Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	547,953	T→C	D33G (GAC→GGC)	purK ←	5‑(carboxyamino)imidazole ribonucleotide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	547,953	0	T	C	100.0%	132.7 / NA	38	D33G (GAC→GGC)	purK	5‑(carboxyamino)imidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/21);  total (17/21)

GAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGTCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCGGGTTTTCCAGCACTTCGT  >  NZ_CP009273/547829‑548087                                                                                                                             |                                                                                                                                       gAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGc                                                                                                                          >  1:47259/1‑139 (MQ=255)    cacaTCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGc                                                                                                                          >  2:681828/1‑136 (MQ=255)       aTCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGa                                                                                                                      >  1:185875/1‑137 (MQ=255)       aTCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGa                                                                                                                      >  2:739919/1‑137 (MQ=255)        tCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAg                                                                                                                   <  1:191390/139‑1 (MQ=255)        tCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAg                                                                                                                   <  1:739919/139‑1 (MQ=255)          gcgGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCa                                                                                                                 <  2:883550/139‑1 (MQ=255)          gcgGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCa                                                                                                                 <  2:477774/139‑1 (MQ=255)                cacaAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGcc                                                                                                            >  2:1026196/1‑138 (MQ=255)                   aaaGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAAc                                                                                                        >  1:540232/1‑139 (MQ=255)                     aGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACgg                                                                                                      >  2:331472/1‑139 (MQ=255)                        ccGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTc                                                                                                   >  1:1233693/1‑139 (MQ=255)                                cgcgcCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCgc                                                                                                 >  1:686757/1‑133 (MQ=255)                                cgcgcCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCgc                                                                                                 <  2:686757/133‑1 (MQ=255)                                      aGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACgca                                                                                     <  1:22899/139‑1 (MQ=255)                                           gcgGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATa                                                                                <  2:696513/139‑1 (MQ=255)                                           gcgGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATa                                                                                <  2:185875/139‑1 (MQ=255)                                           gcgGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGATGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATa                                                                                <  1:965201/139‑1 (MQ=255)                                                                      ttCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACg                                                     <  1:766788/139‑1 (MQ=255)                                                                              cAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTg                                             >  1:27138/1‑139 (MQ=255)                                                                                 cGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCa                                                                                                                 <  1:837525/68‑1 (MQ=255)                                                                                 cGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCa                                                                                                                 >  2:837525/1‑68 (MQ=255)                                                                                 cGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGtt                                           <  1:377798/138‑1 (MQ=255)                                                                                 cGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGtt                                           >  2:377798/1‑138 (MQ=255)                                                                                     aaTCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATg                                      >  2:1090447/1‑139 (MQ=255)                                                                                      aTCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGc                                     >  2:179408/1‑139 (MQ=255)                                                                                          cGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCa                                 <  2:1195461/139‑1 (MQ=255)                                                                                               tgttgAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGc                                     <  1:1146720/130‑1 (MQ=255)                                                                                               tgttgAAAAGGCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGc                                     >  2:1146720/1‑130 (MQ=255)                                                                                                         gCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCggg                  <  2:27138/139‑1 (MQ=255)                                                                                                         gCACCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCggg                  <  2:1043278/139‑1 (MQ=255)                                                                                                           aCCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAg                                                                                                                   >  1:76089/1‑40 (MQ=255)                                                                                                           aCCGCCGCCGGTTCAGCGCCCAGCCCGACTGGCCAGACAg                                                                                                                   <  2:76089/40‑1 (MQ=255)                                                                                                                       tCAGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCGGGTTTTCCAGCACTtc    >  2:119425/1‑139 (MQ=255)                                                                                                                         aGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCGGGTTTTCCAGCACTtcgt  <  1:369124/139‑1 (MQ=255)                                                                                                                         aGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCGGGTTTTCCAGCACTtcg   <  2:540232/138‑1 (MQ=255)                                                                                                                         aGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCGGGTTTTCCAGCACTtcg   <  1:636015/138‑1 (MQ=255)                                                                                                                         aGCGCCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCGGGTTTTCCAGCACTtcg   <  1:1074947/138‑1 (MQ=255)                                                                                                                             |                                                                                                                                       GAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCACCGCCGCCGGTTCAGCGTCCAGCCCGACTGGCCAGACAGCAATGCCTAACGGTTCGCCTGCCTGACGCAGCATACGGCCTAACTGCCCGTTACCGAGGACGCAAACCTGTTTCATGCCGCACCTCGCGGGTCCGGGTTTTCCAGCACTTCGT  >  NZ_CP009273/547829‑548087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑