| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 635,912 | G→A | G235D (GGC→GAC) | ahpF → | alkyl hydroperoxide reductase subunit F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 635,912 | 0 | G | A | 91.1% | 167.5 / 6.7 | 56 | G235D (GGC→GAC) | ahpF | alkyl hydroperoxide reductase subunit F |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base A (23/28); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01 | |||||||||||
AAATCGTTGCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGGCATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATA > NZ_CP009273/635777‑636047 | aaaTCGTTGCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACAt < 1:888723/139‑1 (MQ=255)aaaTCGTTGCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACAt < 2:790792/139‑1 (MQ=255)aaaTCGTTGCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACAt < 2:748659/139‑1 (MQ=255) gTTGCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTa < 2:238971/139‑1 (MQ=255) ttGCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTAc > 2:456384/1‑139 (MQ=255) gCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTAcc < 2:73677/138‑1 (MQ=255) aCTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTAATCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCg > 2:342803/1‑139 (MQ=255) gcgcGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACg > 1:299632/1‑139 (MQ=255) aaaaCGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTtggt < 2:299632/139‑1 (MQ=255) cGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGGCATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTc < 1:879022/139‑1 (MQ=255) tGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAg < 2:443669/139‑1 (MQ=255) tGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAg < 1:986221/139‑1 (MQ=255) ggCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGGGCAGCGGCAGCCATTTAATCCGCACGTAAAGACATCCGTACCGGTGTGATGGGCGAAAGTTTTGGGGGGCAGATc > 2:915895/1‑139 (MQ=255) ggCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGGGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGGGGTCAGATc > 2:915840/1‑139 (MQ=255) cAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCt < 2:1093621/139‑1 (MQ=255) gaagaGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCg < 2:155704/139‑1 (MQ=255) tGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGt > 1:986970/1‑139 (MQ=255) gTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCg > 2:1135331/1‑138 (MQ=255) tGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGaa < 2:986970/139‑1 (MQ=255) tGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGaa < 1:700328/139‑1 (MQ=255) aTGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCCGCAATTTACTCCGCACGTAAAGGCATCCGTACCGGTCTGATGGGCGAACGTTTTGGGGGTCAGATCCTCGATACCGTTGATATCGaaaa > 1:211439/1‑139 (MQ=255) aTGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGaaaa > 2:294054/1‑139 (MQ=255) aTGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGaaaa > 1:297765/1‑139 (MQ=255) aTGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGGGCAGCAGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGaaa > 2:1198529/1‑138 (MQ=255) cTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTa < 1:342803/139‑1 (MQ=255) aTGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACAt > 1:568506/1‑139 (MQ=255) tGACGTATTAATCGTCGGTTCCGGCCCGGCGGGGGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACAtt > 2:1062409/1‑139 (MQ=255) cGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCt < 2:568506/139‑1 (MQ=255) aTTAATCGTCGGTTCCGGCCCGGCGGGTGCCGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATTGAAAACTACATTTCTGTa > 1:484079/1‑139 (MQ=255) aTTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGGCATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCg > 1:689465/1‑107 (MQ=255) aTTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGGCATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCg < 2:689465/107‑1 (MQ=255) aTTAATCGTCGGTTCCGGCCCGGCGGGGGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTa > 1:1230871/1‑139 (MQ=255) aTTAATCGTCGGTTCCGGCCCGGCGGGGGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGGTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTa > 2:918448/1‑139 (MQ=255) ttAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTAc > 2:342239/1‑139 (MQ=255) aaTCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTAcc < 1:862130/138‑1 (MQ=255) aaTCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTAcc < 1:456384/138‑1 (MQ=255) tcgtcgGTTGCGGCACGGCGGGTGCAGCCGCAGCAAGTGACTCTGCACGTAAAGACATCCGCACCGGTCTGATGGACGAACGTTTTGGTGGTCAGATCCTCGAGACCGTTGATATCGAAAACTTCATTTCTGTACCGaa < 2:1219159/139‑1 (MQ=255) tcgtcgGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGaa < 2:297765/139‑1 (MQ=255) cgGTTCCGGTCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACt > 1:882448/1‑139 (MQ=255) cgGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACt > 1:1070960/1‑139 (MQ=255) gTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGa < 1:342239/139‑1 (MQ=255) gTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGa < 1:1135331/139‑1 (MQ=255) ccggcGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGa < 1:116698/139‑1 (MQ=255) gcggGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGc > 1:1290965/1‑139 (MQ=255) cAGCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAgg < 1:294054/139‑1 (MQ=255) gCGGCAGCAATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGgcg < 2:665518/139‑1 (MQ=255) cGGCAGCAATTTACTCCGCACGTAAAGGCATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGgcg > 2:1097537/1‑138 (MQ=255) gcaATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGaaaa > 1:1135639/1‑89 (MQ=255) gcaATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGaaaa < 2:1135639/89‑1 (MQ=255) gcaATTTACTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTg > 2:31990/1‑138 (MQ=255) cTCCGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCa < 1:1062409/138‑1 (MQ=255) cGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACg < 2:484079/137‑1 (MQ=255) cGCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGtt > 2:205593/1‑139 (MQ=255) gCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGtt > 2:14093/1‑138 (MQ=255) gCACGTAAAGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTtg < 2:291950/139‑1 (MQ=255) aaGACATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATa < 2:105295/139‑1 (MQ=255) | AAATCGTTGCCAAAATTGATACTGGCGCGGAAAAACGTGCGGCAGAAGAGCTGAACAAGCGTGATGCTTATGACGTATTAATCGTCGGTTCCGGCCCGGCGGGTGCAGCGGCAGCAATTTACTCCGCACGTAAAGGCATCCGTACCGGTCTGATGGGCGAACGTTTTGGTGGTCAGATCCTCGATACCGTTGATATCGAAAACTACATTTCTGTACCGAAGACTGAAGGGCAGAAGCTGGCAGGCGCACTGAAAGTTCACGTTGATGAATA > NZ_CP009273/635777‑636047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |