| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 624,200 | G→A | R65Q (CGA→CAA) | entA → | 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase EntA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 624,200 | 0 | G | A | 94.0% | 163.4 / 0.7 | 50 | R65Q (CGA→CAA) | entA | 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase EntA |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (22/25); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 | |||||||||||
GGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGCGTTTAACC > NZ_CP009273/624069‑624334 | ggCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATACCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGt > 1:412154/1‑137 (MQ=255) gcgcTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAg > 1:587985/1‑139 (MQ=255) cTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGGTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGGAGTGATGGATGTTGCCGACGCTGCGCAGGGCGCGCAAGTGTGTCAGCAACTGTTAGCt > 2:985172/1‑138 (MQ=255) gCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGaaa < 2:952564/139‑1 (MQ=255) gCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGaaa > 1:1189811/1‑139 (MQ=255) gCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTgg < 2:604559/139‑1 (MQ=255) aGCGAAAGTTACAGGTTTTGAGCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGAcgcg < 2:393674/139‑1 (MQ=255) aaGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGt < 2:61285/139‑1 (MQ=255) aaGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGt > 1:1100029/1‑139 (MQ=255) ttGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCggg > 2:1286154/1‑139 (MQ=255) ttGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGcggcgg < 1:663155/138‑1 (MQ=255) ttGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCggg > 2:797253/1‑139 (MQ=255) ttGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTATCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCggg > 2:972217/1‑139 (MQ=255) aGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATgcggc > 2:500805/1‑129 (MQ=255) aGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATgcggc < 1:500805/129‑1 (MQ=255) gTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGc < 1:1277622/139‑1 (MQ=255) ttCACTCAGGAGCAATACCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCa < 2:587985/139‑1 (MQ=255) cTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATggg < 1:643111/139‑1 (MQ=255) cTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATggg < 2:172292/139‑1 (MQ=255) aGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGGATTTTACGCAAgggg > 2:414318/1‑136 (MQ=255) aGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGc < 2:980156/131‑1 (MQ=255) aGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGc > 1:980156/1‑131 (MQ=255) aGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGgcgc > 1:1016947/1‑139 (MQ=255) gAGCAATAGCCCTTTGCGACCGAAGTGATGGATGTTGCCGGCGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGa < 2:389411/139‑1 (MQ=255) atatCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGAt < 1:908210/139‑1 (MQ=255) atatCCCTGTGCGACCTAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCTCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGAt < 2:941414/139‑1 (MQ=255) tatCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAAtt > 2:118473/1‑117 (MQ=255) tatCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAAtt < 1:118473/117‑1 (MQ=255) tatCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGAt > 1:160640/1‑138 (MQ=255) tCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAg < 1:466106/139‑1 (MQ=255) ccTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCt < 2:727624/139‑1 (MQ=255) ccTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCt < 1:423671/139‑1 (MQ=255) ccTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCt < 1:414318/139‑1 (MQ=255) tGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCa < 2:2868/132‑1 (MQ=255) tGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCa > 1:2868/1‑132 (MQ=255) tGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAg > 2:291431/1‑133 (MQ=255) tGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAg < 1:291431/133‑1 (MQ=255) tGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACt > 1:382847/1‑139 (MQ=255) tGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACt > 2:1269783/1‑139 (MQ=255) ccGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGGAAAGAGGACTGGCAGCAGACttt > 2:960359/1‑139 (MQ=255) cgcAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTcggcgg < 2:376546/139‑1 (MQ=255) cgcAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTcggcgg < 2:272484/139‑1 (MQ=255) gcAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCCCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGGTAACGTcggcggg > 2:34359/1‑138 (MQ=255) gcAAGTGTGTCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGt > 2:213109/1‑139 (MQ=255) tgtgtCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTTGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGAt < 2:1261194/83‑1 (MQ=255) tgtgtCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGAt > 1:1261194/1‑83 (MQ=255) tgtCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCCGTAAAGAGGGCTGGCAGCCGGCTTTTGGGGGTAAAGTCGGCGGGGCGgtt > 2:694219/1‑138 (MQ=255) tCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGCGTTTAAc < 1:681975/139‑1 (MQ=255) tCAGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGCGTTTAAc > 1:556343/1‑139 (MQ=255) aGCAACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGCGTTTAAcc < 2:556343/138‑1 (MQ=255) | GGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCGCAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCGGGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTTGCGGTTAACGTCGGCGGTGCGTTTAACC > NZ_CP009273/624069‑624334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |