Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,995,690	C→T	A302V (GCG→GTG)	corA →	magnesium/cobalt transporter CorA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,995,690	0	C	T	100.0%	190.7 / NA	47	A302V (GCG→GTG)	corA	magnesium/cobalt transporter CorA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/25);  total (22/25)

CTCGGTGGTATCCGTGGTATTCCTGCCGCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAC  >  NZ_CP009273/3995562‑3995824                                                                                                                                 |                                                                                                                                       ctcGGTGGTATCCGTGGTATTCCTGCCGCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCt                                                                                                                                  <  1:1144862/135‑1 (MQ=255)         tATCCGTGGTATTCCTGCCGCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCt                                                                                                                      <  2:549312/139‑1 (MQ=255)                    ttCCTGCCGCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAg                                                                                                            <  1:236669/138‑1 (MQ=255)                      ccTGCCGCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCg                                                                                                          >  2:641949/1‑138 (MQ=255)                            gccgACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCAc                                                                                                                             >  2:4132/1‑113 (MQ=255)                            gccgACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCAc                                                                                                                             <  1:4132/113‑1 (MQ=255)                            gccgACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAAc                                                                                                   <  2:1130937/139‑1 (MQ=255)                                       ttGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTaaaa                                                                                        <  1:1285189/139‑1 (MQ=255)                                              cAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACgag                                                                                  <  1:811556/138‑1 (MQ=255)                                                    tGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTg                                                                           >  2:828630/1‑139 (MQ=255)                                                     ggCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTgg                                                                          >  2:123673/1‑139 (MQ=255)                                                      gCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACACTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGc                                                                         >  2:410004/1‑139 (MQ=255)                                                          gAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAg                                                                                                            >  1:403376/1‑100 (MQ=255)                                                          gAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAg                                                                                                            <  2:403376/100‑1 (MQ=255)                                                           aaCTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCAc                                                                                                                             >  1:662085/1‑82 (MQ=255)                                                           aaCTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCAc                                                                                                                             <  2:662085/82‑1 (MQ=255)                                                                       aTGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGcc                                                         <  1:878674/138‑1 (MQ=255)                                                                        tGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAc                                                       <  1:1097296/139‑1 (MQ=255)                                                                              aaCTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCAc                                                                                                                             <  1:416153/63‑1 (MQ=255)                                                                              aaCTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCAc                                                                                                                             >  2:416153/1‑63 (MQ=255)                                                                                   aaGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCg                                                                                                                           >  2:525519/1‑60 (MQ=255)                                                                                   aaGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCg                                                                                                                           <  1:525519/60‑1 (MQ=255)                                                                                    aGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCaaa                                           <  1:1014622/139‑1 (MQ=255)                                                                                    aGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCaaa                                           <  1:828630/139‑1 (MQ=255)                                                                                        gAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCt                                       >  2:1108538/1‑139 (MQ=255)                                                                                           cTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGt                                                                    >  2:1168229/1‑107 (MQ=255)                                                                                           cTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGt                                                                    <  1:1168229/107‑1 (MQ=255)                                                                                                   aCCCTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTAttt                             >  2:939915/1‑138 (MQ=255)                                                                                                    cccTGGCGCGATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTAttt                             <  1:646536/137‑1 (MQ=255)                                                                                                          cgcgATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCttt                     >  1:267514/1‑139 (MQ=255)                                                                                                          cgcgATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCttt                     >  1:368444/1‑139 (MQ=255)                                                                                                             gATTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTAc                  <  1:939915/139‑1 (MQ=255)                                                                                                              aTTATCTTTATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTAcc                 >  2:744721/1‑139 (MQ=255)                                                                                                                      tATGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCttt            <  1:936348/136‑1 (MQ=255)                                                                                                                       aTGATCCTCGTGGGCCTGCCACCGTCTCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCa        >  2:599813/1‑139 (MQ=255)                                                                                                                        tGATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTTTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCa        <  1:777057/138‑1 (MQ=255)                                                                                                                         gATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCg                                                                              <  1:44571/67‑1 (MQ=255)                                                                                                                         gATCCTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCg                                                                              >  2:44571/1‑67 (MQ=255)                                                                                                                            ccTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCgg                                                                             <  1:423332/65‑1 (MQ=255)                                                                                                                            ccTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCgg                                                                             >  2:423332/1‑65 (MQ=255)                                                                                                                            ccTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCaaa                                           >  1:1057005/1‑99 (MQ=255)                                                                                                                            ccTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCaaa                                           <  2:1057005/99‑1 (MQ=255)                                                                                                                            ccTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAg     <  2:415345/137‑1 (MQ=255)                                                                                                                            ccTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAg     >  1:415345/1‑137 (MQ=255)                                                                                                                             cTCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAc  >  1:893455/1‑139 (MQ=255)                                                                                                                              tCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGcc                                                         >  2:306182/1‑83 (MQ=255)                                                                                                                              tCGTGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGcc                                                         <  1:306182/83‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       CTCGGTGGTATCCGTGGTATTCCTGCCGCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAC  >  NZ_CP009273/3995562‑3995824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑