| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,443,731 | C→T | A111V (GCC→GTC) | yjfF → | sugar ABC transporter permease YjfF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,443,731 | 0 | C | T | 97.7% | 152.5 / ‑3.6 | 44 | A111V (GCC→GTC) | yjfF | sugar ABC transporter permease YjfF |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (23/20); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01 | |||||||||||
CGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAA > NZ_CP009273/4443599‑4443865 | cggtGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGATGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCAGTTATGGGGCTTCTGATAGACGTCCTGac > 2:864563/1‑138 (MQ=255) gttGATCGCCTTTCCTGGCGTGTTTCTGGAAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGTTGGGCTGTGCCTTCGTCGCATTTATGGGGCTTCTGATTGACGTCCTGaa < 2:910980/136‑1 (MQ=255) aTCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATcc < 2:631652/139‑1 (MQ=255) aTCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATcc < 2:1095616/139‑1 (MQ=255) aTCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATc < 1:424128/138‑1 (MQ=255) gCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCAt > 1:972054/1‑139 (MQ=255) gtgtTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTa > 2:1250326/1‑139 (MQ=255) tttCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGc > 1:922366/1‑139 (MQ=255) tttCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGc > 1:539190/1‑139 (MQ=255) gCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCg < 2:655516/138‑1 (MQ=255) gCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCg < 1:875121/138‑1 (MQ=255) tCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTAc < 2:1136580/87‑1 (MQ=255) tCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTAc > 1:1136580/1‑87 (MQ=255) cTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGa < 1:46278/139‑1 (MQ=255) cTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGa < 1:341556/139‑1 (MQ=255) gTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGAt < 1:99022/137‑1 (MQ=255) gTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGAt < 2:1174487/137‑1 (MQ=255) tGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTACGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTcc > 1:908174/1‑139 (MQ=255) ggTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGAt > 1:1177157/1‑138 (MQ=255) gTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATa > 1:664616/1‑138 (MQ=255) tGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATaaa < 2:924795/139‑1 (MQ=255) tGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATaaa > 1:273822/1‑139 (MQ=255) tGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCAtt < 2:14881/61‑1 (MQ=255) tGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCAtt > 1:14881/1‑61 (MQ=255) ggCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCAt < 2:1070998/138‑1 (MQ=255) gtgCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGa > 1:539306/1‑112 (MQ=255) gtgCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGa < 2:539306/112‑1 (MQ=255) gtgCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCAt > 1:840810/1‑139 (MQ=255) tgCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCAtt > 1:655046/1‑139 (MQ=255) tgCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCAtt > 1:1187809/1‑139 (MQ=255) tgCCTTCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCAtt > 2:268074/1‑139 (MQ=255) tCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGc < 1:66457/46‑1 (MQ=255) tCGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGc > 2:66457/1‑46 (MQ=255) cGGCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGa > 2:44648/1‑138 (MQ=255) ggCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGaca > 1:793251/1‑139 (MQ=255) ggCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGaca < 2:857541/139‑1 (MQ=255) ggCGCATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGaca < 1:444515/139‑1 (MQ=255) cgcATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACg < 2:426640/139‑1 (MQ=255) cgcATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACg < 1:650923/139‑1 (MQ=255) cgcATTTATGGGGCTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGACGATTCCGATAAACCATCCCATTTATGACACg < 1:1105638/139‑1 (MQ=255) cTTCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTg > 1:775860/1‑139 (MQ=255) tCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCg > 1:1204580/1‑139 (MQ=255) tCTGATCGACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCg > 2:730331/1‑139 (MQ=255) gACGTCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGaaaa > 1:1277828/1‑139 (MQ=255) | CGGTGATCGCCTTTACTGGCGTGTTTCTGGCAAAAGTGATTGGCGATTTCGGCCTCTCGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAA > NZ_CP009273/4443599‑4443865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |