Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,078,866 C→T S106N (AGC→AAC)  fdnG ← formate dehydrogenase‑N subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,078,8660CT95.4% 309.3 / 2.4 87S106N (AGC→AAC) fdnGformate dehydrogenase‑N subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (37/46);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

TTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTAC  >  NZ_CP009273/4078730‑4079000
                                                                                                                                        |                                                                                                                                      
ttCGGCGTTTTGCGCAATGCAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGttt                                                                                                                                      >  2:127210/1‑139 (MQ=255)
   ggCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCg                                                                                                                                   <  1:337402/139‑1 (MQ=255)
     cGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGa                                                                                                                                 <  1:627937/139‑1 (MQ=255)
               aaTGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGaaa                                                                                                                       <  1:1201951/139‑1 (MQ=255)
                     gTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATccac                                                                                                                  <  2:885114/138‑1 (MQ=255)
                        aGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAgg                                                                                                              >  1:921602/1‑139 (MQ=255)
                         gCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGc                                                                                                             <  1:710080/139‑1 (MQ=255)
                           aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATccac                                                                                                                  >  2:839964/1‑132 (MQ=255)
                           aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATccac                                                                                                                  <  1:839964/132‑1 (MQ=255)
                           aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGc                                                                                                             >  1:688820/1‑137 (MQ=255)
                           aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGc                                                                                                             >  2:1065480/1‑137 (MQ=255)
                           aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGc                                                                                                             >  1:396414/1‑137 (MQ=255)
                             cgcgGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAgc                                                                                                         >  1:708781/1‑139 (MQ=255)
                              gcgGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGGCATTTATCAGAGCCTGGCGCACGATATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAgc                                                                                                         <  2:116159/138‑1 (MQ=255)
                              gcgGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAgc                                                                                                         <  1:1067030/138‑1 (MQ=255)
                                    ttcttTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGaaa                                                                                                                       <  1:513302/118‑1 (MQ=255)
                                    ttcttTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGaaa                                                                                                                       >  2:513302/1‑118 (MQ=255)
                                    ttcttTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTc                                                                                                  >  1:1210039/1‑139 (MQ=255)
                                         tcatcaGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCgg                                                                                                <  1:430094/136‑1 (MQ=255)
                                          catcaGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACa                                                                                             <  1:299643/138‑1 (MQ=255)
                                            tcaGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAg                                                                                          >  2:786730/1‑139 (MQ=255)
                                                tttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGaa                                                                                                                        <  1:888490/105‑1 (MQ=255)
                                                tttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGaa                                                                                                                        >  2:888490/1‑105 (MQ=255)
                                                tttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                                                                      >  1:90482/1‑139 (MQ=255)
                                                tttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                                                                      <  1:779377/139‑1 (MQ=255)
                                                tttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                                                                      >  1:137147/1‑139 (MQ=255)
                                                  tGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTACTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCAc                                                                                     <  2:270803/138‑1 (MQ=255)
                                                         gcgatCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGtt                                                                              >  1:572894/1‑138 (MQ=255)
                                                               aaaCGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCgg                                                                        <  2:688820/138‑1 (MQ=255)
                                                               aaaCGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCgg                                                                        <  2:137147/138‑1 (MQ=255)
                                                                       ctTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCg                                                                >  2:806774/1‑138 (MQ=255)
                                                                       ctTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCg                                                                >  2:943906/1‑138 (MQ=255)
                                                                          cccagttGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              <  1:545230/131‑1 (MQ=255)
                                                                          cccAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              <  1:31879/137‑1 (MQ=255)
                                                                          cccAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              <  1:943906/137‑1 (MQ=255)
                                                                             aaCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGcc                                                         <  1:110024/139‑1 (MQ=255)
                                                                             aaCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGcc                                                         <  1:640562/139‑1 (MQ=255)
                                                                              aCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACcgc                                                                                  <  1:103478/113‑1 (MQ=255)
                                                                              aCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACcgc                                                                                  >  2:823473/1‑113 (MQ=255)
                                                                              aCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACcgc                                                                                  >  2:103478/1‑113 (MQ=255)
                                                                              aCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACcgc                                                                                  <  1:823473/113‑1 (MQ=255)
                                                                               cTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCtt                                                       <  2:256700/139‑1 (MQ=255)
                                                                                  aTTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCt                                                        >  2:855016/1‑135 (MQ=255)
                                                                                    ttGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  >  2:777064/1‑139 (MQ=255)
                                                                                    ttGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  >  2:563381/1‑139 (MQ=255)
                                                                                     tgctgcCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  <  1:1124972/138‑1 (MQ=255)
                                                                                     tgctgcCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  <  1:777064/138‑1 (MQ=255)
                                                                                       ctgcCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATg                                                <  2:283764/138‑1 (MQ=255)
                                                                                          ccATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGa                                              <  1:1065480/137‑1 (MQ=255)
                                                                                                    gAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAgc                                                                                                         >  2:497246/1‑68 (MQ=255)
                                                                                                    gAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAgc                                                                                                         <  1:497246/68‑1 (MQ=255)
                                                                                                       ccTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTgg                               >  1:479206/1‑139 (MQ=255)
                                                                                                           gcgcACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGcc                                                         >  1:760472/1‑109 (MQ=255)
                                                                                                           gcgcACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGcc                                                         <  2:760472/109‑1 (MQ=255)
                                                                                                            cgcACGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGttt                          >  2:458533/1‑139 (MQ=255)
                                                                                                                cGGTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTg                      <  1:1277949/139‑1 (MQ=255)
                                                                                                                 ggTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGc                     >  1:174098/1‑139 (MQ=255)
                                                                                                                  gTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCt                    <  2:741475/139‑1 (MQ=255)
                                                                                                                  gTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCt                    <  2:921602/139‑1 (MQ=255)
                                                                                                                  gTATTCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCt                    <  2:174098/139‑1 (MQ=255)
                                                                                                                     ttCCGGAAACTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTcc                  <  2:572894/138‑1 (MQ=255)
                                                                                                                           aaaCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  <  1:506882/100‑1 (MQ=255)
                                                                                                                           aaaCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  >  2:506882/1‑100 (MQ=255)
                                                                                                                           aaaCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCAcc            >  2:1243304/1‑138 (MQ=255)
                                                                                                                             aCTTCAGACGTTTTTCGGAGTGGATGAAATCCACTAGGCCAgc                                                                                                         <  2:454928/43‑1 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATTAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              <  2:1058950/86‑1 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATTAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa                                                              >  1:1058950/1‑86 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAgc                                                                                                         >  1:454928/1‑43 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACcgcg                                                                                 >  2:857918/1‑67 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACcgcg                                                                                 <  1:857918/67‑1 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  >  1:1044842/1‑98 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGata                                                  <  2:1044842/98‑1 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  2:292537/1‑139 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  1:661843/1‑139 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  2:204027/1‑139 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  2:1226803/1‑139 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  1:752342/1‑139 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  1:744784/1‑139 (MQ=255)
                                                                                                                             aCTTCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAg         >  2:1051309/1‑139 (MQ=255)
                                                                                                                               ttCAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       >  1:881793/1‑139 (MQ=255)
                                                                                                                                 cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  1:741310/137‑1 (MQ=255)
                                                                                                                                 cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  2:487569/137‑1 (MQ=255)
                                                                                                                                 cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  2:1081456/137‑1 (MQ=255)
                                                                                                                                 cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  1:42500/137‑1 (MQ=255)
                                                                                                                                 cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  1:681704/137‑1 (MQ=255)
                                                                                                                                 cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  1:292537/137‑1 (MQ=255)
                                                                                                                                 cAGACGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGc       <  2:396414/137‑1 (MQ=255)
                                                                                                                                    aCGGTTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTAGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTAc  <  2:752342/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTAC  >  NZ_CP009273/4078730‑4079000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑