Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	339,921	T→C	M12T (ATG→ACG)	BW25113_RS01680 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	339,921	0	T	C	93.8%	260.6 / 5.3	80	M12T (ATG→ACG)	BW25113_RS01680	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (40/35);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CACAATACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTAT  >  NZ_CP009273/339786‑340051                                                                                                                                        |                                                                                                                                   cacaATACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGc                                                                                                                                  >  1:1025628/1‑138 (MQ=255)   caATACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCat                                                                                                                               <  2:1025628/139‑1 (MQ=255)   caATACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCat                                                                                                                               <  1:1113704/139‑1 (MQ=255)    aaTACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCata                                                                                                                              >  1:960030/1‑139 (MQ=255)    aaTACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCata                                                                                                                              >  1:657455/1‑139 (MQ=255)    aaTACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCata                                                                                                                              >  2:1157400/1‑139 (MQ=255)    aaTACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCata                                                                                                                              >  2:1252431/1‑139 (MQ=255)      tACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCAtata                                                                                                                            >  1:304353/1‑139 (MQ=255)      tACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCAtata                                                                                                                            >  1:1200304/1‑139 (MQ=255)      tACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCAtata                                                                                                                            <  2:657455/139‑1 (MQ=255)      tACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCAtata                                                                                                                            >  1:846268/1‑139 (MQ=255)         ttCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACg                                                                                                                          >  2:666195/1‑138 (MQ=255)         ttCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACg                                                                                                                          >  1:919651/1‑138 (MQ=255)         ttCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACg                                                                                                                          >  1:959899/1‑138 (MQ=255)           cacaCGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGcc                                                                                                                                 <  2:49172/129‑1 (MQ=255)           cacaCGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGcc                                                                                                                                 >  1:49172/1‑129 (MQ=255)           cacaCGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGcaa                                                                                                                       >  1:215398/1‑139 (MQ=255)             cacGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGcaaca                                                                                                                     <  1:427662/139‑1 (MQ=255)                 ttagttaTGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 <  1:825492/139‑1 (MQ=255)                 ttagttaTGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 <  2:223898/139‑1 (MQ=255)                 ttagttaTGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 <  2:391687/139‑1 (MQ=255)                  tagttaTGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCa                                                                                                                >  2:42374/1‑139 (MQ=255)                     ttaTGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 >  2:215146/1‑135 (MQ=255)                     ttaTGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 <  1:215146/135‑1 (MQ=255)                          aGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGcaac                                                                                                                      >  1:181953/1‑125 (MQ=255)                          aGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGcaac                                                                                                                      <  2:181953/125‑1 (MQ=255)                          aGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 <  2:197309/130‑1 (MQ=255)                          aGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 >  1:197309/1‑130 (MQ=255)                          aGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTaa                                                                                                         >  2:1007833/1‑138 (MQ=255)                            cGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGAt                                                                                                                                    >  1:396768/1‑109 (MQ=255)                            cGATTTCTGATAGTGCCTGGGTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGAt                                                                                                                                    <  2:396768/109‑1 (MQ=255)                              aTTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCAt                                                                                                               >  2:223951/1‑128 (MQ=255)                              aTTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCAt                                                                                                               <  1:223951/128‑1 (MQ=255)                                     aTAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTaaaaa                                                                                             <  2:1064030/139‑1 (MQ=255)                                      tAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAAc                                                                                            <  1:1157400/139‑1 (MQ=255)                                         tGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAAt                                                                                         >  2:803904/1‑139 (MQ=255)                                         tGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAAt                                                                                         <  2:392310/139‑1 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGcaaca                                                                                                                     >  1:745147/1‑109 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGcaaca                                                                                                                     <  2:745147/109‑1 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 <  2:928578/113‑1 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGc                                                                                                                 >  1:928578/1‑113 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAAt                                                                                         >  2:711292/1‑137 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAAt                                                                                         >  1:359639/1‑137 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCa                                                                                       <  2:372621/139‑1 (MQ=255)                                           ccTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCa                                                                                       <  1:563095/139‑1 (MQ=255)                                                    aTCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCag                                                                               <  2:919651/138‑1 (MQ=255)                                                    aTCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCa                                                                                >  1:1084767/1‑137 (MQ=255)                                                     tCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCagag                                                                             <  2:959899/139‑1 (MQ=255)                                                         agCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATa                                                                                                              >  2:259022/1‑102 (MQ=255)                                                         agCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATa                                                                                                              <  1:259022/102‑1 (MQ=255)                                                         agCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGata                                                                         >  1:590701/1‑139 (MQ=255)                                                         agCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGata                                                                         >  1:1074517/1‑139 (MQ=255)                                                          gCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGCTGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGatat                                                                        >  2:1012141/1‑139 (MQ=255)                                                              tattatCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCaa                                                                     >  1:1101043/1‑138 (MQ=255)                                                              tattatCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCaa                                                                     >  2:1124357/1‑138 (MQ=255)                                                              tattatCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCaa                                                                     >  2:344606/1‑138 (MQ=255)                                                               attatCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCaaa                                                                    <  1:1124357/138‑1 (MQ=255)                                                                   tCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCt                                                                >  2:643699/1‑138 (MQ=255)                                                                   tCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCt                                                                <  1:643699/138‑1 (MQ=255)                                                                            tttatttttattTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTc                                                      <  1:711292/139‑1 (MQ=255)                                                                                  tttatttaAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCtgtg                                                 <  1:344606/138‑1 (MQ=255)                                                                                      tttaAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGat                                            <  1:666195/139‑1 (MQ=255)                                                                                      tttaAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGa                                             >  1:1195762/1‑138 (MQ=255)                                                                                                      atgatATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGa                            <  2:215398/139‑1 (MQ=255)                                                                                                        gatATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCt                                                                >  2:388780/1‑101 (MQ=255)                                                                                                        gatATCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCt                                                                <  1:388780/101‑1 (MQ=255)                                                                                                            tCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTaaa                       <  1:1012141/138‑1 (MQ=255)                                                                                                            tCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTaaa                       <  1:1007833/138‑1 (MQ=255)                                                                                                            tCATTAAAAGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTaaa                       <  1:778061/138‑1 (MQ=255)                                                                                                               ttAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACaa                    >  1:1106021/1‑138 (MQ=255)                                                                                                                   aaGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTaaa                       >  1:1283846/1‑131 (MQ=255)                                                                                                                   aaGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTaaa                       <  2:1283846/131‑1 (MQ=255)                                                                                                                   aaGCTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAAtatta               >  1:369219/1‑139 (MQ=255)                                                                                                                      cTCCGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGa            <  2:590701/139‑1 (MQ=255)                                                                                                                        ccGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGt                                 <  1:937327/116‑1 (MQ=255)                                                                                                                        ccGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGt                                 >  2:937327/1‑116 (MQ=255)                                                                                                                        ccGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGAt           >  1:725678/1‑138 (MQ=255)                                                                                                                         cGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGAtt          <  1:1032811/138‑1 (MQ=255)                                                                                                                         cGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTa         >  1:698214/1‑139 (MQ=255)                                                                                                                         cGCACAATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTa         >  2:152354/1‑139 (MQ=255)                                                                                                                           cacaATAATTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATc       >  2:146777/1‑139 (MQ=255)                                                                                                                                taatTTGACGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTAt  <  2:1084767/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   CACAATACTTCACACGTTAGTTATGAGCGATTTCTGATAGTGCCTGGTTTAATCAGAGCTTTATTATCTGCGACGTTTATTTTTATTTAAGAGAGATCGCGATGATATCATTAAAAGCTCCGCACAATAATTTGATGCCATATACGCAACAAAGCATACTTAATACGGTTAAAAACAATCAGTTACCAGAGGATATCAAAAGCTCCCTGGTTTCCTGTGTCGATATATTCAAGGTTTTGATTAAACAATATTATGATTATCCTTAT  >  NZ_CP009273/339786‑340051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑