Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	585,704	T→A	D236V (GAT→GTT)	nfrA ←	bacteriophage adsorption protein NfrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	585,704	0	T	A	100.0%	214.5 / NA	53	D236V (GAT→GTT)	nfrA	bacteriophage adsorption protein NfrA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (26/27);  total (26/27)

TGGCTTATTTTCAATGAGATAATGCTGGAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGATCGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAGGTAGATTGCCCGT  >  NZ_CP009273/585577‑585832                                                                                                                                |                                                                                                                                 tGGCTTATTTTCAATGAGATAATGCTGGAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGc                                                                                                                       >  2:865274/1‑139 (MQ=255)    cTTATTTTCAATGAGATAATGCTGGAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCg                                                                                                                    >  1:540594/1‑139 (MQ=255)        ttttCAATGAGATAATGCTGGAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGc                                                                                                                  >  1:1275511/1‑137 (MQ=255)             aaTGAGATAATGCTGGAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCaagaag                                                                                                            <  1:865274/138‑1 (MQ=255)             aaTGAGATAATGCTGGAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCaagaag                                                                                                            <  1:525237/138‑1 (MQ=255)                            gAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGc                                                                                                                       <  2:1082595/112‑1 (MQ=255)                            gAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGc                                                                                                                       >  1:1082595/1‑112 (MQ=255)                            gAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCTAACCACTGa                                                                                            >  2:24407/1‑139 (MQ=255)                                  tGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGt                                                                                      >  2:1111431/1‑139 (MQ=255)                                        ttCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGc                                                                                 <  2:540594/138‑1 (MQ=255)                                               cGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCtt                                                                          >  1:5452/1‑138 (MQ=255)                                                 aTAAGCCAGCGCGGTTGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAAt                                                                       <  2:586047/139‑1 (MQ=255)                                                          cgcgGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCaagaag                                                                                                            <  2:936749/93‑1 (MQ=255)                                                          cgcgGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCaagaag                                                                                                            >  1:936749/1‑93 (MQ=255)                                                               tCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGa                                                                                            <  1:834666/104‑1 (MQ=255)                                                               tCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGa                                                                                            >  2:834666/1‑104 (MQ=255)                                                               tCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCg                                                         <  1:482240/139‑1 (MQ=255)                                                                 gcgTAAGTAATATATGACTGATGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTg                                                       <  2:612420/139‑1 (MQ=255)                                                                 gcgTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTg                                                       <  1:428880/139‑1 (MQ=255)                                                                   gtaagtaaTATATGACTGAGGATCGGTGAAGATCCGCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     <  2:199692/139‑1 (MQ=255)                                                                   gtaagtaaTATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     <  2:357292/139‑1 (MQ=255)                                                                    taagtaaTATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCtt                                                    >  1:520839/1‑139 (MQ=255)                                                                          atatatGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGt                                              <  2:395916/139‑1 (MQ=255)                                                                                 aCTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCg                                        >  1:191512/1‑138 (MQ=255)                                                                                   tGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     <  2:1283365/123‑1 (MQ=255)                                                                                   tGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     >  1:1283365/1‑123 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     <  2:557042/121‑1 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     <  1:1041556/121‑1 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     >  2:1041556/1‑121 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     >  1:557042/1‑121 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTAt                                     <  2:894312/137‑1 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTAt                                     <  2:1275511/137‑1 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTAt                                     <  1:36988/137‑1 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTAt                                     <  1:441987/137‑1 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTAt                                     <  1:601352/137‑1 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCt                                   >  1:973921/1‑139 (MQ=255)                                                                                     aGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCAAGCTGCCCGGCAAGAAGCACGTCAAACAACTGACGGCGTTCTGCCGCGCTTATTGTGTTCTGCGGCCGTGCTTCTTTGTTTAGCGAATCt                                   >  1:683028/1‑139 (MQ=255)                                                                                       gATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGc                                 >  1:436744/1‑139 (MQ=255)                                                                                        aTCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGcc                                <  2:973921/139‑1 (MQ=255)                                                                                          cGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     <  1:735486/116‑1 (MQ=255)                                                                                          cGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCt                                                     >  2:735486/1‑116 (MQ=255)                                                                                          cGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTg                              >  2:272487/1‑139 (MQ=255)                                                                                            gTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGc                                                                                 >  2:7805/1‑86 (MQ=255)                                                                                            gTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGc                                                                                 <  1:7805/86‑1 (MQ=255)                                                                                            gTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTgg                             >  2:622273/1‑138 (MQ=255)                                                                                                    ccccTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGtata                                           >  2:323278/1‑116 (MQ=255)                                                                                                    ccccTGTGATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGtata                                           <  1:323278/116‑1 (MQ=255)                                                                                                         gtgATTGCAGTGCCAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAg               >  2:61214/1‑139 (MQ=255)                                                                                                                     ccAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAGGTAGATTGCCCg   >  1:685396/1‑139 (MQ=255)                                                                                                                     ccAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAGGTAGATTGCCCg   <  1:855867/139‑1 (MQ=255)                                                                                                                     ccAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAGGTAGATTGCCCg   >  1:1039792/1‑139 (MQ=255)                                                                                                                     ccAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAGGTAGATTGCCCg   >  1:950138/1‑139 (MQ=255)                                                                                                                      cAGGATCCGAACGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAGGTAGATTGCCCGt  <  1:622273/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                 TGGCTTATTTTCAATGAGATAATGCTGGAGGCGTGCTTTTTCGCCACGATAAGCCAGCGCGGTCGCGTAAGTAATATATGACTGAGGATCGGTGAAGATCCCCTGTGATTGCAGTGCCAGGATCCGATCGTCCAGCTGCCCGGCAAGAAGCACGTCAAACCACTGACGGCGTTCTGCCGCGCTTAATGTGTTCTGCTGGCGTGCTTCATTGTATAGCGTATCTGCCTGGGACCATTGTTTCAGGTAGATTGCCCGT  >  NZ_CP009273/585577‑585832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑