| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 753,637 | A→G | N165D (AAT→GAT) | sdhB → | succinate dehydrogenase iron‑sulfur subunit SdhB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 753,637 | 0 | A | G | 89.4% | 125.3 / 7.0 | 47 | N165D (AAT→GAT) | sdhB | succinate dehydrogenase iron‑sulfur subunit SdhB |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (28/14); total (30/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
TTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGC > NZ_CP009273/753508‑753768 | ttGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 1:761526/1‑138 (MQ=255) tGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGAt > 1:621656/1‑137 (MQ=255) aaTCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCgg < 1:1211501/139‑1 (MQ=255) tCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTATTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCa < 1:537057/139‑1 (MQ=255) gATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTc < 2:621656/139‑1 (MQ=255) aTGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTcc > 1:1261164/1‑139 (MQ=255) gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTTCt > 2:1047927/1‑138 (MQ=255) agcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg < 2:1088907/139‑1 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCatat > 2:513582/1‑120 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCatat < 1:513582/120‑1 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGGTTGATAGcc > 2:531771/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTTGTAGcc > 2:26368/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTTATAGcc > 1:1165899/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:94488/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:1134121/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:758724/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:685970/1‑139 (MQ=255) cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc > 1:109147/1‑139 (MQ=255) cgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGt < 1:902815/139‑1 (MQ=255) gTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACaac > 2:725216/1‑137 (MQ=255) tGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCg < 1:1005604/139‑1 (MQ=255) tGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTTGCGGGATATTGTTTCCTGATTGATAGCCGTGATACCGGGGCTGACAGCCCCCCCg > 2:469471/1‑139 (MQ=255) tATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGGTTGTTTGCGGCCTTTTGTTTTCTGGTTTATAGCCGTGGTACCGGGACTGACAGCCGCCTCg > 2:1141059/1‑137 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc > 2:1235895/1‑137 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGAc < 1:1235895/137‑1 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACgg > 2:123382/1‑139 (MQ=255) ttCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACgg > 1:286709/1‑139 (MQ=255) ctctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGt < 2:109147/138‑1 (MQ=255) tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGttt > 2:420135/1‑137 (MQ=255) tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGttt > 1:944538/1‑137 (MQ=255) gcgcATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAg > 1:790291/1‑139 (MQ=255) aTGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGGGACTGACAGCCGCCTCGACGGGTTGAGTGAt > 1:512865/1‑139 (MQ=255) gttCAACCTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCAt < 2:790291/138‑1 (MQ=255) cAACCTCTTGTCCGTCTTTCTGGTGGAAGCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCa < 1:95257/138‑1 (MQ=255) ccTCTTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGGAGGGCTGTTCGCGGGCTCTCGGTTTCTTTTTGAGAGGCGCGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGAGGCATTTAGCg > 2:547588/1‑138 (MQ=255) ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTAtt > 1:829564/1‑139 (MQ=255) ctTGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGGTTGAGGGATGCATTCAGCGTAtt > 1:407296/1‑139 (MQ=255) ttGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTAt < 1:911867/137‑1 (MQ=255) tGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTTTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTcc > 1:634188/1‑139 (MQ=255) tGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTcc > 2:315144/1‑139 (MQ=255) tGTCCGTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGGGATGCATTCAGCGTATTcc > 1:1149814/1‑139 (MQ=255) gTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGt < 1:623925/139‑1 (MQ=255) gTCTTTCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGt < 1:1265559/139‑1 (MQ=255) ttCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTca > 2:952170/1‑137 (MQ=255) tCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcaca < 1:952170/138‑1 (MQ=255) tCTGGTGGGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTcaca < 2:94488/138‑1 (MQ=255) gtggGATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGc < 2:1261164/136‑1 (MQ=255) | TTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGACTGACAGCCGCCTCGACGGTTTGAGTGATGCATTCAGCGTATTCCGCTGTCACAGC > NZ_CP009273/753508‑753768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |