Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	307,835	C→T	P5S (CCG→TCG)	BW25113_RS01515 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	307,835	0	C	T	100.0%	309.7 / NA	76	P5S (CCG→TCG)	BW25113_RS01515	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/42);  total (34/42)

ACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGC  >  NZ_CP009273/307702‑307965                                                                                                                                      |                                                                                                                                   aCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCaa                                                                                                                               <  2:703777/139‑1 (MQ=255) aCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCaa                                                                                                                               <  1:186042/139‑1 (MQ=255) aCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCaa                                                                                                                               <  1:242557/139‑1 (MQ=255) aCTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCaa                                                                                                                               <  2:770590/139‑1 (MQ=255)  cTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCa                                                                                                                                >  1:517062/1‑137 (MQ=255)  cTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCa                                                                                                                                >  2:86573/1‑137 (MQ=255)   tGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCaa                                                                                                                               <  2:517062/137‑1 (MQ=255)      cACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGgga                                                                                                                          <  1:1193251/139‑1 (MQ=255)      cACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCCCAAAgg                                                                                                                            >  1:1122313/1‑137 (MQ=255)           gAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGgaga                                                                                                                     <  2:183396/139‑1 (MQ=255)            aaCGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGAc                                                                                                                    <  1:507404/139‑1 (MQ=255)                tCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGgga                                                                                                                          >  2:959608/1‑129 (MQ=255)                tCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGgga                                                                                                                          <  1:959608/129‑1 (MQ=255)                   gCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAGAGGGAGGAGACTGAGTgg                                                                                                             <  1:567988/139‑1 (MQ=255)                         gggTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       <  2:185795/139‑1 (MQ=255)                         gggTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       <  2:77266/139‑1 (MQ=255)                         gggTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       <  1:278709/139‑1 (MQ=255)                         gggTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       <  2:1028086/139‑1 (MQ=255)                                    ggATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTaa                                                                                               >  1:739674/1‑136 (MQ=255)                                            cTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGa                                                                                    <  1:575094/139‑1 (MQ=255)                                               ttCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGc                                                                                 <  2:764494/139‑1 (MQ=255)                                               ttCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGc                                                                                 <  2:1124172/139‑1 (MQ=255)                                                tCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAaga                                                                                           >  2:1021386/1‑128 (MQ=255)                                                tCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAaga                                                                                           <  1:1021386/128‑1 (MQ=255)                                                 ccTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       <  2:117200/115‑1 (MQ=255)                                                 ccTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       >  1:117200/1‑115 (MQ=255)                                                     tgGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCa                                                                            >  1:468195/1‑138 (MQ=255)                                                     tgGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCa                                                                            >  1:770585/1‑138 (MQ=255)                                                       gCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATAc                                                                         <  2:1097097/139‑1 (MQ=255)                                                         gTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAg                                                                       >  2:311667/1‑139 (MQ=255)                                                         gTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAg                                                                       >  2:189925/1‑139 (MQ=255)                                                          tAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAgg                                                                      >  1:1043166/1‑139 (MQ=255)                                                            aaCGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAgggg                                                                    <  2:87942/139‑1 (MQ=255)                                                               gTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCCCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGa                                                                 <  2:1122313/139‑1 (MQ=255)                                                                   ttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGc                                                              <  1:1277924/138‑1 (MQ=255)                                                                         tAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTca                                                        >  2:287984/1‑138 (MQ=255)                                                                              ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGt                                                  <  2:930471/139‑1 (MQ=255)                                                                               cAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                 <  2:391982/139‑1 (MQ=255)                                                                               cAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                 <  1:189925/139‑1 (MQ=255)                                                                               cAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                 <  1:572686/139‑1 (MQ=255)                                                                                    gATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACAt                                            <  1:287984/139‑1 (MQ=255)                                                                                     aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGc                                                                                                            >  2:447804/1‑74 (MQ=255)                                                                                     aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGc                                                                                                            <  1:447804/74‑1 (MQ=255)                                                                                         aCCACGTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATATAGGGGCGCAGCTGGGCAACAAGTCAACAtt                                           >  1:625406/1‑135 (MQ=255)                                                                                         aCCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACAtt                                           >  2:1188693/1‑135 (MQ=255)                                                                                           cacaTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTcaa                                                       >  2:428084/1‑121 (MQ=255)                                                                                           cacaTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTcaa                                                       <  1:428084/121‑1 (MQ=255)                                                                                              aTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATg                                  >  2:325271/1‑139 (MQ=255)                                                                                                       cTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCaa                                                                                                                               >  1:240032/1‑37 (MQ=255)                                                                                                       cTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCaa                                                                                                                               <  2:240032/37‑1 (MQ=255)                                                                                                       cTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGc                                                                                                            >  1:1027693/1‑56 (MQ=255)                                                                                                       cTTAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGc                                                                                                            <  2:1027693/56‑1 (MQ=255)                                                                                                        ttAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCgg                        >  2:878121/1‑139 (MQ=255)                                                                                                        ttAACAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGGCCAACTTAAGATAATGGAATATTCgg                        >  2:636574/1‑139 (MQ=255)                                                                                                          aaCAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       >  1:443758/1‑58 (MQ=255)                                                                                                          aaCAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       <  2:443758/58‑1 (MQ=255)                                                                                                          aaCAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCg                         >  1:426829/1‑136 (MQ=255)                                                                                                          aaCAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGGATACAGGGGCGAAGCTGGTCAACAAGGCCACATTAAGATAATGGAATATTCg                         >  2:527949/1‑136 (MQ=255)                                                                                                            cAGTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagag                    <  1:918709/139‑1 (MQ=255)                                                                                                              gTTTGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagaacg                  >  1:244931/1‑136 (MQ=255)                                                                                                               tttGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGaa                  >  1:447581/1‑138 (MQ=255)                                                                                                                 tGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATg                                                                                  >  1:539978/1‑72 (MQ=255)                                                                                                                 tGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATg                                                                                  <  2:539978/72‑1 (MQ=255)                                                                                                                 tGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATa                                                                          <  1:833617/80‑1 (MQ=255)                                                                                                                 tGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATa                                                                          >  2:833617/1‑80 (MQ=255)                                                                                                                 tGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACg               >  2:719890/1‑139 (MQ=255)                                                                                                                 tGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATAATCGGAGAGAAACg               >  1:1240581/1‑139 (MQ=255)                                                                                                                 tGAGTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACg               >  2:948529/1‑139 (MQ=255)                                                                                                                    gTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       <  2:300816/48‑1 (MQ=255)                                                                                                                    gTGAAAATGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGtt                                                                                                       >  1:300816/1‑48 (MQ=255)                                                                                                                         aaTGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAg       <  1:311667/139‑1 (MQ=255)                                                                                                                         aaTGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAg       <  1:325271/139‑1 (MQ=255)                                                                                                                          aTGCAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGt      <  1:717475/139‑1 (MQ=255)                                                                                                                             cAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTaaaa                                                                                             >  1:995542/1‑49 (MQ=255)                                                                                                                             cAGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTaaaa                                                                                             <  2:995542/49‑1 (MQ=255)                                                                                                                              aGGCTGCGTCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc  <  2:1240581/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   ACTGGCACTCGAACGTCGGCGACGGGGTTACCGGTGGATCAGACTGTTCCTGTGGCGTAAACGTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGC  >  NZ_CP009273/307702‑307965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑