| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,534,382 | C→T | P88S (CCT→TCT) | fimC → | type 1 fimbria chaperone FimC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,534,382 | 0 | C | T | 96.7% | 220.4 / ‑2.0 | 61 | P88S (CCT→TCT) | fimC | type 1 fimbria chaperone FimC |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (30/29); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
GACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGTCAATGGATAAATCA > NZ_CP009273/4534246‑4534516 | gACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGtct > 2:897206/1‑139 (MQ=255) aCTCGCGTAATTTATCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCG‑TTTATCGTGACGtctcc > 2:359232/1‑139 (MQ=255) gcgTAATTTATCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGtctcct < 1:1118053/137‑1 (MQ=255) tCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGAt > 1:798172/1‑138 (MQ=255) cGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGaa < 2:883636/139‑1 (MQ=255) aGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAggg > 1:1226755/1‑138 (MQ=255) gggCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGaa < 2:1169240/139‑1 (MQ=255) ggCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGaa > 1:555715/1‑138 (MQ=255) ggCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGaa > 1:1075905/1‑138 (MQ=255) ggCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGaa > 1:888743/1‑138 (MQ=255) aaCAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAaga < 1:637268/139‑1 (MQ=255) cAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAgaga < 2:452386/139‑1 (MQ=255) cAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAgaga < 2:953280/139‑1 (MQ=255) aaGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGaa < 2:762037/139‑1 (MQ=255) cTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGaa > 2:104479/1‑131 (MQ=255) cTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGaa < 1:104479/131‑1 (MQ=255) ataatGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGc < 2:1208992/139‑1 (MQ=255) atgaAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACaaa > 2:1210994/1‑139 (MQ=255) aaTAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAAc > 1:83570/1‑138 (MQ=255) aTTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAGGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCca > 2:762090/1‑137 (MQ=255) aTTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCca > 2:1023975/1‑137 (MQ=255) tttAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCcaca < 1:1210994/138‑1 (MQ=255) tttAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCcac < 2:1093562/137‑1 (MQ=255) aTTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGAcc > 1:827908/1‑139 (MQ=255) aaTCATGGGTGGAAAATGCCGATGGTGTAAGGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg < 1:762090/138‑1 (MQ=255) aaTCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg < 2:1238202/138‑1 (MQ=255) aaTCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg < 2:187525/138‑1 (MQ=255) aaTCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg < 2:902201/138‑1 (MQ=255) tCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGc > 2:487757/1‑107 (MQ=255) tCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGc < 1:487757/107‑1 (MQ=255) tCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGa > 2:560707/1‑137 (MQ=255) aTGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGt < 1:952967/139‑1 (MQ=255) gAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCt > 2:300023/1‑139 (MQ=255) tGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGAt > 2:1071384/1‑138 (MQ=255) tGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATg < 1:1071384/139‑1 (MQ=255) tGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATAc < 2:1270498/69‑1 (MQ=255) tGGTGTAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATAc > 1:1270498/1‑69 (MQ=255) tgtAAAGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTaaa < 1:897206/139‑1 (MQ=255) aGGATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGAt < 2:440521/139‑1 (MQ=255) gATGGTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTc < 1:291897/139‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGCGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGt > 2:767533/1‑139 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATAc < 2:578167/56‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATAc > 1:578167/1‑56 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGc < 2:884306/74‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGc > 1:884306/1‑74 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAAt > 2:1116732/1‑88 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAAt < 1:1116732/88‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAAt > 1:544974/1‑88 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAAt < 2:544974/88‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCcac > 1:178862/1‑94 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCcac < 2:178862/94‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg > 2:1023725/1‑103 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg > 1:1011374/1‑103 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg < 2:1011374/103‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCggg < 1:1023725/103‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGAtt > 2:393969/1‑135 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGAtt < 1:393969/135‑1 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGt > 1:672455/1‑139 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGt > 2:84507/1‑139 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGt > 1:499382/1‑139 (MQ=255) ggTCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGt > 1:119043/1‑139 (MQ=255) tCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTc > 1:880276/1‑134 (MQ=255) tCGTTTTATCGTGACGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTc < 2:880276/134‑1 (MQ=255) aCGTCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGTCAATGGATAAATCa > 1:44058/1‑138 (MQ=255) | GACTCGCGTAATTTATCCGGCAGGGCAAAAACAAGAGCAACTTGCCGTGACAAATAATGATGAAAATAGTACCTATTTAATTCAATCATGGGTGGAAAATGCCGATGGTGTAAAGGATGGTCGTTTTATCGTGACGCCTCCTCTGTTTGCGATGAAGGGAAAAAAAGAGAATACCTTACGTATTCTTGATGCAACAAATAACCAATTGCCACAGGACCGGGAAAGTTTATTCTGGATGAACGTTAAAGCGATTCCGTCAATGGATAAATCA > NZ_CP009273/4534246‑4534516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |