Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	449,442	A→G	V61A (GTC→GCC)	yajG ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	449,442	0	A	G	98.6%	248.7 / ‑3.8	71	V61A (GTC→GCC)	yajG	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (32/38);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

ATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTG  >  NZ_CP009273/449307‑449577                                                                                                                                        |                                                                                                                                        atgatTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCtt                                                                                                                                      >  2:59885/1‑139 (MQ=255)   gatTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCtttt                                                                                                                                    >  1:712382/1‑139 (MQ=255)       tGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGcc                                                                                                                                 <  2:1193845/138‑1 (MQ=255)       tGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGcc                                                                                                                                 <  1:1226047/138‑1 (MQ=255)                aCCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGAt                                                                                                                       <  1:948855/139‑1 (MQ=255)                  cGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCg                                                                                                                     <  1:848329/139‑1 (MQ=255)                  cGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCg                                                                                                                     <  1:206222/139‑1 (MQ=255)                    ggCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCgg                                                                                                                    >  2:1186062/1‑138 (MQ=255)                     gCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTa                                                                                                                  <  2:881366/139‑1 (MQ=255)                          tCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCt                                                                                                              >  1:344526/1‑138 (MQ=255)                            ggACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGAt                                                                                                           <  1:36900/139‑1 (MQ=255)                                caaccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGc                                                                                                       <  1:146487/139‑1 (MQ=255)                                  accaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCtt                                                                                                                                      <  2:564511/106‑1 (MQ=255)                                  accaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCtt                                                                                                                                      >  1:564511/1‑106 (MQ=255)                                  accaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCAc                                                                                                     >  2:1124093/1‑139 (MQ=255)                                   ccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGAt                                                                                                                       >  2:484833/1‑120 (MQ=255)                                   ccaTGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGAt                                                                                                                       <  1:484833/120‑1 (MQ=255)                                      tGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCAtt                                                                                                 <  1:59885/139‑1 (MQ=255)                                      tGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCAtt                                                                                                 <  2:712382/139‑1 (MQ=255)                                        tAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTaa                                                                                               >  2:977377/1‑139 (MQ=255)                                        tAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTa                                                                                                <  1:1124093/138‑1 (MQ=255)                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCg                                                                                                                     <  2:525378/111‑1 (MQ=255)                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCg                                                                                                                     >  1:525378/1‑111 (MQ=255)                                              cgcgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTa                                                                                         >  1:1014232/1‑139 (MQ=255)                                              cgcgcgGTCATATGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGCTTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTa                                                                                         >  1:200745/1‑139 (MQ=255)                                                cgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGTTTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACg                                                                                       >  2:133635/1‑139 (MQ=255)                                                cgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCgg                                                                                                        <  2:284172/122‑1 (MQ=255)                                                cgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCgg                                                                                                        >  1:284172/1‑122 (MQ=255)                                                cgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACg                                                                                       >  1:275386/1‑139 (MQ=255)                                                cgcgGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACg                                                                                       >  1:63899/1‑139 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGTTCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  1:1106923/138‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  1:817340/138‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  2:811733/138‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATc                                                                           <  1:133635/139‑1 (MQ=255)                                                            tttttCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCAt                                                                            <  2:1025733/138‑1 (MQ=255)                                                                 ccAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGc                                                                       <  2:1026085/138‑1 (MQ=255)                                                                   aGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAAt                                                                                              >  2:275011/1‑113 (MQ=255)                                                                   aGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAAt                                                                                              <  1:275011/113‑1 (MQ=255)                                                                          cttGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCctg                                                             >  2:429403/1‑139 (MQ=255)                                                                              cagcagGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCctgctg                                                          <  2:88674/138‑1 (MQ=255)                                                                                    gAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAgt                                                   >  1:1092419/1‑139 (MQ=255)                                                                                    gAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAgt                                                   >  1:300170/1‑139 (MQ=255)                                                                                    gAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAgt                                                   >  1:1051468/1‑139 (MQ=255)                                                                                    gAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATAGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAgt                                                   >  1:476650/1‑139 (MQ=255)                                                                                       aCGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATAGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTa                                                <  2:476650/139‑1 (MQ=255)                                                                                           aGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAAtcg                                            >  1:903562/1‑139 (MQ=255)                                                                                                  gggAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGa                                     <  1:366544/139‑1 (MQ=255)                                                                                                  gggAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGa                                     <  1:592898/139‑1 (MQ=255)                                                                                                  gggAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTAGGATCCTGCTGTGGCAGTGTAATCGTCGGGGa                                     <  1:1108539/139‑1 (MQ=255)                                                                                                   ggAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTaa                                               <  2:833449/128‑1 (MQ=255)                                                                                                   ggAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTaa                                               >  1:833449/1‑128 (MQ=255)                                                                                                       gCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCgg                                                                                                        <  1:254100/67‑1 (MQ=255)                                                                                                       gCGGTCAGGGTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCgg                                                                                                        >  2:254100/1‑67 (MQ=255)                                                                                                          gTCAGGGGAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCaa                             >  1:581755/1‑139 (MQ=255)                                                                                                               ggTAACGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAgtt                        >  2:1238865/1‑139 (MQ=255)                                                                                                                    cGATTTGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTgg                   >  2:91650/1‑139 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGAt                                                                 >  2:453107/1‑88 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGAt                                                                 <  1:453107/88‑1 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGtt              >  1:1093241/1‑139 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGtt              >  1:932886/1‑139 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGtt              >  1:673395/1‑139 (MQ=255)                                                                                                                         tGATTATCGCGGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGt               <  2:1022536/138‑1 (MQ=255)                                                                                                                                cgcgGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCa       >  1:212920/1‑139 (MQ=255)                                                                                                                                 gcgGGTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCa       <  2:788580/138‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  2:344526/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  2:212920/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  2:1023626/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  1:977377/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  1:867920/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  2:866661/139‑1 (MQ=255)                                                                                                                                     gTGGCTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTg  <  1:749916/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATCGGTACGCTGATCGGCACCATTAATGCTTACGGTGACGCCCATCAGGCTTGGATCCTGCTGTGGCAGTGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTG  >  NZ_CP009273/449307‑449577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑