| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 369,445 | T→A | V41E (GTG→GAG) | dmpG → | 4‑hydroxy‑2‑oxovalerate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 369,445 | 0 | T | A | 92.2% | 232.8 / 8.6 | 64 | V41E (GTG→GAG) | dmpG | 4‑hydroxy‑2‑oxovalerate aldolase |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base A (24/35); total (26/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01 | |||||||||||
GCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACGTTGTT > NZ_CP009273/369310‑369580 | gCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGa < 2:28052/139‑1 (MQ=255)gCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGa < 2:683164/139‑1 (MQ=255) gCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTg > 1:1053374/1‑138 (MQ=255) cGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGaa < 2:635666/139‑1 (MQ=255) ggCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAg < 2:1058569/139‑1 (MQ=255) aaaaaCTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGa < 2:1113220/139‑1 (MQ=255) aaaaaCTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGa < 1:662314/139‑1 (MQ=255) aaaaaCTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGa < 1:621365/139‑1 (MQ=255) ttATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTg > 1:353047/1‑139 (MQ=255) tataTCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTg > 2:93901/1‑138 (MQ=255) tataTCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGATGGAAAACTTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTg > 1:1011035/1‑138 (MQ=255) atatCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGc < 1:754919/138‑1 (MQ=255) tatCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCa < 1:262460/138‑1 (MQ=255) tatCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCa < 1:928195/138‑1 (MQ=255) tatCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCa < 2:289665/138‑1 (MQ=255) tatCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCa < 2:187003/138‑1 (MQ=255) tatCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCa < 2:1117062/138‑1 (MQ=255) acaTTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCttt < 2:790778/138‑1 (MQ=255) acaTTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCttt < 2:983000/138‑1 (MQ=255) tGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGt > 1:618757/1‑139 (MQ=255) ggTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCg < 1:1248837/139‑1 (MQ=255) tATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgc > 1:657372/1‑139 (MQ=255) tGCACGCCATTCGTCATCAGTATTCGCTGGAAAACTTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgcg < 2:1011035/138‑1 (MQ=255) tGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgcg > 1:308301/1‑138 (MQ=255) tGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgcg > 1:1156066/1‑138 (MQ=255) ccATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAg < 2:1101292/139‑1 (MQ=255) ccATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAg < 2:1061062/139‑1 (MQ=255) ccATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAg < 2:999315/139‑1 (MQ=255) ccATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAg < 1:93901/139‑1 (MQ=255) cATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGc > 2:520730/1‑139 (MQ=255) aTTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGc > 1:959853/1‑90 (MQ=255) aTTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGc < 2:959853/90‑1 (MQ=255) aTTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGc > 1:1287214/1‑138 (MQ=255) aTTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGc > 1:216383/1‑138 (MQ=255) aTTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCg < 2:1142117/139‑1 (MQ=255) tCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAg < 2:766220/135‑1 (MQ=255) tCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAg > 1:766220/1‑135 (MQ=255) cGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGAc < 1:1061526/138‑1 (MQ=255) atcaGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGa < 2:308301/139‑1 (MQ=255) tcaGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGaa < 1:520730/139‑1 (MQ=255) caGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGa < 2:814507/64‑1 (MQ=255) caGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGa > 1:814507/1‑64 (MQ=255) ttCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgcgc > 1:1155612/1‑117 (MQ=255) ttCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgcgc < 2:1155612/117‑1 (MQ=255) ttCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGAtt > 2:25479/1‑139 (MQ=255) aaaaCGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCCCGGCGa < 2:91857/70‑1 (MQ=255) aaaaCGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGa > 1:91857/1‑70 (MQ=255) ccGTTCGCCAGATGGCCAAAGCACTGGACGATGCCCGCGAGGAGTCGATTGAAGGGGCCCACGGCGACGGTTTGTAAGGTTCCAGCTTTAACTGTGGTTGCGGCGCACATAGCGACCTTGAATGGATTGAAgcggcggc < 2:1044305/138‑1 (MQ=255) gTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGcggcggcgg < 2:63139/139‑1 (MQ=255) gTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGcggcggcgg < 2:618757/139‑1 (MQ=255) tCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCgg < 2:227597/99‑1 (MQ=255) tCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCgg > 1:227597/1‑99 (MQ=255) gCCAAAGCACTGGACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAg < 1:1081570/138‑1 (MQ=255) gACGATGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATcg > 2:681880/1‑139 (MQ=255) tGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACg > 1:1272466/1‑139 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGtt > 2:1158333/1‑50 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGtt < 1:1158333/50‑1 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgcg > 1:180970/1‑74 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGgcg < 2:180970/74‑1 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACg > 2:499360/1‑139 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACg < 2:375669/139‑1 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACg > 2:792882/1‑139 (MQ=255) tGCCCGCGAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACg > 1:921010/1‑139 (MQ=255) gcgAGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACgttgtt > 2:134082/1‑139 (MQ=255) | GCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTATTCGCTGGAAAACGTTCGCCAGATTGCCAAAGCACTGGACGATGCCCGCGTGGATTCGATTGAAGTGGCCCACGGCGACGGTTTGCAAGGTTCCAGCTTTAACTATGGTTTCGGCGCACATAGCGACCTTGAATGGATTGAAGCGGCGGCGGATGTGGTGAAGCACGCCAAAATCGCGACGTTGTT > NZ_CP009273/369310‑369580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |