Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,961,670 T→G V132G (GTG→GGG)  wecA → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,961,6700TG95.0% 211.4 / 2.2 60V132G (GTG→GGG) wecAUDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (31/26);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

ACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATT  >  NZ_CP009273/3961547‑3961803
                                                                                                                           |                                                                                                                                     
aCCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTgg                                                                                                                        <  1:181204/139‑1 (MQ=255)
      ttGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCg                                                                                                                                  >  1:1284121/1‑123 (MQ=255)
      ttGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCg                                                                                                                                  <  2:1284121/123‑1 (MQ=255)
          tatCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCt                                                                                                               >  2:1106553/1‑138 (MQ=255)
              aGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGc                                                                                                          >  2:371793/1‑139 (MQ=255)
                          cGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCt                                                                                              >  2:864697/1‑139 (MQ=255)
                          cGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCt                                                                                              >  2:657449/1‑139 (MQ=255)
                              ccaccaTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggc                                                                                          <  2:589344/139‑1 (MQ=255)
                              ccaccaTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggc                                                                                          <  1:314835/139‑1 (MQ=255)
                                 ccaTACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggcggc                                                                                       >  2:1259855/1‑139 (MQ=255)
                                      cAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTa                                                                                  <  1:1224866/139‑1 (MQ=255)
                                      cAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTa                                                                                  <  2:1090898/139‑1 (MQ=255)
                                         gCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGt                                                                                                                       <  2:934568/99‑1 (MQ=255)
                                         gCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGt                                                                                                                       >  1:934568/1‑99 (MQ=255)
                                         gCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATg                                                                               >  1:419711/1‑139 (MQ=255)
                                         gCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATg                                                                               >  1:881595/1‑139 (MQ=255)
                                           cGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggc                                                                                          >  2:806241/1‑126 (MQ=255)
                                           cGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggc                                                                                          <  1:806241/126‑1 (MQ=255)
                                           cGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCg                                                                             <  1:864697/139‑1 (MQ=255)
                                            gCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGa                                                                                                             >  2:668990/1‑106 (MQ=255)
                                            gCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGa                                                                                                             <  1:668990/106‑1 (MQ=255)
                                              tGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt                                                                           >  1:1208840/1‑138 (MQ=255)
                                                   gCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACa                                                                      <  2:1184634/138‑1 (MQ=255)
                                                     aTTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATgg                                                                   >  2:662011/1‑139 (MQ=255)
                                                            tgatgGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATg                                                             <  1:220686/138‑1 (MQ=255)
                                                              atgGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCa                                                          >  2:902073/1‑139 (MQ=255)
                                                                   gtTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTg                                                                                                              >  1:812703/1‑82 (MQ=255)
                                                                   gtTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTg                                                                                                              <  2:812703/82‑1 (MQ=255)
                                                                   gtTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTa                                                                                  >  1:929964/1‑110 (MQ=255)
                                                                   gtTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTa                                                                                  <  2:929964/110‑1 (MQ=255)
                                                                   gtTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGAt                                                     >  2:449681/1‑139 (MQ=255)
                                                                    ttCGGCAAGCTTTATCTCAGTAGCCTGGGTTATCTCTTTGGCTCCTGGGAGATGGGGTTCGGACCTTTTGGTTACTTCCTGCCGGTATGTGCCGTCTGGGCGCCGATTAATGCGTTCAACATGGTTGATGGCATTGAt                                                     <  2:1173006/138‑1 (MQ=255)
                                                                    gtCGGCATGCTTTATCTCAGTAGCCTGGGCTATATCTGTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTTTCGTCTGGGGGGCCAGTAATGCGTTCAACATGGTTGATGGCATTGAt                                                     <  2:1173033/137‑1 (MQ=255)
                                                                                tATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGc                                         <  2:243599/138‑1 (MQ=255)
                                                                                       gTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATgg                                                            <  2:915916/112‑1 (MQ=255)
                                                                                       gTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATgg                                                            >  1:915916/1‑112 (MQ=255)
                                                                                           ccTGGGTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGc                             >  1:1231960/1‑139 (MQ=255)
                                                                                              gggTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt                                                                           >  1:352185/1‑90 (MQ=255)
                                                                                              gggTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt                                                                           <  2:352185/90‑1 (MQ=255)
                                                                                               ggTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGt                           <  1:493394/137‑1 (MQ=255)
                                                                                               ggTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGt                           <  1:809608/137‑1 (MQ=255)
                                                                                                gTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggc                                                                                          >  1:772539/1‑73 (MQ=255)
                                                                                                gTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggc                                                                                          <  2:772539/73‑1 (MQ=255)
                                                                                                gTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGc                                              <  1:415909/117‑1 (MQ=255)
                                                                                                gTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGc                                              >  2:415909/1‑117 (MQ=255)
                                                                                                gTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtctc                        >  1:755631/1‑139 (MQ=255)
                                                                                                gTTATATCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtctc                        >  1:414839/1‑139 (MQ=255)
                                                                                                    tatCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt                                                                           <  1:446691/84‑1 (MQ=255)
                                                                                                    tatCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt                                                                           >  2:446691/1‑84 (MQ=255)
                                                                                                     atCTTTGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTg                   >  1:813452/1‑139 (MQ=255)
                                                                                                         ttGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgcag                >  2:348979/1‑138 (MQ=255)
                                                                                                          tGGCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTgca                 <  2:813452/136‑1 (MQ=255)
                                                                                                           ggCTCCTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCaa             >  2:981015/1‑139 (MQ=255)
                                                                                                                cTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGAc                                                                                                            <  2:31439/39‑1 (MQ=255)
                                                                                                                cTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGAc                                                                                                            >  1:31439/1‑39 (MQ=255)
                                                                                                                cTGGGAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCgg         >  1:573887/1‑138 (MQ=255)
                                                                                                                  gggAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtc                          >  1:292893/1‑119 (MQ=255)
                                                                                                                  gggAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtc                          <  2:292893/119‑1 (MQ=255)
                                                                                                                  gggAGATGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTAt      >  2:969317/1‑139 (MQ=255)
                                                                                                                      gaTGGGGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGAtt  <  1:662011/139‑1 (MQ=255)
                                                                                                                           |                                                                                                                                     
ACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATT  >  NZ_CP009273/3961547‑3961803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑