| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 633,831 | C→T | A67T (GCT→ACT) | dsbG ← | thiol:disulfide interchange protein DsbG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 633,831 | 0 | C | T | 86.5% | 97.9 / 7.4 | 37 | A67T (GCT→ACT) | dsbG | thiol:disulfide interchange protein DsbG |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (11/21); total (13/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
ACCGTCGAGGAGCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACT > NZ_CP009273/633700‑633961 | aCCGTCGAGGAGCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCtt < 2:80815/138‑1 (MQ=255) gaggagCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATc < 2:1219732/138‑1 (MQ=255) gCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGt < 1:296968/139‑1 (MQ=255) ccAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTc < 2:723901/139‑1 (MQ=255) gTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGt > 2:706258/1‑139 (MQ=255) ccATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGa < 1:1217880/138‑1 (MQ=255) tCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGcc < 2:178231/139‑1 (MQ=255) cGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCa < 1:19304/139‑1 (MQ=255) cGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCa < 2:693167/139‑1 (MQ=255) cGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCa < 1:319227/139‑1 (MQ=255) cGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCa < 1:706258/139‑1 (MQ=255) tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCtt < 1:409320/104‑1 (MQ=255) tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCtt > 1:970779/1‑104 (MQ=255) tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCtt < 2:970779/104‑1 (MQ=255) tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCtt > 2:409320/1‑104 (MQ=255) tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCatat > 2:665104/1‑139 (MQ=255) tGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCatat > 2:56650/1‑139 (MQ=255) caTTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGa < 2:679234/139‑1 (MQ=255) gCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGAt < 1:1056866/139‑1 (MQ=255) cTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATa < 2:1260072/139‑1 (MQ=255) tAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCa > 2:964568/1‑139 (MQ=255) tAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCa < 2:977817/139‑1 (MQ=255) tAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCa < 1:209284/139‑1 (MQ=255) tttCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCgg < 1:56650/138‑1 (MQ=255) aGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGa < 2:391190/139‑1 (MQ=255) aCTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGtttt < 2:49558/138‑1 (MQ=255) cTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTtga > 2:666791/1‑139 (MQ=255) aGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTtgat < 2:1110907/137‑1 (MQ=255) aGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGt > 2:428745/1‑59 (MQ=255) aGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGt < 1:428745/59‑1 (MQ=255) gTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGa > 2:1160599/1‑71 (MQ=255) gTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGa < 1:1160599/71‑1 (MQ=255) aaCCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCttt > 2:885377/1‑139 (MQ=255) aCCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCttt > 2:830586/1‑138 (MQ=255) aCCAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCttt > 1:302514/1‑138 (MQ=255) cAGAGATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTAc > 2:813494/1‑139 (MQ=255) agagATAGTGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACt < 1:666791/139‑1 (MQ=255) | ACCGTCGAGGAGCCAGTGGGATTGTTCCATCCGTTGCCACATTTCGCGTCCGGCTGGTGCGTAAATTTCTTTTTCGATAAGTGTGTTACTCAGGTTTTCACCTTTCTCGTTGTACATGTAACCAGAGATAGCGTGCTTACCATCTGGAGTCAGGTAGATGGTGACGCCCATATCCTGATACTTTCCGAGATAACCTTTCATTCCTCCGGGGGCATCGAATGTTTTGATGATTGTAATGCCCTGTTTTTCAATCGCTTTTACT > NZ_CP009273/633700‑633961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |