Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 721,666 A→G V276A (GTC→GCC)  kdpB ← potassium‑transporting ATPase subunit KdpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273721,6660AG96.4% 183.8 / ‑0.9 56V276A (GTC→GCC) kdpBpotassium‑transporting ATPase subunit KdpB
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (23/31);  total (23/33)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCG  >  NZ_CP009273/721533‑721780
                                                                                                                                     |                                                                                                                  
cGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGcc                                                                                                                >  1:748204/1‑138 (MQ=255)
      cTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCt                                                                                                         <  1:96412/139‑1 (MQ=255)
       tGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCt                                                                                                         >  1:663733/1‑138 (MQ=255)
               aCCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAgg                                                                                                >  1:584988/1‑139 (MQ=255)
                     tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGTCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAg                                                                                                 <  2:436904/132‑1 (MQ=255)
                     tgtgATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGTCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAg                                                                                                 >  1:436904/1‑132 (MQ=255)
                         aTGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATa                                                                                       <  1:548274/138‑1 (MQ=255)
                         aTGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAg                                                                                      <  1:132278/139‑1 (MQ=255)
                             tGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                  <  1:724898/139‑1 (MQ=255)
                             tGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                  >  1:710094/1‑139 (MQ=255)
                             tGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                  >  2:727200/1‑139 (MQ=255)
                             tGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                  >  2:254477/1‑139 (MQ=255)
                             tGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                  <  1:1156321/139‑1 (MQ=255)
                               ccGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAg                                                                                                 >  1:382860/1‑122 (MQ=255)
                               ccGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAg                                                                                                 <  2:382860/122‑1 (MQ=255)
                               ccGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGgcc                                                                                              <  2:1280031/125‑1 (MQ=255)
                               ccGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGgcc                                                                                              >  1:1280031/1‑125 (MQ=255)
                               ccGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                  >  1:72958/1‑137 (MQ=255)
                               ccGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGt                                                                                  <  2:72958/137‑1 (MQ=255)
                               ccGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTg                                                                                >  2:1134192/1‑139 (MQ=255)
                               ccGGATCTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATAACATTCCCGCCTATCCTCCGCCTCATCCCGGCCGCACCGATCGCAGCCAACCGTCCGCCATGAGTGGGTg                                                                                >  2:993106/1‑139 (MQ=255)
                                 ggTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTggg                                                                              <  2:1057503/139‑1 (MQ=255)
                                 ggTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTgg                                                                               >  1:689204/1‑138 (MQ=255)
                                        tCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                                                        <  1:727200/138‑1 (MQ=255)
                                        tCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                                                        <  1:955143/138‑1 (MQ=255)
                                        tCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                                                        <  2:584988/138‑1 (MQ=255)
                                             tAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAg                                                                                                 >  1:1247631/1‑108 (MQ=255)
                                             tAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAg                                                                                                 <  2:1247631/108‑1 (MQ=255)
                                               gCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACca                                                                 <  1:290267/138‑1 (MQ=255)
                                                  gAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                                                        >  2:359423/1‑128 (MQ=255)
                                                  gAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATcaga                                                                        <  1:359423/128‑1 (MQ=255)
                                                   aacgtcaacgtcACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCagcagc                                                            <  2:873953/139‑1 (MQ=255)
                                                   aacgtcaacgtcACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCagcagc                                                            <  2:352717/139‑1 (MQ=255)
                                                           cgtcaCCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAg                                                    <  2:583777/139‑1 (MQ=255)
                                                                       cTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt                                        <  2:710094/139‑1 (MQ=255)
                                                                       cTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt                                        <  2:748204/139‑1 (MQ=255)
                                                                       cTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt                                        <  2:948869/139‑1 (MQ=255)
                                                                       cTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt                                        <  1:1044488/139‑1 (MQ=255)
                                                                       cTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt                                        <  1:861301/139‑1 (MQ=255)
                                                                          cAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGAc                                     >  1:693677/1‑139 (MQ=255)
                                                                            aCTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTg                                   <  1:758522/139‑1 (MQ=255)
                                                                                cACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCAt                                >  2:313226/1‑138 (MQ=255)
                                                                                     ccGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGc                          >  1:1275407/1‑139 (MQ=255)
                                                                                       gCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGccc                        <  1:79016/139‑1 (MQ=255)
                                                                                        cTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGccc                        >  2:77633/1‑138 (MQ=255)
                                                                                           gtggCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCAcg                    <  2:1238209/139‑1 (MQ=255)
                                                                                                 aTCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGaa               >  2:885196/1‑138 (MQ=255)
                                                                                                      aTTCGCGCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGc         <  1:1162294/139‑1 (MQ=255)
                                                                                                         cgcgCCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCca       <  1:313226/138‑1 (MQ=255)
                                                                                                           cgcCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCca       >  2:590981/1‑136 (MQ=255)
                                                                                                           cgcCTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCca       <  1:590981/136‑1 (MQ=255)
                                                                                                             ccTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCg  <  2:1275407/139‑1 (MQ=255)
                                                                                                              cTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCca       >  1:885027/1‑133 (MQ=255)
                                                                                                              cTAGCATCCGGCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCca       <  2:885027/133‑1 (MQ=255)
                                                                                                                        gCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt                                        >  1:61075/1‑90 (MQ=255)
                                                                                                                        gCTCATCCCGGCGGCGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCt                                        <  2:61075/90‑1 (MQ=255)
                                                                                                                                     |                                                                                                                  
CGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCTCATCCCGGCGACGCCGATCGCTGACAACAGGCCGCCAATAGTGGTTGGGATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCG  >  NZ_CP009273/721533‑721780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑