Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,469,838	C→T	L98F (CTT→TTT)	yjgN →	YjgN family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,469,838	0	C	T	94.3%	242.3 / 3.1	70	L98F (CTT→TTT)	yjgN	YjgN family protein
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (38/28);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

ATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTC  >  NZ_CP009273/4469704‑4469972                                                                                                                                        |                                                                                                                                       aTCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTttgt                                                                                                                                    >  2:334292/1‑139 (MQ=255)  tCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTttgt                                                                                                                                    <  1:58561/138‑1 (MQ=255)       aTGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTgttggttg                                                                                                                              >  1:958393/1‑139 (MQ=255)       aTGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTgttggtt                                                                                                                               <  1:453906/138‑1 (MQ=255)       aTGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTgttggtt                                                                                                                               <  2:172330/138‑1 (MQ=255)            aaTATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGttt                                                                                                                         >  1:62207/1‑139 (MQ=255)              tatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTgttggt                                                                                                                                <  1:353566/130‑1 (MQ=255)              tatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTgttggt                                                                                                                                >  2:353566/1‑130 (MQ=255)              tatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGtttt                                                                                                                        >  2:1251235/1‑138 (MQ=255)              tatGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGtttt                                                                                                                        >  1:321870/1‑138 (MQ=255)                  gAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACtt                                                                                                                   <  1:108893/139‑1 (MQ=255)                     gTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGt                                                                                                                >  2:1176678/1‑139 (MQ=255)                       tAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTgttggt                                                                                                                                >  1:744692/1‑121 (MQ=255)                       tAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTgttggt                                                                                                                                <  2:744692/121‑1 (MQ=255)                        aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTa                                                                                                              >  2:1275818/1‑139 (MQ=255)                        aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTAc                                                                                                             >  2:668027/1‑139 (MQ=255)                        aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTAc                                                                                                             >  1:508350/1‑139 (MQ=255)                        aaCGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTAc                                                                                                             >  2:768957/1‑139 (MQ=255)                         aCGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACt                                                                                                            >  1:433853/1‑139 (MQ=255)                               aaCGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGt                                                                                                      >  1:208567/1‑139 (MQ=255)                               aaCGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGt                                                                                                      >  2:6304/1‑139 (MQ=255)                                aCGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGtt                                                                                                     >  1:283338/1‑139 (MQ=255)                                 cGATTTTCTTATGGAATTACCGGTGGGAATGGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGtt                                                                                                     <  1:1275818/139‑1 (MQ=255)                                 cGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGttt                                                                                                    <  2:208567/139‑1 (MQ=255)                                      ttCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCt                                                                                                <  1:668027/138‑1 (MQ=255)                                                 ttACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATgg                                                                                     >  2:361950/1‑138 (MQ=255)                                                 ttACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATgg                                                                                     >  1:791893/1‑138 (MQ=255)                                                   aCCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGgcag                                                                                  >  1:735592/1‑139 (MQ=255)                                                        tGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCaa                                                                               >  2:550093/1‑137 (MQ=255)                                                                 tttttGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGc                                                                      <  2:144480/138‑1 (MQ=255)                                                                   tttGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGt                                                                    >  1:466231/1‑138 (MQ=255)                                                                       ttAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGtcatc                                                                <  2:65658/138‑1 (MQ=255)                                                                           ttGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGcc                                                           >  1:283746/1‑139 (MQ=255)                                                                                   tttttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAc                                                   >  1:953234/1‑139 (MQ=255)                                                                                     tttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAcc                                                  <  1:435664/138‑1 (MQ=255)                                                                                     tttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAcc                                                  <  2:702834/138‑1 (MQ=255)                                                                                     tttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAcc                                                  <  2:697878/138‑1 (MQ=255)                                                                                             tttCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCaa                                          <  2:508350/138‑1 (MQ=255)                                                                                               tCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACg                                        <  1:361950/138‑1 (MQ=255)                                                                                                                   aCAGTGTCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAAt                    <  2:735592/138‑1 (MQ=255)                                                                                                                      gtgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                <  2:433853/139‑1 (MQ=255)                                                                                                                      gtgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                <  1:550093/139‑1 (MQ=255)                                                                                                                      gtgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                <  2:283338/139‑1 (MQ=255)                                                                                                                      gtgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                <  1:334292/139‑1 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGTCTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  1:1119444/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                                                                               >  2:390146/1‑59 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                                                                               <  1:390146/59‑1 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCaa                                                                               >  1:1050414/1‑75 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCaa                                                                               <  2:1050414/75‑1 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAcc                                                  <  2:1110948/104‑1 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTaa                     <  2:29176/133‑1 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTaa                     >  1:29176/1‑133 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  1:224793/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  2:215274/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  1:1226594/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  1:611509/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  2:1099808/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  1:736462/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGTTTGTTGGTTGTGTTGTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGAcc                                                  >  1:1110948/1‑104 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  1:420076/1‑139 (MQ=255)                                                                                                                       tgtCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctc               >  2:417196/1‑139 (MQ=255)                                                                                                                         tCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTg                  <  2:561544/134‑1 (MQ=255)                                                                                                                         tCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTg                  >  1:561544/1‑134 (MQ=255)                                                                                                                         tCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                >  2:596772/1‑136 (MQ=255)                                                                                                                         tCAGCAGATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                <  1:596772/136‑1 (MQ=255)                                                                                                                              agATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGaaa        <  2:669515/139‑1 (MQ=255)                                                                                                                              agATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGa          >  2:1058575/1‑137 (MQ=255)                                                                                                                               gATATGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAg       >  1:638920/1‑139 (MQ=255)                                                                                                                                   tGCCGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGtt   >  2:1156168/1‑139 (MQ=255)                                                                                                                                     ccGTTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTc  <  1:239236/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAAT‑GTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTC  >  NZ_CP009273/4469704‑4469972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑