Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,077,063 T→A D707V (GAT→GTT)  fdnG ← formate dehydrogenase‑N subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,077,0630TA98.3% 236.9 / ‑3.9 60D707V (GAT→GTT) fdnGformate dehydrogenase‑N subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (25/34);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCAT  >  NZ_CP009273/4076927‑4077192
                                                                                                                                        |                                                                                                                                 
tGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACg                                                                                                                                 >  2:503327/1‑139 (MQ=255)
  ccATCTGGTTGCCTTCCGCCGTCCAGCTACTGGCGAAAATCCAGCAGCCCCAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATACCCGCACCGGTTGCCGGAACgg                                                                                                                                <  1:962115/138‑1 (MQ=255)
          ttGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCgg                                                                                                                       >  2:1204978/1‑139 (MQ=255)
          ttGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCgg                                                                                                                       >  2:431676/1‑139 (MQ=255)
          ttGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCgg                                                                                                                       >  1:140010/1‑139 (MQ=255)
          ttGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCgg                                                                                                                       >  2:479219/1‑139 (MQ=255)
             ccTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGcc                                                                                                                     <  1:1053553/138‑1 (MQ=255)
              cTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAg                                                                                                                   <  2:752255/139‑1 (MQ=255)
               ttCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAgc                                                                                                                  >  2:258933/1‑139 (MQ=255)
                     cGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTa                                                                                                            <  1:503327/139‑1 (MQ=255)
                      gTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTAc                                                                                                           <  2:140010/139‑1 (MQ=255)
                          aGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGtt                                                                                                       <  1:297231/139‑1 (MQ=255)
                            cTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGc                                                                                                     >  1:1201150/1‑139 (MQ=255)
                              aCCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGctc                                                                                                   >  1:404613/1‑139 (MQ=255)
                                         tCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCAc                                                                                        <  1:431676/139‑1 (MQ=255)
                                         tCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCAc                                                                                        <  1:1066437/139‑1 (MQ=255)
                                         tCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCAc                                                                                        <  2:1201150/139‑1 (MQ=255)
                                                      ggaggTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGAt                                                                           <  1:1030598/139‑1 (MQ=255)
                                                         ggTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGc                                                                        <  1:848618/139‑1 (MQ=255)
                                                         ggTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGc                                                                        <  1:523654/139‑1 (MQ=255)
                                                           ttGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCt                                                                       >  1:1187288/1‑138 (MQ=255)
                                                           ttGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCt                                                                       >  2:315099/1‑138 (MQ=255)
                                                            tGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCa                                                                                                                    >  1:306611/1‑92 (MQ=255)
                                                            tGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCa                                                                                                                    <  2:306611/92‑1 (MQ=255)
                                                                ccGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCg                                                                 <  1:1010257/139‑1 (MQ=255)
                                                                   tCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              <  1:1027774/139‑1 (MQ=255)
                                                                   tCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              >  1:1040078/1‑139 (MQ=255)
                                                                   tCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              <  1:791855/139‑1 (MQ=255)
                                                                   tCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              <  2:271117/139‑1 (MQ=255)
                                                                    cATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATg                                                                   >  1:994949/1‑133 (MQ=255)
                                                                    cATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATg                                                                   <  2:994949/133‑1 (MQ=255)
                                                                     aTCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              <  1:994283/137‑1 (MQ=255)
                                                                     aTCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              >  2:994283/1‑137 (MQ=255)
                                                                        gcgcAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCg                                                         <  1:1204978/139‑1 (MQ=255)
                                                                         cgcAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              >  1:384946/1‑133 (MQ=255)
                                                                         cgcAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGaa                                                              <  2:384946/133‑1 (MQ=255)
                                                                         cgcAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGt                                                        <  2:404613/139‑1 (MQ=255)
                                                                                  gCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCcatg                                               <  1:474972/139‑1 (MQ=255)
                                                                                    gaacgaacTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCcatgtc                                             <  2:1153791/139‑1 (MQ=255)
                                                                                    gaacgaacTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCcatgtc                                             <  1:485331/139‑1 (MQ=255)
                                                                                      acgaacTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCcatgtcat                                           >  1:538616/1‑139 (MQ=255)
                                                                                                   ttgGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTgg                              >  1:1011488/1‑139 (MQ=255)
                                                                                                   ttgGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTgg                              >  1:810742/1‑139 (MQ=255)
                                                                                                          ttCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGt                       >  2:1154059/1‑139 (MQ=255)
                                                                                                           tCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGtt                      >  2:770358/1‑139 (MQ=255)
                                                                                                           tCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGt                       <  1:479219/138‑1 (MQ=255)
                                                                                                           tCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGt                       <  2:1040078/138‑1 (MQ=255)
                                                                                                           tCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGt                       <  2:978954/138‑1 (MQ=255)
                                                                                                           tCTTGACGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGt                       <  2:177896/138‑1 (MQ=255)
                                                                                                                aCGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTcgcg                  <  2:854246/138‑1 (MQ=255)
                                                                                                                aCGATAACCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTcgcg                  <  2:26435/138‑1 (MQ=255)
                                                                                                                     aaCCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCg                                                         <  2:660915/94‑1 (MQ=255)
                                                                                                                     aaCCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCg                                                         >  1:660915/1‑94 (MQ=255)
                                                                                                                     aaCCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCAccc            >  1:782262/1‑139 (MQ=255)
                                                                                                                     aaCCGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCAccc            >  2:970335/1‑139 (MQ=255)
                                                                                                                       ccGCACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTg          >  2:922398/1‑139 (MQ=255)
                                                                                                                          cACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTc       >  1:46661/1‑139 (MQ=255)
                                                                                                                          cACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCAcc                                 <  2:544734/113‑1 (MQ=255)
                                                                                                                          cACCGGTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCAcc                                 >  1:544734/1‑113 (MQ=255)
                                                                                                                               gTTGCCGGAACGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCAt  <  1:1199954/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
TGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCGGAATGGCGTAGTTCCATGTCATGTTCAGCACCTGGTCCGGGTTCGCGCCACCCTGTTCGGCAT  >  NZ_CP009273/4076927‑4077192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑