Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 683,163 G→A A14V (GCG→GTG)  gltI ← glutamate/aspartate ABC transporter substrate‑binding protein GltI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273683,1630GA94.3% 241.2 / 3.2 70A14V (GCG→GTG) gltIglutamate/aspartate ABC transporter substrate‑binding protein GltI
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (34/32);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

ATTGTCGTAATAAGAGAAAGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTG  >  NZ_CP009273/683030‑683298
                                                                                                                                     |                                                                                                                                       
aTTGTCGTAATAAGAGAAAGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAgg                                                                                                                                    >  2:235747/1‑139 (MQ=255)
              agaAAGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGc                                                                                                                                  <  2:1183869/127‑1 (MQ=255)
              agaAAGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGc                                                                                                                                  >  1:1183869/1‑127 (MQ=255)
              agaAAGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCtgt                                                                                                                      >  1:1290492/1‑139 (MQ=255)
                aaaGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTgg                                                                                                                    >  2:289703/1‑139 (MQ=255)
                aaaGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTgg                                                                                                                    >  1:514513/1‑139 (MQ=255)
                 aaGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGc                                                                                                                   <  2:198073/139‑1 (MQ=255)
                 aaGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGc                                                                                                                   <  2:833510/139‑1 (MQ=255)
                  aGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGCAAGCACCAGGGCGAGGATTGCTGTgg                                                                                                                    >  1:8618/1‑137 (MQ=255)
                  aGGCACTGAAGATTCACGGAGACCAACGACAATCACACCGTTTTTGGCGATTTTATCCAGAGTACTACCCGCTGCCGGGGCGGCGTCGTCTGCCTGGGCCAGTCCTGAGGAAAGCACCAGGGCGAGGATTGCtgggt                                                                                                                    >  2:1287057/1‑134 (MQ=255)
                   ggCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAg                                                                                                                 >  1:409024/1‑139 (MQ=255)
                               tCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGGAAtt                                                                                                     >  1:833216/1‑139 (MQ=255)
                                      gACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATc                                                                                              >  2:1015267/1‑139 (MQ=255)
                                        ccGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCa                                                                                            <  2:514513/139‑1 (MQ=255)
                                         cgacgaCAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCaa                                                                                           <  1:271405/139‑1 (MQ=255)
                                         cgacgaCAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCaa                                                                                           <  1:289703/139‑1 (MQ=255)
                                         cgacgaCAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCaa                                                                                           <  2:698076/139‑1 (MQ=255)
                                                 aTCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTa                                                                                   >  2:306552/1‑139 (MQ=255)
                                                     cacCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCTGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTAtctt                                                                               <  1:1256441/139‑1 (MQ=255)
                                                          tttttGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCg                                                                          >  1:1215459/1‑139 (MQ=255)
                                                          tttttGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCg                                                                          >  1:60886/1‑139 (MQ=255)
                                                          tttttGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGGTTACGGAATTGCATATCCAACTCCTTTATCTTCTGCg                                                                          >  2:715190/1‑139 (MQ=255)
                                                                gCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAg                                                                     <  1:319834/138‑1 (MQ=255)
                                                                 cGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGaa                                                                   <  2:1215459/139‑1 (MQ=255)
                                                                      ttGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCACCTCCTTTATCTTCGGCGTTAAAAACGCa                                                               <  2:88881/138‑1 (MQ=255)
                                                                        gTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATg                                                             <  1:500652/138‑1 (MQ=255)
                                                                        gTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATg                                                             <  2:586427/138‑1 (MQ=255)
                                                                             gagTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATAcc                                                       <  1:1147736/139‑1 (MQ=255)
                                                                                   tGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCttt                                                                                    <  1:771241/104‑1 (MQ=255)
                                                                                   tGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCttt                                                                                    >  2:771241/1‑104 (MQ=255)
                                                                                      ccGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAg                                              <  1:1005824/139‑1 (MQ=255)
                                                                                        gCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAg                                                                     <  2:794261/114‑1 (MQ=255)
                                                                                        gCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAg                                                                     >  1:794261/1‑114 (MQ=255)
                                                                                        gCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAgtg                                            >  2:1164338/1‑139 (MQ=255)
                                                                                        gCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAgtg                                            >  1:1073184/1‑139 (MQ=255)
                                                                                          tGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTTTGCGTGtt                                          >  2:412326/1‑139 (MQ=255)
                                                                                          tGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGtt                                          >  1:918325/1‑139 (MQ=255)
                                                                                            ccGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTAtcttc                                                                              >  2:292744/1‑101 (MQ=255)
                                                                                            ccGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTAtcttc                                                                              <  1:292744/101‑1 (MQ=255)
                                                                                                    gcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATc                                                                                              >  1:16328/1‑77 (MQ=255)
                                                                                                    gcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATc                                                                                              <  2:16328/77‑1 (MQ=255)
                                                                                                    gcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTa                                >  1:684133/1‑139 (MQ=255)
                                                                                                    gcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTa                                >  2:556004/1‑139 (MQ=255)
                                                                                                       tCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTtgt                                                 <  2:993355/119‑1 (MQ=255)
                                                                                                       tCATCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTtgt                                                 >  1:993355/1‑119 (MQ=255)
                                                                                                          tCTGCCTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCt                          >  1:1162595/1‑139 (MQ=255)
                                                                                                              ccTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTg                         <  2:96353/136‑1 (MQ=255)
                                                                                                              ccTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTg                         <  2:352544/136‑1 (MQ=255)
                                                                                                              ccTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCaa                      <  2:1290492/139‑1 (MQ=255)
                                                                                                              ccTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCaa                      <  1:306552/139‑1 (MQ=255)
                                                                                                              ccTGTGCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCaa                      <  2:1073184/139‑1 (MQ=255)
                                                                                                                 gtgCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTAtcttct                                                                             <  1:858538/81‑1 (MQ=255)
                                                                                                                 gtgCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTAtcttct                                                                             >  2:858538/1‑81 (MQ=255)
                                                                                                                  tgCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGa                                                           >  1:649609/1‑98 (MQ=255)
                                                                                                                  tgCCAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGa                                                           <  2:649609/98‑1 (MQ=255)
                                                                                                                    ccAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTa                 <  2:868006/138‑1 (MQ=255)
                                                                                                                    ccAGTCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTa                 <  1:4959/138‑1 (MQ=255)
                                                                                                                        tCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGa                                                           <  2:1176457/92‑1 (MQ=255)
                                                                                                                        tCCTGCGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGa                                                           >  1:1176457/1‑92 (MQ=255)
                                                                                                                           tGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCg                                                     >  1:805215/1‑95 (MQ=255)
                                                                                                                           tGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCg                                                     <  2:805215/95‑1 (MQ=255)
                                                                                                                             cGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATtgtg            <  1:425507/134‑1 (MQ=255)
                                                                                                                             cGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtg       >  1:1081947/1‑139 (MQ=255)
                                                                                                                             cGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtg       >  1:355138/1‑139 (MQ=255)
                                                                                                                             cGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtg       >  1:623074/1‑139 (MQ=255)
                                                                                                                             cGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgtg       >  2:1215566/1‑139 (MQ=255)
                                                                                                                             cGGAAAGCACCAGGGCGAGGATTGCTGTGGCAGGGTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATtgtg            >  2:425507/1‑134 (MQ=255)
                                                                                                                                 aaGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGtgttgt   <  2:684133/139‑1 (MQ=255)
                                                                                                                                  aGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTtgtg  >  1:240092/1‑139 (MQ=255)
                                                                                                                                  aGCACCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTtgtg  <  1:556004/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
ATTGTCGTAATAAGAGAAAGGCACTGAAGATTCACGGTGACCGACGACAATCACACCGTTTTTGGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTG  >  NZ_CP009273/683030‑683298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑