Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	400,441	T→C	E220E (GAA→GAG)	proC ←	pyrroline‑5‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	400,441	0	T	C	100.0%	221.9 / NA	60	E220E (GAA→GAG)	proC	pyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

CGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGG  >  NZ_CP009273/400330‑400571                                                                                                                |                                                                                                                                   cGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  1:7726/1‑139 (MQ=255) cGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  2:17121/1‑139 (MQ=255) cGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  1:582357/1‑139 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:84248/138‑1 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:821812/138‑1 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:1258338/138‑1 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  2:734376/138‑1 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:17121/138‑1 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  1:234798/1‑138 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  2:424005/138‑1 (MQ=255)  gTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  2:511592/138‑1 (MQ=255)         cTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  2:292147/131‑1 (MQ=255)         cTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  1:292147/1‑131 (MQ=255)         cTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCAt                                                                                                 >  1:182822/1‑139 (MQ=255)            cGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCAtt                                                                                                <  2:1029234/137‑1 (MQ=255)                  cTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTg                                                                                        <  1:560875/139‑1 (MQ=255)                             aaGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  1:526003/1‑111 (MQ=255)                             aaGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  2:526003/111‑1 (MQ=255)                             aaGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAAtt                                                                             >  2:632527/1‑139 (MQ=255)                                                cgcgTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:714834/92‑1 (MQ=255)                                                cgcgTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  2:714834/1‑92 (MQ=255)                                                cgcgTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGgc                                                                <  2:270052/133‑1 (MQ=255)                                                cgcgTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGgc                                                                >  1:270052/1‑133 (MQ=255)                                                     aCCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:1247710/87‑1 (MQ=255)                                                     aCCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  2:1247710/1‑87 (MQ=255)                                                       cGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCg                                                   >  1:659628/1‑139 (MQ=255)                                                       cGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCg                                                   <  1:632527/139‑1 (MQ=255)                                                        gCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGc                                                  >  1:268138/1‑139 (MQ=255)                                                                    gtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCgg                                                                                                        >  1:813354/1‑73 (MQ=255)                                                                    gtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCgg                                                                                                        <  2:813354/73‑1 (MQ=255)                                                                    gtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGa                                                                                                       >  2:834369/1‑74 (MQ=255)                                                                    gtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGa                                                                                                       <  1:834369/74‑1 (MQ=255)                                                                    gtgCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCAcggcgg                                      >  1:965891/1‑139 (MQ=255)                                                                       ccTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGt                                   >  2:3590/1‑139 (MQ=255)                                                                            cGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGcc                              <  2:1115900/139‑1 (MQ=255)                                                                                       ccATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGTGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGAt                   <  2:498195/139‑1 (MQ=255)                                                                                       ccATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGAt                   <  2:20772/139‑1 (MQ=255)                                                                                       ccATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGAt                   <  2:572395/139‑1 (MQ=255)                                                                                        cATATCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATa                  <  2:7726/139‑1 (MQ=255)                                                                                         atatCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATaa                 <  2:651599/139‑1 (MQ=255)                                                                                           atCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:397041/49‑1 (MQ=255)                                                                                           atCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  2:397041/1‑49 (MQ=255)                                                                                           atCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCg                                                   <  2:482593/103‑1 (MQ=255)                                                                                           atCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCg                                                   >  1:482593/1‑103 (MQ=255)                                                                                           atCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATaaa                >  1:253471/1‑138 (MQ=255)                                                                                           atCTTTCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATaaa                >  1:139043/1‑138 (MQ=255)                                                                                                tCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATaaa          >  2:955561/1‑139 (MQ=255)                                                                                                tCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATaaa          >  1:659035/1‑139 (MQ=255)                                                                                                tCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATaaa          >  1:732226/1‑139 (MQ=255)                                                                                                tCAGTGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATaa           <  2:732226/138‑1 (MQ=255)                                                                                                    tGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATc                                                      <  2:27073/91‑1 (MQ=255)                                                                                                    tGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATc                                                      >  1:27073/1‑91 (MQ=255)                                                                                                    tGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACg      >  1:434349/1‑139 (MQ=255)                                                                                                    tGCCCCCGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACg      >  2:674587/1‑139 (MQ=255)                                                                                                      cccccGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         >  2:1006668/1‑38 (MQ=255)                                                                                                      cccccGGATGCTCTCCCGTTTCCAGCACCATTTTTGCg                                                                                                         <  1:1006668/38‑1 (MQ=255)                                                                                                      cccccGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACg      >  2:976141/1‑137 (MQ=255)                                                                                                        cccGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAgg  >  1:406208/1‑139 (MQ=255)                                                                                                          cGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATa            <  2:1214741/127‑1 (MQ=255)                                                                                                          cGGATGCTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATa            >  1:1214741/1‑127 (MQ=255)                                                                                                                |                                                                                                                                   CGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTCCAGCACCATTTTTGCGGAACCCATTACCGCCTGAGCGGCAAATTTATACGCCTGGGCGCGTGGCATCCCGCCCAGCACGGCGGCGTCGGCCATCGCTTCGATAAACATAAATACGTAGG  >  NZ_CP009273/400330‑400571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑