| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,041,206 | G→A | G294D (GGC→GAC) | polA → | DNA polymerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,041,206 | 0 | G | A | 98.0% | 193.9 / ‑6.0 | 51 | G294D (GGC→GAC) | polA | DNA polymerase I |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (23/27); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGGCAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTTGATGAAGAAACAC > NZ_CP009273/4041071‑4041340 | tGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACa > 1:27089/1‑138 (MQ=255) gaTTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTa < 1:475472/139‑1 (MQ=255) gaTTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTa < 1:1210241/139‑1 (MQ=255) gaTTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTa < 2:1117514/139‑1 (MQ=255) cGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCaaa < 2:758841/139‑1 (MQ=255) aCGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAgg > 1:489457/1‑139 (MQ=255) gAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAg > 2:521224/1‑139 (MQ=255) aCCTGTGAACAACTGGAAGTGCAGCAACCGGCCGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCcgcg > 1:1130038/1‑138 (MQ=255) aCCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCcgcg > 1:767745/1‑138 (MQ=255) aCCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCcgc < 2:27089/137‑1 (MQ=255) cTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAg > 1:1204035/1‑139 (MQ=255) tgAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCca < 1:257290/139‑1 (MQ=255) tgAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCca > 2:137363/1‑139 (MQ=255) acaacTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAg < 2:780530/139‑1 (MQ=255) aacTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTa > 1:304551/1‑101 (MQ=255) aacTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTa < 2:304551/101‑1 (MQ=255) cTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGaaa < 2:1045748/139‑1 (MQ=255) cTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGaaa < 2:489457/139‑1 (MQ=255) gAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAAc > 1:359336/1‑137 (MQ=255) gAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCa < 1:624210/139‑1 (MQ=255) gTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAgtg < 2:767745/139‑1 (MQ=255) agcaACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGCAGCCACAGGAAACCAGTGTTg > 2:1153332/1‑138 (MQ=255) aaCCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGa < 2:1094192/139‑1 (MQ=255) cGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGa < 2:931421/139‑1 (MQ=255) cGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGa < 1:419746/139‑1 (MQ=255) gCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACg > 2:536394/1‑139 (MQ=255) ttCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGCAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCcac > 2:217973/1‑91 (MQ=255) ttCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCcac < 1:217973/91‑1 (MQ=255) ttCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGa > 1:258/1‑139 (MQ=255) aaaaaGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGAttt < 1:713955/139‑1 (MQ=255) aGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTa < 1:536394/139‑1 (MQ=255) tATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATg < 1:147213/139‑1 (MQ=255) aTGAGTTCAAACGCTGGACTGCTGATGTCTAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGa > 1:62051/1‑103 (MQ=255) aTGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGa < 2:62051/103‑1 (MQ=255) aTGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGa > 1:1107558/1‑129 (MQ=255) aTGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGa < 2:1107558/129‑1 (MQ=255) aTGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATg > 2:898737/1‑138 (MQ=255) aTGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATg < 1:898737/138‑1 (MQ=255) ttCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACt < 2:1204035/139‑1 (MQ=255) tCAAACGCTGGACTGCTGATGTCGAAGCGGACAAATGGTTCCGGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAATCAGTGTGGCAGACGAAGCCCCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTa < 2:65877/139‑1 (MQ=255) cAAACGCTGGACTGCTGATGTCGAAGCGGGCAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTa < 1:830629/138‑1 (MQ=255) cTGGACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGGGTTGCAGACGAAGCACCAGAAGTGACGGGAACGGTGATTTCTTATGACAACTACGTCAcc > 2:58839/1‑139 (MQ=255) ggACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGc > 1:690458/1‑106 (MQ=255) ggACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGc < 2:690458/106‑1 (MQ=255) ggACTGCTGATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCAt > 2:523259/1‑139 (MQ=255) ctgATGTCGAAGCGGACAAATGGTTACAGGCCGAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAg > 1:782405/1‑84 (MQ=255) ctgATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAg < 2:782405/84‑1 (MQ=255) ctgATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTtga > 1:837131/1‑139 (MQ=255) ctgATGTCGAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTtga > 1:6990/1‑139 (MQ=255) gAAGCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTTGATGAAGaaa > 1:795189/1‑139 (MQ=255) gCGGACAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTTGATGAAGAAacac < 2:1130038/139‑1 (MQ=255) ggCAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCca > 1:178320/1‑45 (MQ=255) ggCAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCca < 2:178320/45‑1 (MQ=255) | TGGCGACGATTAAAACCGACGTTGAACTGGAGCTGACCTGTGAACAACTGGAAGTGCAGCAACCGGCAGCGGAAGAGTTGTTGGGGCTGTTCAAAAAGTATGAGTTCAAACGCTGGACTGCTGATGTCGAAGCGGGCAAATGGTTACAGGCCAAAGGGGCAAAACCAGCCGCGAAGCCACAGGAAACCAGTGTTGCAGACGAAGCACCAGAAGTGACGGCAACGGTGATTTCTTATGACAACTACGTCACCATCCTTGATGAAGAAACAC > NZ_CP009273/4041071‑4041340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |