Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	59,627	C→A	intergenic (‑153/+60)	BW25113_RS25400 ← / ← rluA	transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	59,627	0	C	A	100.0%	303.5 / NA	75	intergenic (‑153/+60)	BW25113_RS25400/rluA	transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/47);  total (28/47)

AATATAAGAGACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTG  >  NZ_CP009273/59494‑59757                                                                                                                                      |                                                                                                                                   aaTATAAGAGACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCggg                                                                                                                               <  1:1022850/139‑1 (MQ=255) aaTATAAGAGACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCggg                                                                                                                               <  1:711931/139‑1 (MQ=255)     taAGAGACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGAt                                                                                                                            <  1:218806/138‑1 (MQ=255)           aCGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTa                                                                                                                     >  2:137610/1‑139 (MQ=255)           aCGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCACTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTa                                                                                                                     >  1:869218/1‑139 (MQ=255)             ggTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTAc                                                                                                                    >  1:1002326/1‑138 (MQ=255)             ggTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTAc                                                                                                                    >  1:25896/1‑138 (MQ=255)             ggTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTAc                                                                                                                    >  1:246022/1‑138 (MQ=255)             ggTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTAc                                                                                                                    <  2:25896/138‑1 (MQ=255)                  aCGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAgg                                                                                                              <  2:678983/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  1:616771/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  1:402139/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  1:840379/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  1:907466/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  2:367046/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  2:569332/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  2:999476/139‑1 (MQ=255)                   cGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  1:101532/139‑1 (MQ=255)                    gggTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCa                                                                                                            <  2:1002326/139‑1 (MQ=255)                     ggTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAAGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAg                                                                                                           <  2:1287542/139‑1 (MQ=255)                       tGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGcc                                                                                                         >  2:1010680/1‑139 (MQ=255)                         aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       <  2:1157655/139‑1 (MQ=255)                         aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       >  2:479928/1‑139 (MQ=255)                         aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       <  1:662576/139‑1 (MQ=255)                         aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       <  1:1010680/139‑1 (MQ=255)                         aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       <  1:1164020/139‑1 (MQ=255)                         aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       >  1:1157655/1‑139 (MQ=255)                         aagaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       >  1:424870/1‑139 (MQ=255)                           gaaAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCt                                                                                                       >  1:226641/1‑137 (MQ=255)                               aaGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGAt                                                                                                 <  2:956217/139‑1 (MQ=255)                               aaGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGAt                                                                                                 <  2:71680/139‑1 (MQ=255)                                 gTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGa                                                                                               <  2:1286362/139‑1 (MQ=255)                                         aTTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATaa                                                                                       <  2:272541/139‑1 (MQ=255)                                              cTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTa                                                                                  <  2:424870/139‑1 (MQ=255)                                                                aTTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  2:1266228/94‑1 (MQ=255)                                                                aTTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             >  1:1266228/1‑94 (MQ=255)                                                                   aCCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGAttt                                                                                                                          <  2:757109/78‑1 (MQ=255)                                                                   aCCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGAttt                                                                                                                          >  1:757109/1‑78 (MQ=255)                                                                   aCCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGa                                                                                               >  2:652271/1‑105 (MQ=255)                                                                   aCCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGa                                                                                               <  1:652271/105‑1 (MQ=255)                                                                   aCCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGc                                                             >  2:36762/1‑139 (MQ=255)                                                                    ccGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCt                                                            >  2:1105617/1‑139 (MQ=255)                                                                       aaTGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTAc                                                                                                                    >  1:1160955/1‑80 (MQ=255)                                                                       aaTGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTAc                                                                                                                    <  2:1160955/80‑1 (MQ=255)                                                                       aaTGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAgg                                                                                                              <  2:555666/86‑1 (MQ=255)                                                                       aaTGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAgg                                                                                                              >  1:555666/1‑86 (MQ=255)                                                                           gAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCtt                                                     >  2:228320/1‑139 (MQ=255)                                                                            aaaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCttt                                                    <  1:471806/139‑1 (MQ=255)                                                                             aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTa                                                   <  1:479928/139‑1 (MQ=255)                                                                             aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTa                                                   <  2:1203103/139‑1 (MQ=255)                                                                             aaGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTa                                                   <  1:1105617/139‑1 (MQ=255)                                                                              aGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             >  2:328143/1‑80 (MQ=255)                                                                              aGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGc                                                                                                             <  1:328143/80‑1 (MQ=255)                                                                                  tAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGt                                              >  1:1097516/1‑139 (MQ=255)                                                                                  tAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGt                                              <  2:1220328/139‑1 (MQ=255)                                                                                  tAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGt                                              <  1:228320/139‑1 (MQ=255)                                                                                    gCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCa                                            <  1:36762/139‑1 (MQ=255)                                                                                     ccACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaaa                                                 <  2:821648/133‑1 (MQ=255)                                                                                     ccACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTaaa                                                 >  1:821648/1‑133 (MQ=255)                                                                                        cacGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATAc                                         >  2:468822/1‑138 (MQ=255)                                                                                        cacGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATAc                                         >  1:250320/1‑138 (MQ=255)                                                                                         acGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTa                                       <  2:246022/139‑1 (MQ=255)                                                                                         acGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTa                                       <  1:424901/139‑1 (MQ=255)                                                                                         acGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTa                                       <  1:468822/139‑1 (MQ=255)                                                                                          cGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAg                                                                                                           <  1:729417/70‑1 (MQ=255)                                                                                          cGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAg                                                                                                           >  2:729417/1‑70 (MQ=255)                                                                                               caacTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCa                                 >  1:763976/1‑139 (MQ=255)                                                                                                 acTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATa                               <  1:137610/139‑1 (MQ=255)                                                                                                       aGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCgg                         <  1:1165484/139‑1 (MQ=255)                                                                                                       aGCAGTTTGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCg                          >  1:1122908/1‑138 (MQ=255)                                                                                                            tttGGCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATggg                    >  1:867547/1‑139 (MQ=255)                                                                                                               ggCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTa                  <  2:869218/138‑1 (MQ=255)                                                                                                                gCAGAAACAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTaa                 <  2:867547/138‑1 (MQ=255)                                                                                                                      aCAAAAAATCCCCGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCaa          <  1:215834/139‑1 (MQ=255)                                                                                                                               ccccGGAATCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTg  >  1:1245430/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                   AATATAAGAGACGGTCAACGGGTGAAGAAAAAGTTCAAAAATTCGCTGTGGAGCAGGAAGGGAATTACCGAATGGAAAGCGTAGCCACACGCAACAACTGAAAGCAGTTTGGCAGAAACAAAAAATCCCCGGACTCGGGGATTTATGTACAAGAGGCAGCCCTTAGGATGAGGGTATAAACGTACAGGAAAGGTTAAAAATCCGCTGGCGCTTTAAACGTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTG  >  NZ_CP009273/59494‑59757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑