| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,931,799 | T→C | N71N (AAT→AAC) | rbsR → | ribose operon transcriptional repressor RbsR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,931,799 | 0 | T | C | 98.2% | 186.2 / ‑3.8 | 55 | N71N (AAT→AAC) | rbsR | ribose operon transcriptional repressor RbsR |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (23/31); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01 | |||||||||||
GCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAA > NZ_CP009273/3931663‑3931926 | gCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAAccc > 1:1247894/1‑139 (MQ=255) cGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCttt < 1:109529/138‑1 (MQ=255) cGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCttt < 2:1247894/138‑1 (MQ=255) cGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAAATCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCttt < 1:719364/138‑1 (MQ=255) aGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTAtt < 1:473257/139‑1 (MQ=255) aGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTAtt < 2:537173/139‑1 (MQ=255) aGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTAtt < 1:147386/139‑1 (MQ=255) tGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTAtt > 1:521378/1‑137 (MQ=255) aTTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTgg > 1:277098/1‑139 (MQ=255) aTTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTgg > 2:1260802/1‑139 (MQ=255) aCCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGc > 1:975746/1‑139 (MQ=255) ccGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCg < 1:662787/139‑1 (MQ=255) aaaGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCg < 1:956620/139‑1 (MQ=255) gAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCttt > 1:190335/1‑110 (MQ=255) gAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCttt < 2:190335/110‑1 (MQ=255) gAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGaa < 2:1265338/138‑1 (MQ=255) gATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAgctgc > 2:848669/1‑139 (MQ=255) aGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGaa < 1:1064394/139‑1 (MQ=255) aaCTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 1:993791/139‑1 (MQ=255) aaCTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 2:491068/139‑1 (MQ=255) aaCTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 1:1016557/139‑1 (MQ=255) aaCTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 2:983309/139‑1 (MQ=255) aaCTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 1:848669/139‑1 (MQ=255) aaCTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 1:17418/139‑1 (MQ=255) aaCTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 1:346384/139‑1 (MQ=255) gcgcCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGtc < 1:12386/139‑1 (MQ=255) gcgcCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGtc < 1:322503/139‑1 (MQ=255) cgcCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGtct > 1:297402/1‑139 (MQ=255) cATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGt > 1:598899/1‑139 (MQ=255) aTCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTGTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCCTCGAACGCGGTTATAGTCTCGTc < 2:910825/139‑1 (MQ=255) ggCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCaa > 1:854360/1‑138 (MQ=255) ggCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCaa > 1:244991/1‑138 (MQ=255) cgTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATAcc > 1:773329/1‑138 (MQ=255) tAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGaa < 2:600797/91‑1 (MQ=255) tAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGaa > 1:600797/1‑91 (MQ=255) tAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTta > 1:1036866/1‑114 (MQ=255) tAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTta < 2:1036866/114‑1 (MQ=255) aaaCACATACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGAt < 1:145295/139‑1 (MQ=255) aTACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg > 1:143933/1‑85 (MQ=255) aTACCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 2:143933/85‑1 (MQ=255) aCCATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGc > 1:1201656/1‑138 (MQ=255) cATTGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAAt < 1:785846/139‑1 (MQ=255) ttGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg < 2:491321/79‑1 (MQ=255) ttGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAAcg > 1:491321/1‑79 (MQ=255) ttGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCt > 1:1107737/1‑139 (MQ=255) ttGGCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCt > 2:888880/1‑139 (MQ=255) ggCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTg > 1:839106/1‑52 (MQ=255) ggCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTg < 2:839106/52‑1 (MQ=255) ggCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTg < 2:876805/52‑1 (MQ=255) ggCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTg > 1:876805/1‑52 (MQ=255) gCATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGa < 1:918396/139‑1 (MQ=255) cATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCg < 1:973323/129‑1 (MQ=255) cATGTTGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCg > 2:973323/1‑129 (MQ=255) ttGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGc > 2:1007643/1‑139 (MQ=255) ttGATCACTGCCAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGc > 2:951779/1‑139 (MQ=255) ccAGTACCAACCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCaaa > 2:299015/1‑138 (MQ=255) | GCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAA > NZ_CP009273/3931663‑3931926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |