Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	443,081	C→T	L27L (CTG→CTA)	cyoE ←	heme o synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	443,081	0	C	T	98.4%	231.2 / ‑7.2	62	L27L (CTG→CTA)	cyoE	heme o synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (31/30);  minor base A (0/1);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCA  >  NZ_CP009273/442950‑443205                                                                                                                                     |                                                                                                                              gTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAAt                                                                                                                         <  1:922567/138‑1 (MQ=255)      ttCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATccccc                                                                                                                    >  1:416761/1‑138 (MQ=255)        ccATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCaa                                                                                                                  <  1:889673/138‑1 (MQ=255)          aTCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCa                                                                                                               <  2:16520/139‑1 (MQ=255)               tCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCAcc                                                                                                             >  2:1147370/1‑136 (MQ=255)                      atatCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGt                                                                                                   >  1:1258088/1‑139 (MQ=255)                             tGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGt                                                                                                   >  1:1083889/1‑132 (MQ=255)                             tGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGt                                                                                                   <  2:1083889/132‑1 (MQ=255)                                 gATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAgatg                                                                                        <  1:120405/139‑1 (MQ=255)                                    gTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGatgatg                                                                                     >  2:510731/1‑139 (MQ=255)                                       ggtgttAAACACACACCACGACGCCACATCCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGAGAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCCATCAACGCGATCATGTTGCCAAAGATGATGCC‑t                                                                                 >  2:284321/3‑139 (MQ=255)                                         tgttAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑Tgg                                                                               >  2:1021518/1‑139 (MQ=255)                                         tgttAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑Tgg                                                                               >  1:494491/1‑139 (MQ=255)                                             aaaCACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTc                                                                           >  2:183955/1‑139 (MQ=255)                                                 acacaACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTa                                                                       <  1:737598/139‑1 (MQ=255)                                                   acaACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACt                                                                     <  2:305593/139‑1 (MQ=255)                                                     aaCCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTg                                                                   >  2:338116/1‑139 (MQ=255)                                                      aCCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGc                                                                  <  2:494491/139‑1 (MQ=255)                                                          gACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGtt                                                                        >  2:805017/1‑129 (MQ=255)                                                          gACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGtt                                                                        <  1:805017/129‑1 (MQ=255)                                                          gACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGt                                                              <  2:33386/139‑1 (MQ=255)                                                          gACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGt                                                              <  2:927716/139‑1 (MQ=255)                                                          gACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGt                                                              <  1:1147370/139‑1 (MQ=255)                                                          gACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGt                                                              <  2:711804/139‑1 (MQ=255)                                                           aCGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTa                                                             >  1:124932/1‑139 (MQ=255)                                                           aCGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTa                                                             <  1:1185268/139‑1 (MQ=255)                                                               cacaACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGc                                                         >  2:360471/1‑139 (MQ=255)                                                                 caACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGt                                                              >  1:1098799/1‑132 (MQ=255)                                                                 caACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGt                                                              <  2:1098799/132‑1 (MQ=255)                                                                 caACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTa                                                             <  1:752455/133‑1 (MQ=255)                                                                 caACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTa                                                             >  2:752455/1‑133 (MQ=255)                                                                  aaCCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTa                                                      <  2:1174944/139‑1 (MQ=255)                                                                  aaCCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTa                                                      <  1:238610/139‑1 (MQ=255)                                                                  aaCCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTa                                                      <  1:518570/139‑1 (MQ=255)                                                                  aaCCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTa                                                      <  2:805609/139‑1 (MQ=255)                                                                    ccAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTaaa                                                    <  2:117811/139‑1 (MQ=255)                                                                       gTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAAcat                                                 >  1:345031/1‑139 (MQ=255)                                                                            acCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAgatg                                                                                        >  2:1098454/1‑96 (MQ=255)                                                                            acCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAgatg                                                                                        <  1:1098454/96‑1 (MQ=255)                                                                                       cGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGctct                                  >  2:507329/1‑138 (MQ=255)                                                                                        gtgtAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCcagc                                                                                                                               <  1:1039277/45‑1 (MQ=255)                                                                                        gtgtAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCcagc                                                                                                                               >  2:1039277/1‑45 (MQ=255)                                                                                        gtgtAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGctct                                  >  1:627824/1‑137 (MQ=255)                                                                                        gtgtAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGctct                                  >  2:494319/1‑137 (MQ=255)                                                                                           tAGAAAAAAAGGGATAAATCATGGCTGCCTTTT‑GAGCCAGGTAGGACCCCCCCAATCCCCGAGATCGGGTTGCAAAAGATGTTGCT‑TGTTTTCGTTCTTTGCAGTTTTTTTTTAAACATCATAACCGCCGCTCTTAGTg                             <  2:1258088/139‑1 (MQ=255)                                                                                                     ggggATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGc                                                                  >  2:218039/1‑92 (MQ=255)                                                                                                     ggggATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGc                                                                  <  1:218039/92‑1 (MQ=255)                                                                                                     ggggATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtg                   >  2:897346/1‑139 (MQ=255)                                                                                                     ggggATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtg                   >  1:466784/1‑139 (MQ=255)                                                                                                     ggggATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtg                   >  1:170162/1‑139 (MQ=255)                                                                                                      gggATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtgt                  >  2:783645/1‑139 (MQ=255)                                                                                                      gggATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtgt                  >  2:1105956/1‑139 (MQ=255)                                                                                                      gggAAAAACAATGCCTCCCTTTGGGGGCCAGAAGGAACCCCCAAACCACCGAAATCGGGTTCCAAAAGATG‑TGCCTTGTTTTCTTTCTTTGAAGTTTTTTTTAAAACATAATAACCGCCGCTCTTAGTGCATCATCAtg                   <  2:940469/139‑1 (MQ=255)                                                                                                        gATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTa                                                             >  1:836773/1‑94 (MQ=255)                                                                                                        gATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTa                                                             <  2:836773/94‑1 (MQ=255)                                                                                                        gATAATCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCAtgttg                >  1:642243/1‑139 (MQ=255)                                                                                                           aaTCAATGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAg             >  1:619529/1‑139 (MQ=255)                                                                                                              catggctccCCTTG‑GAGGCCAGTAGGACCCCCCCAACCCCCGATATCAGGTGGCAAAAGATGATGCC‑TGTTTTCTTTCTTGGCAGTTTTTTCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTg          <  1:783645/135‑1 (MQ=255)                                                                                                              cAATGCTCCCTTTG‑GAGGCCAGTAGGAACCCCCCAATCCCCGAGATCGGGTTGCAAAAGATGATCCT‑TGTTTTCTTTTTTGGCGGGTTTTGCTAAAACAACATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTg          <  1:183955/139‑1 (MQ=255)                                                                                                                aTGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTa                                                      >  2:386526/1‑93 (MQ=255)                                                                                                                aTGCTGCCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTa                                                      <  1:386526/93‑1 (MQ=255)                                                                                                                   ctgcCCTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCACCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGt      <  1:943265/138‑1 (MQ=255)                                                                                                                   ctgcCCTTT‑GAGGCCAGTAGGAACCCCCCAACCCCCGAGATCAGTTTGCAAAAGATGATGCC‑TGTTTCCTTTACTTGCGGGTATTGTTAAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGt      <  1:1137314/138‑1 (MQ=255)                                                                                                                      cccTTT‑GAGGCCAGTAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCca  >  1:287134/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                              GTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAACCAGTGACACCCCAACCAGCGTGTAGATAAACAGGGGATAATCAATGCTGCCCTTT‑GAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCC‑TGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCA  >  NZ_CP009273/442950‑443205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑