| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 599,729 | C→T | A131T (GCA→ACA) | ybdG ← | mechanosensitive ion channel YbdG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 599,729 | 0 | C | T | 89.3% | 176.1 / 9.1 | 56 | A131T (GCA→ACA) | ybdG | mechanosensitive ion channel YbdG |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (20/30); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
ACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGAATAC > NZ_CP009273/599601‑599864 | aCCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTc > 1:1089285/1‑139 (MQ=255) aaGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTgc > 2:303146/1‑139 (MQ=255) cTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGAt > 1:832401/1‑139 (MQ=255) cTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGAt > 1:266212/1‑139 (MQ=255) tCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAAc < 1:541543/139‑1 (MQ=255) cTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATc > 1:1195175/1‑139 (MQ=255) cTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATc > 1:397171/1‑139 (MQ=255) cGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGc < 1:507770/139‑1 (MQ=255) ccAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACgaga < 1:597235/139‑1 (MQ=255) ccAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACgaga < 2:397171/139‑1 (MQ=255) aaTCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGagaag < 1:275956/139‑1 (MQ=255) agcGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGa > 2:810623/1‑137 (MQ=255) gcGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAag < 2:941925/139‑1 (MQ=255) gcGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAag < 2:459649/139‑1 (MQ=255) gcGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAag < 2:249388/139‑1 (MQ=255) cGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAag > 1:408605/1‑138 (MQ=255) agaTCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAagcag < 1:339809/139‑1 (MQ=255) gaTCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAagcagc < 1:360092/139‑1 (MQ=255) atcaAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCa < 2:1009905/138‑1 (MQ=255) atcaAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCa < 2:408605/138‑1 (MQ=255) atcaAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCa < 2:266212/138‑1 (MQ=255) atcaAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCa < 2:294256/138‑1 (MQ=255) aTGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca < 1:665187/139‑1 (MQ=255) aTGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca < 1:703605/139‑1 (MQ=255) aTGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca < 1:980905/139‑1 (MQ=255) aTGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTGTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca < 1:547327/139‑1 (MQ=255) ccGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATg < 2:832401/138‑1 (MQ=255) gcgcCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTgcg < 2:31164/139‑1 (MQ=255) cgcCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTgcgc > 1:1000715/1‑139 (MQ=255) cgcCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTgcgc > 1:44285/1‑139 (MQ=255) gATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAg < 1:159151/139‑1 (MQ=255) aTCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAgg < 2:475881/139‑1 (MQ=255) tCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGaa > 2:20354/1‑138 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGcc < 1:1163325/51‑1 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGcc > 2:1163325/1‑51 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACgaga < 1:835702/79‑1 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACgaga > 2:835702/1‑79 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCa > 1:1004318/1‑96 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCa < 2:1004318/96‑1 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTg > 2:952610/1‑139 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTg > 1:214578/1‑139 (MQ=255) tGAAATATCCCTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTg > 2:443081/1‑139 (MQ=255) cccTTTCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCg > 2:811904/1‑139 (MQ=255) cccTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCaaaa < 1:60307/53‑1 (MQ=255) cccTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCaaaa > 2:60307/1‑53 (MQ=255) ttCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca > 1:684320/1‑91 (MQ=255) ttCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca < 2:684320/91‑1 (MQ=255) ttCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGc > 1:231116/1‑139 (MQ=255) tCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGcc < 2:159186/38‑1 (MQ=255) tCAGCGGTAACTGAGATGCTGTCGGGAATTTCTGCGcc > 1:159186/1‑38 (MQ=255) cGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCtttt > 2:669418/1‑139 (MQ=255) tAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGc < 2:708754/139‑1 (MQ=255) tAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGc < 1:811904/139‑1 (MQ=255) tAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGc < 1:230394/139‑1 (MQ=255) tAACTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGc < 2:1000715/139‑1 (MQ=255) cTGAGATGCTGTCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGc < 1:439419/139‑1 (MQ=255) tgctgtCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca > 2:669964/1‑74 (MQ=255) tgctgtCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATAcatca < 1:669964/74‑1 (MQ=255) ctgtCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGAATAc < 1:810623/139‑1 (MQ=255) ctgcCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGAATAc < 1:669418/139‑1 (MQ=255) | ACCAAGACCGCTGATCAGAATCGCTGGCGACTGACCAATCAGCAGCGAGATCATCAAAATGCCGACCAGAATCGCGCCGATCAGTTTAATCCCCTGAAATATCCCTTTCAGCGGTAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGCCAGAATAC > NZ_CP009273/599601‑599864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |