| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 714,441 | A→T | D492E (GAT→GAA) | speF ← | ornithine decarboxylase SpeF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 714,441 | 0 | A | T | 96.8% | 246.7 / ‑0.4 | 63 | D492E (GAT→GAA) | speF | ornithine decarboxylase SpeF |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (28/33); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
TTAAGATCGCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAG > NZ_CP009273/714307‑714572 | ttAAGATCGCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGa < 2:734429/139‑1 (MQ=255) aGATCGCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACaa < 2:1181934/139‑1 (MQ=255) aGATCGCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACa > 1:721488/1‑138 (MQ=255) gCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACa < 2:1248553/133‑1 (MQ=255) gCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACa > 1:1248553/1‑133 (MQ=255) gCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTAt > 1:1063150/1‑139 (MQ=255) gCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTAt > 1:1158762/1‑139 (MQ=255) cATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTAtt > 2:1255027/1‑139 (MQ=255) aTTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTg < 1:1264216/139‑1 (MQ=255) tttCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGAt < 2:134283/138‑1 (MQ=255) ttCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGAtt < 1:1279178/138‑1 (MQ=255) cTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATa < 2:190168/139‑1 (MQ=255) cTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATa < 1:645246/139‑1 (MQ=255) cGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGcc > 2:896718/1‑139 (MQ=255) ttttCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCCTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCa > 2:477409/1‑138 (MQ=255) tCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCaaaa < 2:69385/138‑1 (MQ=255) cgcAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAAt > 2:461331/1‑139 (MQ=255) cAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGc < 1:1259578/139‑1 (MQ=255) aGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGcc < 2:743576/139‑1 (MQ=255) tAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTc > 2:114862/1‑139 (MQ=255) aTCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTa < 1:748198/139‑1 (MQ=255) tcgtcgCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTAc < 2:721488/139‑1 (MQ=255) tcgCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACaa < 1:571637/84‑1 (MQ=255) tcgCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACaa > 2:571637/1‑84 (MQ=255) aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGACTGCCAGTGTTCCCCTGGTACAACCTGGAAGAAgcg > 2:228573/1‑137 (MQ=255) aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAgcg > 1:73766/1‑137 (MQ=255) aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAgcg > 2:874846/1‑137 (MQ=255) aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAgcg > 1:571389/1‑137 (MQ=255) aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAgcg > 1:419835/1‑137 (MQ=255) ccGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAAttt > 2:1255415/1‑139 (MQ=255) ttACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgcg < 1:461331/139‑1 (MQ=255) ttACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgcg < 2:73766/139‑1 (MQ=255) ttACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTAGAAGAAGCGCAGATCAACCGCAATTTgcg < 2:296579/139‑1 (MQ=255) aCGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgcgc < 2:1264409/138‑1 (MQ=255) aTCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCa > 1:656343/1‑139 (MQ=255) aTACCTGGGGTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACg < 1:1189534/139‑1 (MQ=255) ggggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGc > 1:973239/1‑138 (MQ=255) gggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATa < 2:149616/52‑1 (MQ=255) gggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATa > 1:149616/1‑52 (MQ=255) gggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGcc < 1:305244/138‑1 (MQ=255) gggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGcc < 2:695656/138‑1 (MQ=255) gggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCa < 1:896718/139‑1 (MQ=255) gggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCa < 1:774105/139‑1 (MQ=255) gggTTGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCa < 2:419835/139‑1 (MQ=255) ttGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAggg > 2:193654/1‑139 (MQ=255) tGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACt < 2:1115017/86‑1 (MQ=255) tGTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACt > 1:1115017/1‑86 (MQ=255) gTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgcg < 2:1052107/115‑1 (MQ=255) gTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgcg > 1:1052107/1‑115 (MQ=255) gTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATAc < 1:1235952/126‑1 (MQ=255) gTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATAc > 2:1235952/1‑126 (MQ=255) gTCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGtt > 1:62943/1‑139 (MQ=255) tCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTcccc < 2:562277/73‑1 (MQ=255) tCAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTcccc > 1:562277/1‑73 (MQ=255) cAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAgtgt < 1:319118/67‑1 (MQ=255) cAGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAgtgt > 2:319118/1‑67 (MQ=255) aGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTa < 1:114862/138‑1 (MQ=255) aGCAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTAc < 1:1255415/139‑1 (MQ=255) cAACAGTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCa < 2:216244/139‑1 (MQ=255) gTTTGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATccac < 2:973173/139‑1 (MQ=255) tttGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATccac > 1:367391/1‑138 (MQ=255) tttGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATccac > 1:869077/1‑138 (MQ=255) tGCATGGTTCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAg > 1:679007/1‑139 (MQ=255) | TTAAGATCGCATTTTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAG > NZ_CP009273/714307‑714572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |