Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,994,362 T→C I19V (ATT→GTT)  yigE ← phosphodiester glycosidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,994,3620TC90.3% 271.7 / 17.1 93I19V (ATT→GTT) yigEphosphodiester glycosidase family protein
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base C (46/38);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01

TTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTG  >  NZ_CP009273/3994231‑3994494
                                                                                                                                   |                                                                                                                                    
ttGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGttt                                                                                                                                <  1:1210979/138‑1 (MQ=255)
     aGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGAtt                                                                                                                          >  1:237537/1‑139 (MQ=255)
      gTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTc                                                                                                                         <  2:804233/139‑1 (MQ=255)
         cATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAg                                                                                                                      >  1:329082/1‑139 (MQ=255)
          aTTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAgg                                                                                                                     <  2:329082/139‑1 (MQ=255)
            tttCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGt                                                                                                                    >  2:1186290/1‑138 (MQ=255)
            tttCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGt                                                                                                                    >  1:1261633/1‑138 (MQ=255)
               cACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGAt                                                                                                                 <  2:519806/138‑1 (MQ=255)
                        tGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCtt                                                                                                         <  1:638695/137‑1 (MQ=255)
                                 aTTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGt                                                                                              <  2:837362/139‑1 (MQ=255)
                                    aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAAt                                                                                                  <  1:561007/132‑1 (MQ=255)
                                    aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAAt                                                                                                  >  2:561007/1‑132 (MQ=255)
                                    aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGc                                                                                           >  1:1167614/1‑139 (MQ=255)
                                    aaCGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGCGATCATTCCTTTACCAATGAGTAGc                                                                                           >  2:841372/1‑139 (MQ=255)
                                         tataCGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATg                                                                                      <  1:877552/139‑1 (MQ=255)
                                         tataCGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATg                                                                                      <  2:2996/139‑1 (MQ=255)
                                                   tACGGTCAGCGTCGTATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  1:1157700/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTGCCAATGAGTAGCTGATGCGCCATta                                                                              >  2:1108074/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGGTGCGCCATta                                                                              >  2:235529/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgcg                                                                                    >  2:447672/1‑131 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgcg                                                                                    <  1:447672/131‑1 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgc                                                                                     >  1:748298/1‑130 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgc                                                                                     <  2:748298/130‑1 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATtaca                                                                            <  1:219650/139‑3 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATtaca                                                                            <  2:794860/139‑3 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  2:19679/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  1:180808/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  2:104852/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  2:219650/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  1:1231202/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  1:794860/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  1:851572/1‑137 (MQ=255)
                                                   tACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATta                                                                              >  1:1273148/1‑137 (MQ=255)
                                                       gTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTc                                                                        <  2:1167614/139‑1 (MQ=255)
                                                        tCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCa                                                                                                                        <  2:1183889/90‑1 (MQ=255)
                                                        tCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCa                                                                                                                        >  1:1183889/1‑90 (MQ=255)
                                                        tCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTcc                                                                       >  1:1206863/1‑139 (MQ=255)
                                                          aGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTg                                                                     >  1:876527/1‑139 (MQ=255)
                                                                  aTCTGAGAGTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTgg                                                              >  2:440586/1‑138 (MQ=255)
                                                                       agagTGCGCAATCATCAGCGTCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGATAAAAACCCGTTTGATATTCAAGGTGATCATTCCTTTACCAATGAGTACCTGATGCGCCATTACCGCTCCTGGATGTTGGa                                                            <  2:599080/135‑1 (MQ=255)
                                                                       agagTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGa                                                            <  2:876527/135‑1 (MQ=255)
                                                                       agagTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGAtttt                                                        >  1:132911/1‑139 (MQ=255)
                                                                       agagTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGAtt                                                          <  1:66382/137‑1 (MQ=255)
                                                                       agagTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGCGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGa                                                            <  1:841372/135‑1 (MQ=255)
                                                                         agTGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGAtttttt                                                      <  2:132911/139‑1 (MQ=255)
                                                                           tGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCa                                                                                                    <  2:1103701/91‑1 (MQ=255)
                                                                           tGCGCAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCa                                                                                                    >  1:1103701/1‑91 (MQ=255)
                                                                            gcgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTAt                                                   >  1:432393/1‑139 (MQ=255)
                                                                            gcgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTAt                                                   >  2:325374/1‑139 (MQ=255)
                                                                             cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCtt                                                                                                         <  2:1184737/84‑1 (MQ=255)
                                                                             cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCtt                                                                                                         >  1:1184737/1‑84 (MQ=255)
                                                                             cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATc                                                  >  1:1260187/1‑139 (MQ=255)
                                                                             cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATc                                                  >  2:1094046/1‑139 (MQ=255)
                                                                             cgcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATc                                                  >  1:692396/1‑139 (MQ=255)
                                                                             cgcAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCt                                                                                                          <  1:412971/83‑1 (MQ=255)
                                                                             cgcAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCt                                                                                                          >  2:412971/1‑83 (MQ=255)
                                                                              gcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATcc                                                 <  1:1241307/139‑1 (MQ=255)
                                                                              gcAATCATCAGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATcc                                                 <  2:598235/139‑1 (MQ=255)
                                                                                    atcaGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAg                                           <  2:1157700/139‑1 (MQ=255)
                                                                                       aGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATtac                                                                             >  1:158545/1‑101 (MQ=255)
                                                                                       aGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATtac                                                                             <  2:158545/102‑2 (MQ=255)
                                                                                       aGCGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGa                                        >  2:1195380/1‑139 (MQ=255)
                                                                                         cGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGAcc                                      <  2:432393/139‑1 (MQ=255)
                                                                                         cGGCGACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGAcc                                      >  1:378214/1‑139 (MQ=255)
                                                                                            cgaCAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATtac                                                                             <  2:467584/95‑2 (MQ=255)
                                                                                            cgaCAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATtac                                                                             >  1:467584/3‑96 (MQ=255)
                                                                                            cgaCAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCtt                                    >  1:166858/3‑138 (MQ=255)
                                                                                            cgaCAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCtt                                    >  1:1059931/3‑138 (MQ=255)
                                                                                              aCAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGa                                                                                        >  2:592391/1‑84 (MQ=255)
                                                                                              aCAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGa                                                                                        <  1:592391/84‑1 (MQ=255)
                                                                                                               tAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGcgcg                <  2:378214/139‑1 (MQ=255)
                                                                                                               tAAGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGcgcg                <  1:1001084/139‑1 (MQ=255)
                                                                                                                 aGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGcgcg                >  2:764258/1‑137 (MQ=255)
                                                                                                                 aGGTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGa               <  1:104852/138‑1 (MQ=255)
                                                                                                                  ggTGAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAg             <  1:35746/139‑1 (MQ=255)
                                                                                                                     gAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGAt                                                                                                                 <  2:902668/36‑1 (MQ=255)
                                                                                                                     gAGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGAt                                                                                                                 >  1:902668/1‑36 (MQ=255)
                                                                                                                      aGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAAt                                                                                                  >  1:1040075/1‑50 (MQ=255)
                                                                                                                      aGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAAt                                                                                                  <  2:1040075/50‑1 (MQ=255)
                                                                                                                      aGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTc          <  2:1206863/138‑1 (MQ=255)
                                                                                                                      aGGGCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTc          <  1:325374/138‑1 (MQ=255)
                                                                                                                        ggCGAGAAAAACCCGTTTGAGATTCGAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAAc       >  2:141545/1‑139 (MQ=255)
                                                                                                                        ggCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCtt                                                                                                         <  2:53225/41‑1 (MQ=255)
                                                                                                                        ggCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCtt                                                                                                         >  1:53225/1‑41 (MQ=255)
                                                                                                                        ggCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAg                                           >  2:290815/1‑103 (MQ=255)
                                                                                                                        ggCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAg                                           <  1:290815/103‑1 (MQ=255)
                                                                                                                         gCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgcg                                                                                    <  1:31274/61‑1 (MQ=255)
                                                                                                                         gCGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATgcg                                                                                    >  2:31274/1‑61 (MQ=255)
                                                                                                                          cGAGAAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTa     <  1:556592/139‑1 (MQ=255)
                                                                                                                            agaAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTc          >  2:1058478/1‑132 (MQ=255)
                                                                                                                            agaAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTc          <  1:1058478/132‑1 (MQ=255)
                                                                                                                            agaAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTAtt   >  2:1003862/1‑139 (MQ=255)
                                                                                                                             gaAAAACCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTACAGGTCCTGGATGTGGGATTTTTTTATCTTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTg  >  1:588935/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
TTGCCAGTACATTTTCACCCGCTCTGTTTGAGGATTAACGGTATACGCCTGTACGGTCAGCGTCGGATCTGAGAGTGCGCAATCATCAGCGGCAACAGCAAACAAGGGAAGTAAGGTGAGGGCGAGAAAAATCCGTTTGAGATTCAAGGTGATCATTCCTTTACCAATGAGTAGCTGATGCGCCATTATAGGTCCTGGATGTGGGATTTTTTTATCCTGTTAGCGACCTTGACGAGTACCAAAAAGCGCGAAGTTCAACTATTG  >  NZ_CP009273/3994231‑3994494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑