Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,359,148 G→A A361V (GCA→GTA)  yjeH ← L‑methionine/branched‑chain amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,359,1480GA98.1% 194.9 / ‑4.2 53A361V (GCA→GTA) yjeHL‑methionine/branched‑chain amino acid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (19/33);  total (19/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

AGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCA  >  NZ_CP009273/4359013‑4359279
                                                                                                                                       |                                                                                                                                   
agcaCCAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCa                                                                                                                                  <  1:412647/139‑1 (MQ=255)
agcaACAGCCATACCCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCa                                                                                                                                  <  1:1012910/139‑1 (MQ=255)
  aaccgcccATACCCCCGCCAGCATGATAAGCGCATAGAGACTTTCCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAgc                                                                                                                                <  2:912811/133‑1 (MQ=255)
        ccATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAgcatgcat                                                                                                                          <  1:821472/139‑1 (MQ=255)
         caaacccccGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGCCCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATa                                                                                                                         <  2:1137571/134‑1 (MQ=255)
         cATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATa                                                                                                                         <  2:457334/139‑1 (MQ=255)
                 cGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATa                                                                                                                  >  1:1027496/1‑138 (MQ=255)
                  gCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCa                                                                                                                                  <  1:610474/121‑1 (MQ=255)
                  gCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCa                                                                                                                                  >  2:610474/1‑121 (MQ=255)
                  gCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATaaa                                                                                                                >  1:1029940/1‑139 (MQ=255)
                   ccAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAAt                                                                                                               >  1:732927/1‑139 (MQ=255)
                       cATGATCAGCGCATAGAGCCTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCata                                                                                                           <  2:386232/139‑1 (MQ=255)
                       cATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAGCATGCATAACAGATAAATCata                                                                                                           <  1:244634/139‑1 (MQ=255)
                       cATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCata                                                                                                           <  1:784440/139‑1 (MQ=255)
                            tCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCataata                                                                                                        >  2:701178/1‑137 (MQ=255)
                            tCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATaaa                                                                                                      <  1:840174/139‑1 (MQ=255)
                              aGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGa                                                                                                    <  1:749848/139‑1 (MQ=255)
                                cgcATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAgcatg                                                                                                                             >  2:769343/1‑112 (MQ=255)
                                cgcATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAgcatg                                                                                                                             <  1:769343/112‑1 (MQ=255)
                                cgcATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATg                                                                                                  >  1:1223555/1‑139 (MQ=255)
                                 gcATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGc                                                                                                 >  1:367061/1‑139 (MQ=255)
                                     agagACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCAtt                                                                                             >  1:230964/1‑139 (MQ=255)
                                       agaCTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTgg                                                                                           >  1:205367/1‑139 (MQ=255)
                                         aCTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCa                                                                                         <  2:205367/139‑1 (MQ=255)
                                           ttttCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCat                                                                                        >  1:927723/1‑138 (MQ=255)
                                             ttCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCataaa                                                                                     >  2:834327/1‑139 (MQ=255)
                                             ttCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCataaa                                                                                     >  1:1188450/1‑139 (MQ=255)
                                                   ccGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAaataat                                                                                 >  1:118044/1‑137 (MQ=255)
                                                     gACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAaga                                                                             <  2:757551/139‑1 (MQ=255)
                                                        cATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCg                                                                          <  1:975487/139‑1 (MQ=255)
                                                         aTTGCCAGTAACAGAACGCAAAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGt                                                                         <  2:367061/139‑1 (MQ=255)
                                                          ttGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTc                                                                        <  2:344586/139‑1 (MQ=255)
                                                                gTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGAtt                                                                  <  2:1029940/139‑1 (MQ=255)
                                                                gTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGAtt                                                                  <  1:52900/139‑1 (MQ=255)
                                                                 tAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGAtt                                                                  >  1:1032479/1‑138 (MQ=255)
                                                                 tAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTg                                                                 <  2:1188450/139‑1 (MQ=255)
                                                                   aCAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGa                                                                <  2:1223555/138‑1 (MQ=255)
                                                                   aCAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGAt                                                               <  2:1035651/139‑1 (MQ=255)
                                                                           cATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAg                                                       <  1:161577/139‑1 (MQ=255)
                                                                            aTAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAg                                                       >  2:675123/1‑138 (MQ=255)
                                                                               aCAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATg                                                   <  1:675123/139‑1 (MQ=255)
                                                                                  gCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAAt                                                <  2:118044/139‑1 (MQ=255)
                                                                                   cccGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCa                                                                                                                                  >  2:471048/1‑56 (MQ=255)
                                                                                   cccGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCa                                                                                                                                  <  1:471048/56‑1 (MQ=255)
                                                                                   cccGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCa                                                                                         >  2:501211/1‑97 (MQ=255)
                                                                                   cccGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCa                                                                                         <  1:501211/97‑1 (MQ=255)
                                                                                     cGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCAc                                             >  1:74562/1‑139 (MQ=255)
                                                                                         aaCCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATTAAGATGCCAATGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCaaa                                         <  1:1121716/139‑1 (MQ=255)
                                                                                         aaCCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCaaa                                         <  2:1032479/139‑1 (MQ=255)
                                                                                                           aTAACGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACAcagcag                       <  1:704002/139‑1 (MQ=255)
                                                                                                               cGTCCTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGa                   <  1:31558/139‑1 (MQ=255)
                                                                                                                   cTTGCAATAATTTACAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCAc               <  2:293882/139‑1 (MQ=255)
                                                                                                                                 cAGCCTACCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCa  <  1:419834/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
AGCAACAGCCATAACCCCGCCAGCATGATCAGCGCATAGAGACTTTTCCAGCCGACCATTGCCAGTAACAGAACGCATAACAGCCCGCCAACCACCGCCAGTAGTCGATAACGTCCTTGCAATAATTTACAGCCTGCCAGCATGCATAACAGATAAATCATAATAAAGATGCCATTGGCATAAATAATAAGAGCGTCCAGATTGATCTCTAAAGCATGAATCACCAAAGTGCTCACCACACAGCAGCCGAGCACCGCATTGAGGGCA  >  NZ_CP009273/4359013‑4359279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑