Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,816,198	C→T	L147L (CTG→TTG)	spoT →	bifunctional GTP diphosphokinase/guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,816,198	0	C	T	100.0%	351.7 / NA	91	L147L (CTG→TTG)	spoT	bifunctional GTP diphosphokinase/guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/50);  total (41/50)

AAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTG  >  NZ_CP009273/3816063‑3816329                                                                                                                                        |                                                                                                                                    aaaCTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTgg                                                                                                                                  <  1:1188290/139‑1 (MQ=255)  aaCTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTggg                                                                                                                                 <  2:595652/139‑1 (MQ=255)        aTAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCAc                                                                                                                            <  2:460791/138‑1 (MQ=255)        aTAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACt                                                                                                                           >  2:719783/1‑139 (MQ=255)             cTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGcc                                                                                                                      <  1:78389/139‑1 (MQ=255)                      cgcgATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAAc                                                                                                             >  1:318635/1‑139 (MQ=255)                        cgATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGt                                                                                                           >  1:1263515/1‑139 (MQ=255)                                     gcgcAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGccc                                                                                              >  2:311777/1‑139 (MQ=255)                                     gcgcAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGccc                                                                                              >  1:54135/1‑139 (MQ=255)                                         aGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGcc                                                                                                                      >  2:313371/1‑111 (MQ=255)                                         aGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGcc                                                                                                                      <  1:313371/111‑1 (MQ=255)                                         aGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACg                                                                                                            <  2:395564/121‑1 (MQ=255)                                         aGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACg                                                                                                            >  1:395564/1‑121 (MQ=255)                                         aGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTg                                                                                           >  2:761340/1‑138 (MQ=255)                                          ggCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGa                                                                                          >  1:56508/1‑138 (MQ=255)                                                 aaCTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCg                                                                                  <  2:1263515/139‑1 (MQ=255)                                                  aCTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGa                                                                                 <  2:729750/139‑1 (MQ=255)                                                   cTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGaa                                                                                <  1:1245223/139‑1 (MQ=255)                                                           aGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAg                                                                        <  1:1052320/139‑1 (MQ=255)                                                           aGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAg                                                                        <  2:369845/139‑1 (MQ=255)                                                             atgatTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATc                                                                                                  >  2:211196/1‑111 (MQ=255)                                                             atgatTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATc                                                                                                  <  1:211196/111‑1 (MQ=255)                                                             atgatTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGcc                                                                      <  2:420431/139‑1 (MQ=255)                                                                atTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCg                                                                    <  2:1248894/138‑1 (MQ=255)                                                                     ggCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  2:317183/139‑1 (MQ=255)                                                                      gCGATGGTGCAGGATCTCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  1:672703/138‑1 (MQ=255)                                                                      gCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcgc                                                             <  2:56508/139‑1 (MQ=255)                                                                      gCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  1:1224804/138‑1 (MQ=255)                                                                      gCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  2:877565/138‑1 (MQ=255)                                                                      gCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  1:761340/138‑1 (MQ=255)                                                                      gCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  2:578199/138‑1 (MQ=255)                                                                       cGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  2:475566/139‑1 (MQ=255)                                                                       cGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  2:1251437/139‑1 (MQ=255)                                                                       cGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  2:689644/139‑1 (MQ=255)                                                                         aTGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCAcc                                                          >  2:829046/1‑139 (MQ=255)                                                                         aTGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCAcc                                                          >  1:89115/1‑139 (MQ=255)                                                                          tGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCg                                                         >  1:1250887/1‑139 (MQ=255)                                                                           ggTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCCATTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGt                                                        >  2:557565/1‑139 (MQ=255)                                                                               cAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAg                                                    <  1:231051/139‑1 (MQ=255)                                                                               cAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAg                                                    <  2:89115/139‑1 (MQ=255)                                                                                    tatCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATc                                               >  1:452783/1‑139 (MQ=255)                                                                                      tCCGCATCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGCAATTTATAGCCCGCTGGCGCACCGTTTAGGTATcca                                             >  2:1125328/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGc                                                                   <  1:737678/116‑1 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGc                                                                   >  2:737678/1‑116 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  2:948496/121‑1 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              >  1:948496/1‑121 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  2:41396/123‑1 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            >  1:41396/1‑123 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  2:166768/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:207018/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  2:846798/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:170866/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:747831/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:704172/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            >  1:436383/1‑139 (MQ=255)                                                                                       ccGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCAGCTGGCGCACCGTTTAGGTATccac                                            >  1:636686/1‑139 (MQ=255)                                                                                        cgcgTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGTCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            <  2:1098060/138‑1 (MQ=255)                                                                                        cgcgTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            <  1:269293/138‑1 (MQ=255)                                                                                        cgcgTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccac                                            <  2:54135/138‑1 (MQ=255)                                                                                           gTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACcaca                                        >  2:1039273/1‑139 (MQ=255)                                                                                            tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            >  2:1260347/1‑118 (MQ=255)                                                                                            tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  1:1260347/118‑1 (MQ=255)                                                                                            tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAt                                       >  2:351654/1‑139 (MQ=255)                                                                                            tCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAt                                       >  2:388704/1‑139 (MQ=255)                                                                                             cATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAt                                       >  1:882331/1‑138 (MQ=255)                                                                                                    atcaAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAAcc                                >  2:315806/1‑138 (MQ=255)                                                                                                    atcaAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAAcc                                >  1:515143/1‑138 (MQ=255)                                                                                                    atcaAACTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCg                               <  2:1250887/139‑1 (MQ=255)                                                                                                          cTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCg                         <  1:311777/139‑1 (MQ=255)                                                                                                          cTTGCCGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCg                         <  2:704172/139‑1 (MQ=255)                                                                                                              ccGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              <  1:1117172/98‑1 (MQ=255)                                                                                                              ccGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGgcg                                                              >  2:1117172/1‑98 (MQ=255)                                                                                                               cGACCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAagag                    <  2:746595/139‑1 (MQ=255)                                                                                                                 aCCGTACCCACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCCCCACATTAAAACCGAACTCGAAGAGCt                  <  1:640904/139‑1 (MQ=255)                                                                                                                       cccACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCt                  >  2:553507/1‑133 (MQ=255)                                                                                                                       cccACAACATGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCt                  <  1:553507/133‑1 (MQ=255)                                                                                                                           caacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAg        <  2:412573/139‑1 (MQ=255)                                                                                                                           caacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAg        <  1:66277/139‑1 (MQ=255)                                                                                                                           caacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAg        <  1:622132/139‑1 (MQ=255)                                                                                                                            aacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            <  1:1245405/86‑1 (MQ=255)                                                                                                                            aacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCa                                                            >  2:1245405/1‑86 (MQ=255)                                                                                                                            aacaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTGTTGAgg       <  2:1199907/139‑1 (MQ=255)                                                                                                                             acaTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgc      >  1:153068/1‑139 (MQ=255)                                                                                                                              caTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgcg     <  1:275281/139‑1 (MQ=255)                                                                                                                               aTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgcgc    >  1:981646/1‑139 (MQ=255)                                                                                                                               aTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgcgc    <  1:351654/139‑1 (MQ=255)                                                                                                                               aTGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgcgc    <  2:483324/139‑1 (MQ=255)                                                                                                                                tGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCt   >  1:602482/1‑139 (MQ=255)                                                                                                                                tGCGCACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCt   <  2:882331/139‑1 (MQ=255)                                                                                                                                 gcgcACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTg  >  2:861823/1‑139 (MQ=255)                                                                                                                                  cgcACGTTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTg  <  1:315806/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    AAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTG  >  NZ_CP009273/3816063‑3816329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑