGCATGCTTTCGCCCCTCCCGCGTCGCTTTTACCTGTCACGTTCACGCTCATTGCacc                                                                                             >  2:663974/1‑137 (MQ=255)
                                     cgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGc                                                                                             >  1:1000381/1‑139 (MQ=255)
                                     cgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGc                                                                                             >  1:1252680/1‑139 (MQ=255)
                                     cgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGc                                                                                             >  2:260047/1‑139 (MQ=255)
                                     cgAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGc                                                                                             >  2:811215/1‑139 (MQ=255)
                                         aTCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGca                                                                                         <  1:631239/139‑1 (MQ=255)
                                            ttcttcttTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAg                                                                                      >  2:1143018/1‑139 (MQ=255)
                                              cttcttTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGcac                                                                                        <  1:1088958/135‑1 (MQ=255)
                                                    tCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTTCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  1:611801/136‑1 (MQ=255)
                                                    tCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  2:527797/136‑1 (MQ=255)
                                                    tCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  2:822378/136‑1 (MQ=255)
                                                    tCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  2:1192179/136‑1 (MQ=255)
                                                    tCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 >  1:898121/1‑136 (MQ=255)
                                                           cAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTg                                                                         >  1:394051/1‑137 (MQ=255)
                                                           cAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTg                                                                         >  2:835886/1‑137 (MQ=255)
                                                                  cgTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 >  1:687678/1‑122 (MQ=255)
                                                                  cgTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  2:687678/122‑1 (MQ=255)
                                                                  cgTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGa                                                                >  2:164003/1‑139 (MQ=255)
                                                                       gCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTg                                                           <  2:1252680/139‑1 (MQ=255)
                                                                        ccTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGc                                                          >  1:716191/1‑139 (MQ=255)
                                                                          tGCCGGGATATCCACTTTCTTCGCCAGTTTACGGATTGCGTTAATGCAGGGTTCAGCACCTCCCGCGTCGTTTTTACCTTTCACGTTTCCGTCCATTGCTGCGGCACAGTCACGCGGTCGTGCCGCGGCGACTTTGCTg                                                        >  2:220453/1‑139 (MQ=255)
                                                                           gCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGt                                                       >  2:818352/1‑139 (MQ=255)
                                                                            ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACt                                                              >  1:246891/1‑131 (MQ=255)
                                                                            ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACt                                                              <  2:246891/131‑1 (MQ=255)
                                                                            ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGtt                                                      >  2:320234/1‑139 (MQ=255)
                                                                            ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGtt                                                      >  2:175264/1‑139 (MQ=255)
                                                                            ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGtt                                                      <  1:320234/139‑1 (MQ=255)
                                                                            ccGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGtt                                                      <  2:201722/139‑1 (MQ=255)
                                                                              gggATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGa                                                    <  1:1192418/139‑1 (MQ=255)
                                                                                 atatCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATa                                                 >  2:846590/1‑139 (MQ=255)
                                                                                              tcGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  2:226179/94‑1 (MQ=255)
                                                                                              tcGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 >  1:226179/1‑94 (MQ=255)
                                                                                              tcGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAg                                                                              >  1:705808/1‑97 (MQ=255)
                                                                                              tcGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAg                                                                              <  2:705808/97‑1 (MQ=255)
                                                                                               cGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 >  1:768637/1‑93 (MQ=255)
                                                                                               cGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACg                                                                                 <  2:768637/93‑1 (MQ=255)
                                                                                                     ttCACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCaa                              >  2:915015/1‑138 (MQ=255)
                                                                                                       cACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGca                                                                                         <  2:971127/77‑1 (MQ=255)
                                                                                                       cACGGATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGca                                                                                         >  1:971127/1‑77 (MQ=255)
                                                                                                          ggATGGCGTTAATGCCGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGGCACGTTCACGCCCATTGCATCGTCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTTAACGTGCGGCAGCAAAACGGc                        <  2:298864/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:56189/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:1157841/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:688163/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:182349/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:835886/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:846590/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:863321/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:819041/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:360273/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:1008581/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:210800/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  1:81793/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:153098/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:154271/139‑1 (MQ=255)
                                                                                                          ggATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCAGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:189615/139‑1 (MQ=255)
                                                                                                           gATGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCg                       <  2:395897/139‑1 (MQ=255)
                                                                                                            aTGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGt                      <  2:486115/139‑1 (MQ=255)
                                                                                                            aTGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGt                      <  2:1245212/139‑1 (MQ=255)
                                                                                                             tGGCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGtt                     <  1:260047/139‑1 (MQ=255)
                                                                                                               gCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTtac                   <  2:378966/139‑1 (MQ=255)
                                                                                                               gCGTTAATGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTta                    >  1:449684/1‑138 (MQ=255)
                                                                                                                    aaTGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTtacataca              >  2:1272460/1‑139 (MQ=255)
                                                                                                                     aTGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTtacataca              <  2:716191/138‑1 (MQ=255)
                                                                                                                     aTGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTtacataca              <  1:818352/138‑1 (MQ=255)
                                                                                                                      tGCAGGCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACAcc            <  2:976573/139‑1 (MQ=255)
                                                                                                                          ggCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCgtgt        >  1:114070/1‑139 (MQ=255)
                                                                                                                          ggCTTCAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCgtgt        >  1:1212477/1‑139 (MQ=255)
                                                                                                                               cAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGc     <  2:449684/137‑1 (MQ=255)
                                                                                                                               cAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGc     <  1:1279048/137‑1 (MQ=255)
                                                                                                                               cAGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGc     <  2:898121/137‑1 (MQ=255)
                                                                                                                                aGCACCTTCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        <  2:717486/117‑1 (MQ=255)
                                                                                                                                aGCACCTTCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGc                        >  1:717486/1‑117 (MQ=255)
                                                                                                                                aGCACCTCCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAgg  <  1:1176394/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CCACAGGCATCTTTCAGGGCATTAGTCGCCAATACCGCGAAATCTTCTTCTTTCACGTTCAGGTCGCGTAGGCCTGCCGGGATATCCACTTTCTTCGCCAGTTCACGGATGGCGTTAATGCAGGCTTCAGCACCTTCCGCGTCGTTTTTACCTGTCACGTTCACGCCCATTGCAGCGGCACAGTCACGCAGACGTGCAGCGGCGACTTTGCTGTTGAATACCTGAACGTGCGGCAGCAAAACGGCGTTACATACACCGTGTGGCAGG  >  NZ_CP009273/3750100‑3750366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑