Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	547,771	T→C	T94A (ACT→GCT)	purK ←	5‑(carboxyamino)imidazole ribonucleotide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	547,771	0	T	C	100.0%	205.5 / NA	56	T94A (ACT→GCT)	purK	5‑(carboxyamino)imidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/25);  total (31/25)

GACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCT  >  NZ_CP009273/547660‑547905                                                                                                                |                                                                                                                                       gACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAgct                                                                                                              <  2:1047617/138‑1 (MQ=255)    cgcgACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCtt                                                                                                          <  1:220218/139‑1 (MQ=255)    cgcgACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCtt                                                                                                          <  2:282373/139‑1 (MQ=255)              aTAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAGACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGa                                                                                                <  1:848076/139‑1 (MQ=255)                  ccaccaGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGt                                                                                            <  2:870393/139‑1 (MQ=255)                      caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAg                                                                                                                                  <  2:961536/97‑1 (MQ=255)                      caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAg                                                                                                                                  >  1:961536/1‑97 (MQ=255)                      caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                         >  2:117925/1‑138 (MQ=255)                      caGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAg                                                                                         >  1:807718/1‑138 (MQ=255)                        gTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaa                                                                                      >  1:40971/1‑139 (MQ=255)                              cGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCACTAATCg                                                                                >  1:454711/1‑139 (MQ=255)                              cGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCg                                                                                >  1:1195461/1‑139 (MQ=255)                                   aaCAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGaa                                                                            <  1:1089883/138‑1 (MQ=255)                                   aaCAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGaa                                                                            >  2:517401/1‑138 (MQ=255)                                   aaCAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGaa                                                                            >  2:4898/1‑138 (MQ=255)                                   aaCAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGaa                                                                            >  1:366888/1‑138 (MQ=255)                                   aaCAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGa                                                                             <  2:366888/137‑1 (MQ=255)                                         cGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCtt                                                                                                          <  2:290097/102‑1 (MQ=255)                                         cGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCtt                                                                                                          >  1:290097/1‑102 (MQ=255)                                                   ccTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACaa                                                           <  2:474483/139‑1 (MQ=255)                                                            tCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGAt                                                  >  1:1043278/1‑139 (MQ=255)                                                                     gCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAg                                                                                                                <  1:569311/68‑1 (MQ=255)                                                                     gCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAg                                                                                                                >  2:569311/1‑68 (MQ=255)                                                                      cAGGCTACTCGCTGCGTTGGGCAAGTTACTGCCACGGGGCAGCCGGCAGGTGGGGCTTATCGAAAAGCTGCTTCTGAGACAGACGGTCAGCGAGAATCGAGAACACATCGCGGTTCACAAAGGCCGGGTGGCGGGCCAg                                        >  2:27875/1‑139 (MQ=255)                                                                      cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACgg                                                                                             >  1:1226954/1‑86 (MQ=255)                                                                      cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACgg                                                                                             <  2:1226954/86‑1 (MQ=255)                                                                      cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                        >  1:883550/1‑139 (MQ=255)                                                                      cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                        >  2:675971/1‑139 (MQ=255)                                                                      cAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAg                                        >  2:973600/1‑139 (MQ=255)                                                                         gCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcaca                                                            <  2:647083/116‑1 (MQ=255)                                                                         gCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcaca                                                            >  1:365906/1‑116 (MQ=255)                                                                         gCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcaca                                                            <  2:365906/116‑1 (MQ=255)                                                                         gCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTcaca                                                            >  1:647083/1‑116 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaa                                                                                      <  2:1196044/85‑1 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCaa                                                                                      >  1:1196044/1‑85 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGa                                                   <  2:673146/120‑1 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGa                                                   >  1:673146/1‑120 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGgcgcgc                                           <  2:622371/128‑1 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGgcgcgc                                           >  1:622371/1‑128 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCggg                                >  2:766788/1‑139 (MQ=255)                                                                              tCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCggg                                >  1:843671/1‑139 (MQ=255)                                                                                   gCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGCACTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCCCAAAGGCCGGCTGGCGCGCCAGCTCGCGGGATAAt                           >  2:613045/1‑139 (MQ=255)                                                                                   gCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTcg                                    >  2:1193024/1‑130 (MQ=255)                                                                                   gCGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTcg                                    <  1:1193024/130‑1 (MQ=255)                                                                                    cGTTCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATg                          >  2:965201/1‑139 (MQ=255)                                                                                      ttCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACgg                                                                                             <  2:33126/70‑1 (MQ=255)                                                                                      ttCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACgg                                                                                             >  1:33126/1‑70 (MQ=255)                                                                                      ttCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACg                                                                                              <  2:37782/69‑1 (MQ=255)                                                                                      ttCGGCAAGTAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACg                                                                                              >  1:37782/1‑69 (MQ=255)                                                                                               tAACTGCCACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGc               <  1:517401/139‑1 (MQ=255)                                                                                                     ccACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTcg                                    <  2:392170/112‑1 (MQ=255)                                                                                                     ccACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTcg                                    >  1:392170/1‑112 (MQ=255)                                                                                                     ccACGGTGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCg          <  1:282720/138‑1 (MQ=255)                                                                                                           tGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACgg                                                                                             >  2:834621/1‑49 (MQ=255)                                                                                                           tGCAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACgg                                                                                             <  1:834621/49‑1 (MQ=255)                                                                                                             cAGCCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAtct  <  2:40971/138‑1 (MQ=255)                                                                                                                |                                                                                                                                       GACCGCGACCGTCATAACCACCAGTGCGACGCTTAACAATCGCCAGCTCACCTAAACGATCAAACACCGCAGGCCACTCGCTGCGTTCGGCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCT  >  NZ_CP009273/547660‑547905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑