Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	345,822	A→T	T34S (ACC→TCC)	prpC →	2‑methylcitrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	345,822	0	A	T	87.0%	244.4 / 24.9	79	T34S (ACC→TCC)	prpC	2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (2/8);  new base T (38/29);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 4.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCT  >  NZ_CP009273/345689‑345956                                                                                                                                      |                                                                                                                                       cGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTg                                                                                                                                   >  1:1256287/1‑139 (MQ=255)  gACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTcc                                                                                                                                      <  2:1264160/135‑1 (MQ=255)     ccTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGt                                                                                                                                >  2:789534/1‑138 (MQ=255)        aCAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTATCTAGCGTTCCGTAGAGCAATACGACGCTCTGCGCCGTGGGTaa                                                                                                                              >  1:549358/1‑137 (MQ=255)               aTAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCa                                                                                                                      <  1:738769/138‑1 (MQ=255)                  aCAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATg                                                                                                                   <  2:289975/138‑1 (MQ=255)                   cAATGACGAGGACAACATGAGCGACACGACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 <  2:278155/139‑1 (MQ=255)                   cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 <  2:1120375/139‑1 (MQ=255)                   cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 <  2:1236187/139‑1 (MQ=255)                   cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 <  1:311439/139‑1 (MQ=255)                   cAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGAc                                                                                                                 <  1:269042/139‑1 (MQ=255)                      tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTg                                                                                                              >  1:559767/1‑139 (MQ=255)                      tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAcc                                                                                                                                      >  2:1248208/1‑115 (MQ=255)                      tGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCAcc                                                                                                                                      <  1:1248208/115‑1 (MQ=255)                        aCGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCa                                                                                                            >  2:679474/1‑139 (MQ=255)                        aCGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCa                                                                                                            >  1:1068764/1‑139 (MQ=255)                        aCGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCa                                                                                                            >  1:735404/1‑139 (MQ=255)                           aGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTa                                                                                                         <  1:599900/139‑1 (MQ=255)                              acaacaTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACcg                                                                                                      <  2:270826/139‑1 (MQ=255)                                  caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 >  2:316804/1‑124 (MQ=255)                                  caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 <  1:316804/124‑1 (MQ=255)                                  caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGc                                                                                                  >  1:371779/1‑139 (MQ=255)                                  caTGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGc                                                                                                  <  2:1256287/139‑1 (MQ=255)                                    tGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTa                                                                                                <  1:96902/139‑1 (MQ=255)                                      aGCGACACAACGATCCTTCCCATCTGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTTGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGTTTACATTTGGCACTTACCTGCATTACCGCTTCTACg                                                                                              <  1:534569/139‑1 (MQ=255)                                      aGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACg                                                                                              <  1:341939/139‑1 (MQ=255)                                      aGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACg                                                                                              <  2:956967/139‑1 (MQ=255)                                       gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATg                                                                                                                   <  1:810149/117‑1 (MQ=255)                                       gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATg                                                                                                                   >  2:810149/1‑117 (MQ=255)                                       gCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGTGGCTACGa                                                                                             <  2:604531/139‑1 (MQ=255)                                          acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGatat                                                                                          >  1:673553/1‑139 (MQ=255)                                          acacAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGatat                                                                                          >  2:192918/1‑139 (MQ=255)                                          acacAACGATCCTCCAAAACATGACCCTTGGCATTAAACCGACAAAATCTGTGGCACTCTCTGGCGTTCCGACCGGCAACACTACGCTCTCTTTCGCGGGTAAACGTGGCAATGAGCTGCATTACCGCGGCTACGatat                                                                                          >  2:810645/1‑139 (MQ=255)                                                  aTCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATc                                                                                  <  2:559767/139‑1 (MQ=255)                                                     cttcAAATCAGTACCCATGTCATTAAACCGAAAAAGTATGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAGAGTGGCAATGACCTGCATTACCGCGGCTACGCTATTCTGGATCTgg                                                                               >  2:676115/4‑139 (MQ=255)                                                     cTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTgg                                                                               >  1:181971/1‑139 (MQ=255)                                                        cAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGa                                                                            <  2:448051/139‑1 (MQ=255)                                                             cAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACAt                                                                       >  2:780019/1‑139 (MQ=255)                                                               gTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTg                                                                     >  2:194175/1‑139 (MQ=255)                                                               gTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTg                                                                     >  2:98365/1‑139 (MQ=255)                                                                tACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                                                    >  2:260090/1‑139 (MQ=255)                                                                tACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                                                    >  2:196148/1‑139 (MQ=255)                                                                tACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGc                                                                    >  2:1001797/1‑139 (MQ=255)                                                                       gTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 <  1:1018323/87‑1 (MQ=255)                                                                       gTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGAc                                                                                                                 >  2:1018323/1‑87 (MQ=255)                                                                       gTCATTAAACCGAAAAAATCTGCGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTg                                                             >  2:697354/1‑139 (MQ=255)                                                                          aTTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaa                                                           <  1:405844/138‑1 (MQ=255)                                                                           ttAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTgaaga                                                         <  2:371779/139‑1 (MQ=255)                                                                              aaCCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGt                                                      <  2:1237895/139‑1 (MQ=255)                                                                                ccGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTgg                                                    <  2:1068764/139‑1 (MQ=255)                                                                                   aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                 <  1:1241743/139‑1 (MQ=255)                                                                                   aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                 <  2:1075489/139‑1 (MQ=255)                                                                                   aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcgc                                                 <  1:84519/139‑1 (MQ=255)                                                                                   aaaaaaTCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGgcg                                                  >  1:770057/1‑138 (MQ=255)                                                                                                cACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCa                                    <  2:181971/139‑1 (MQ=255)                                                                                                   tttCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAtt                                                               <  1:1009751/109‑1 (MQ=255)                                                                                                   tttCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAAtt                                                               >  2:1009751/1‑109 (MQ=255)                                                                                                     tCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGc                                >  2:233392/1‑138 (MQ=255)                                                                                                     tCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGc                                >  1:114094/1‑138 (MQ=255)                                                                                                      cTGGCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCa                               <  1:368821/138‑1 (MQ=255)                                                                                                         gCGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCaaa                             <  1:192918/137‑1 (MQ=255)                                                                                                          cGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATg                                                                                                                   >  2:39766/1‑50 (MQ=255)                                                                                                          cGTTCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATg                                                                                                                   <  1:39766/50‑1 (MQ=255)                                                                                                            ttCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCa                               <  1:1026621/132‑1 (MQ=255)                                                                                                            ttCCGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCa                               >  2:1026621/1‑132 (MQ=255)                                                                                                              ccGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGa                      >  2:949989/1‑139 (MQ=255)                                                                                                              ccGGCGGGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGa                      >  2:801145/1‑139 (MQ=255)                                                                                                               cggcggGCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGAc                     >  1:814609/1‑139 (MQ=255)                                                                                                               ccgggggCAATTCGGCGCTTTGCGCCGTTGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGAc                     <  1:991330/135‑1 (MQ=255)                                                                                                                    ggCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                                                >  2:1079305/1‑107 (MQ=255)                                                                                                                    ggCAATACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCa                                                <  1:1079305/107‑1 (MQ=255)                                                                                                                         tACGGCGCTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGaa           >  2:182974/1‑139 (MQ=255)                                                                                                                              cgcTCTGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcgc      >  1:389329/1‑139 (MQ=255)                                                                                                                                ctctGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGTATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTcgccg    >  2:929647/1‑139 (MQ=255)                                                                                                                                   tGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACAtt                                                                      >  2:560311/1‑70 (MQ=255)                                                                                                                                   tGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACAtt                                                                      <  1:560311/70‑1 (MQ=255)                                                                                                                                   tGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCt  >  1:846340/1‑138 (MQ=255)                                                                                                                                   tGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCt  >  1:1048974/1‑138 (MQ=255)                                                                                                                                   tGCTCCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCt  >  2:1223571/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                       CGACCCTACAAATGATAACAATGACGAGGACAACATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCTGTGGCACTTTCTGGCGTTCCGGCGGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGCGGCTACGATATTCTTGATCTGGCGAAACATTGCGAATTTGAAGAAGTGGCGCATCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCT  >  NZ_CP009273/345689‑345956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑