Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	628,572	G→A	A121V (GCC→GTC)	hcxA ←	hydroxycarboxylate dehydrogenase HcxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	628,572	0	G	A	100.0%	203.2 / NA	56	A121V (GCC→GTC)	hcxA	hydroxycarboxylate dehydrogenase HcxA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

TTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGT  >  NZ_CP009273/628442‑628703                                                                                                                                   |                                                                                                                                    ttgCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGAtc                                                                                                                             >  2:489074/1‑139 (MQ=255)     ggTGCATTTAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGAtcgtcg                                                                                                                         >  2:271876/1‑139 (MQ=255)         cATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAAc                                                                                                                     >  2:523725/1‑139 (MQ=255)           ttGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACgg                                                                                                                   >  2:310390/1‑139 (MQ=255)                 aTAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGcaaca                                                                                                              >  1:84768/1‑138 (MQ=255)                   aaTCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAAc                                                                                                           >  2:91888/1‑139 (MQ=255)                   aaTCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAAc                                                                                                           >  2:300059/1‑139 (MQ=255)                         cGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACgg                                                                                                         <  2:784679/135‑1 (MQ=255)                          ggTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGAc                                                                                                    >  2:384901/1‑139 (MQ=255)                                  caGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGAcgg                                                                                            <  1:300059/139‑1 (MQ=255)                                          tAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGAt                                                                                                                              <  1:1179315/97‑1 (MQ=255)                                          tAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGAt                                                                                                                              >  2:1179315/1‑97 (MQ=255)                                          tAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAg                                                                                    >  1:200411/1‑139 (MQ=255)                                          tAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAg                                                                                    >  1:881808/1‑139 (MQ=255)                                                  gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcg                                                                            <  1:384901/139‑1 (MQ=255)                                                  gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcg                                                                            <  1:523725/139‑1 (MQ=255)                                                  gCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcg                                                                            <  1:579997/139‑1 (MQ=255)                                                    gtcgtcGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                          <  2:84768/139‑1 (MQ=255)                                                    gtcgtcGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                          <  1:342804/139‑1 (MQ=255)                                                    gtcgtcGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                          <  1:434908/139‑1 (MQ=255)                                                       gtcgAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGc                                                                                 >  2:625444/1‑129 (MQ=255)                                                       gtcgAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGc                                                                                 <  1:625444/129‑1 (MQ=255)                                                          gAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTc                                                                     <  1:1072307/138‑1 (MQ=255)                                                                   aTAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTg                                                             <  1:771959/137‑1 (MQ=255)                                                                               cTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACg                                               <  1:91888/139‑1 (MQ=255)                                                                               cTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACg                                               <  1:281702/139‑1 (MQ=255)                                                                               cTGTCCGGCATCATTATACCAGACGGAGAGCGGTGGCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACg                                               <  2:292224/139‑1 (MQ=255)                                                                                 gTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                          >  2:670521/1‑110 (MQ=255)                                                                                 gTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                          <  1:670521/110‑1 (MQ=255)                                                                                  tCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGcc                                             <  1:310390/138‑1 (MQ=255)                                                                                         tcatTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATcacca                                     >  2:156410/1‑139 (MQ=255)                                                                                               taCCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTg                                <  1:484272/138‑1 (MQ=255)                                                                                                    gACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTg                                >  1:253351/1‑133 (MQ=255)                                                                                                    gACGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTg                                <  2:253351/133‑1 (MQ=255)                                                                                                    gACGGAGAGCGGTGTCCAGGAGGCGCAGGTGACGGCCATCGTCGGAACGGCAACAAACGGCAGCCCGAGACGGCGGGCGAGGGACTTCGCGGTGTCGAGCAGTGCACCGCCACCGCCGCCAATCACCAAGCTGCGggcg                          >  2:98234/1‑139 (MQ=255)                                                                                                     aCGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAg                                                                  <  2:950281/98‑1 (MQ=255)                                                                                                     aCGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAg                                                                  >  1:950281/1‑98 (MQ=255)                                                                                                     aCGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGgtcgt                         >  2:28471/1‑139 (MQ=255)                                                                                                     aCGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGgt                            >  2:260149/1‑136 (MQ=255)                                                                                                     aCGGAGAGCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGgt                            <  1:260149/136‑1 (MQ=255)                                                                                                         agagCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTg                                                             >  2:809958/1‑99 (MQ=255)                                                                                                         agagCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTg                                                             <  1:809958/99‑1 (MQ=255)                                                                                                         agagCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCAcc                     >  1:173026/1‑139 (MQ=255)                                                                                                           agCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGCGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCg                    >  1:366128/1‑138 (MQ=255)                                                                                                           agCGGTGTCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGGGTCGAGCAGGGCACCGCCACCGACGCCAATCACCACGCTGCGGGCGTCACCg                    >  2:1022535/1‑138 (MQ=255)                                                                                                               gtgtCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACt                >  1:1242893/1‑138 (MQ=255)                                                                                                                tgtCCAGGCGGCGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACt                >  1:276481/1‑137 (MQ=255)                                                                                                                      ggcggcGCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAgccgcc        <  2:200411/139‑1 (MQ=255)                                                                                                                       gcggcgCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAgccgc         >  2:1278161/1‑137 (MQ=255)                                                                                                                        cggcgCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAg      <  1:877659/139‑1 (MQ=255)                                                                                                                        cggcgCAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAg      <  1:780327/139‑1 (MQ=255)                                                                                                                          gcgcAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                          >  2:871835/1‑69 (MQ=255)                                                                                                                          gcgcAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTcgcg                                                                          <  1:871835/69‑1 (MQ=255)                                                                                                                          gcgcAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTc                                                                     <  2:690034/74‑1 (MQ=255)                                                                                                                          gcgcAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTc                                                                     >  1:690034/1‑74 (MQ=255)                                                                                                                            gcAGGTGACGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAgttgt  >  2:455521/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                    TTGCGGTGCATTGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGT  >  NZ_CP009273/628442‑628703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑