Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	412,148	G→A	V87V (GTC→GTT)	sbcD ←	exonuclease subunit SbcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	412,148	0	G	A	97.2%	255.0 / ‑2.9	71	V87V (GTC→GTT)	sbcD	exonuclease subunit SbcD
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (40/29);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCACCGGCAACAATA  >  NZ_CP009273/412014‑412280                                                                                                                                       |                                                                                                                                     aaaaaCGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAAcc                                                                                                                                   >  1:526466/1‑138 (MQ=255) aaaaaCGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAAcc                                                                                                                                   >  1:371472/1‑138 (MQ=255)      cGGAATGTGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCa                                                                                                                             <  2:734261/139‑1 (MQ=255)       ggAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAAc                                                                                                                                    <  1:1031882/131‑1 (MQ=255)       ggAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAAc                                                                                                                                    >  2:1031882/1‑131 (MQ=255)                cacaGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTg                                                                                                                   <  2:7119/139‑1 (MQ=255)                cacaGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTg                                                                                                                   <  2:461612/139‑1 (MQ=255)                   aGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGc                                                                                                                  >  2:956999/1‑137 (MQ=255)                       cTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTac                                                                                                            <  2:75648/139‑1 (MQ=255)                        tGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTac                                                                                                            >  2:192907/1‑138 (MQ=255)                        tGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTac                                                                                                            >  2:1138372/1‑138 (MQ=255)                        tGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTac                                                                                                            >  2:1251995/1‑138 (MQ=255)                               ggCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGAt                                                                                                    >  2:519984/1‑139 (MQ=255)                               ggCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGAt                                                                                                    >  1:568649/1‑139 (MQ=255)                               ggCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGAt                                                                                                    >  2:620556/1‑139 (MQ=255)                               ggCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGAt                                                                                                    >  1:449428/1‑139 (MQ=255)                                    cccGTCGCGACGAGGCAAGATTTGCGGCGCATGTTCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAg                                                                                               >  1:65370/1‑139 (MQ=255)                                    cccGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGttt                                                                                                                       <  2:498917/115‑1 (MQ=255)                                    cccGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGttt                                                                                                                       >  1:498917/1‑115 (MQ=255)                                     ccGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCt                                                                                                                    >  2:1002689/1‑117 (MQ=255)                                     ccGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCt                                                                                                                    <  1:1002689/117‑1 (MQ=255)                                                  gCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAg                                                                                                      <  2:1285392/118‑1 (MQ=255)                                                  gCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAg                                                                                                      >  1:1285392/1‑118 (MQ=255)                                                           gcggcgCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGacaa                                                                         >  2:383256/1‑138 (MQ=255)                                                               cgcATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                                                    <  1:242106/139‑1 (MQ=255)                                                               cgcATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                                                    <  2:526466/139‑1 (MQ=255)                                                                 cATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACg                                                                  <  1:956999/139‑1 (MQ=255)                                                                              ggCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAAc                                                                                                                                    <  1:285685/60‑1 (MQ=255)                                                                              ggCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAAc                                                                                                                                    >  2:285685/1‑60 (MQ=255)                                                                              ggCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTac                                                                                                            <  1:498416/84‑1 (MQ=255)                                                                              ggCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTac                                                                                                            >  2:498416/1‑84 (MQ=255)                                                                              ggCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGAcaacaa                                                                      >  1:1176057/1‑122 (MQ=255)                                                                              ggCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGAcaacaa                                                                      <  2:1176057/122‑1 (MQ=255)                                                                               gCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGc                                                    >  1:1163522/1‑139 (MQ=255)                                                                               gCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGc                                                    <  1:1088087/139‑1 (MQ=255)                                                                                  aCCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTCTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCggg                                                 <  2:320929/139‑1 (MQ=255)                                                                                    cACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCg                                               >  1:954443/1‑139 (MQ=255)                                                                                         tagtaTTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACt                                          >  1:211461/1‑139 (MQ=255)                                                                                         tagtaTTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACt                                          >  2:990093/1‑139 (MQ=255)                                                                                                 aGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGcc                                                                                                                 >  1:37176/1‑60 (MQ=255)                                                                                                 aGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGcc                                                                                                                 <  2:37176/60‑1 (MQ=255)                                                                                                 aGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGggcggc                                   >  2:264448/1‑138 (MQ=255)                                                                                                 aGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGggcggc                                   >  1:781761/1‑138 (MQ=255)                                                                                                 aGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGggcggc                                   >  1:1256947/1‑138 (MQ=255)                                                                                                   gAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAg                                >  2:978261/1‑139 (MQ=255)                                                                                                   gAACGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAg                                >  1:442657/1‑139 (MQ=255)                                                                                                      cGCCATGATATCGCGCGATTCATTCAGGGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCg                             <  2:583532/139‑1 (MQ=255)                                                                                                      cGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGGGTCATGGTTTCCTGcc                                                                                                                 >  2:542631/1‑55 (MQ=255)                                                                                                      cGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGcc                                                                                                                 <  1:542631/55‑1 (MQ=255)                                                                                                      cGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                                                    >  2:730788/1‑100 (MQ=255)                                                                                                      cGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                                                    <  1:730788/100‑1 (MQ=255)                                                                                                      cGCCATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCg                             <  1:383256/139‑1 (MQ=255)                                                                                                        ccATGATATCGCGCGATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCgg                            >  2:1214492/1‑138 (MQ=255)                                                                                                                 cgcgcgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGac                                                                           <  2:504704/82‑1 (MQ=255)                                                                                                                 cgcgcgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGac                                                                           >  1:504704/1‑82 (MQ=255)                                                                                                                 cgcgcgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGaaaa                   >  1:1138946/1‑138 (MQ=255)                                                                                                                 cgcgcgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGaaaa                   >  2:811378/1‑138 (MQ=255)                                                                                                                   cgcgATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACAt                >  2:377890/1‑139 (MQ=255)                                                                                                                   cgcgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACAt                >  1:630338/1‑139 (MQ=255)                                                                                                                   cgcgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACAt                >  1:1182277/1‑139 (MQ=255)                                                                                                                     cgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCa              <  1:879680/139‑1 (MQ=255)                                                                                                                     cgATTCATTCAGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCa              <  1:896722/139‑1 (MQ=255)                                                                                                                        ttcattcaGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                                                    <  1:1151/82‑1 (MQ=255)                                                                                                                        ttcattcaGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                                                    >  2:1151/1‑82 (MQ=255)                                                                                                                        ttcattcaGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCACCg           >  2:1158338/1‑139 (MQ=255)                                                                                                                           attcaGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCACCGGc         <  1:1138372/138‑1 (MQ=255)                                                                                                                           attcaGCGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCACCGGc         <  1:990093/138‑1 (MQ=255)                                                                                                                                 cGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCACCGGCAACaata  <  2:781761/139‑1 (MQ=255)                                                                                                                                 cGTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCACCGGCAACaat   >  2:751644/1‑138 (MQ=255)                                                                                                                                  gTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc                               >  1:395894/1‑109 (MQ=255)                                                                                                                                  gTGGCAACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGc                               <  2:395894/109‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     AAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGAGCCGGTATCGAAAACATCACCGGCAACAATA  >  NZ_CP009273/412014‑412280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑