Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	783,406	T→C	G296G (GGA→GGG)	galM ←	galactose‑1‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	783,406	0	T	C	100.0%	187.0 / NA	54	G296G (GGA→GGG)	galM	galactose‑1‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/22);  total (32/22)

TCAGGCTGGAATACTCTTCGCCAGGACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTTCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGCC  >  NZ_CP009273/783279‑783541                                                                                                                                |                                                                                                                                        tCAGGCTGGAATACTCTTCGCCAGGACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACgg                                                                                                                              >  2:791616/1‑139 (MQ=255)         gAATACTTTTCGCCAGGACGCAGGAATCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCTGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTgccg                                                                                                                       <  2:1244230/138‑1 (MQ=255)           aTACTCTTCGCCAGGACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGccgccg                                                                                                                    <  1:857249/139‑1 (MQ=255)                ctTCGCCAGGACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAgg                                                                                                                 >  2:1113026/1‑137 (MQ=255)                   cGCCAGGACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGtt                                                                                                            >  2:110813/1‑139 (MQ=255)                    gCCAGGACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTg                                                                                                           >  1:787228/1‑139 (MQ=255)                         gACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCg                                                                                                        >  1:1037033/1‑137 (MQ=255)                         gACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCg                                                                                                        >  2:1226105/1‑137 (MQ=255)                          aCGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAg                                                                                                     <  2:542588/139‑1 (MQ=255)                                  aGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGa                                                                                                 >  1:10294/1‑135 (MQ=255)                                  aGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGa                                                                                                 <  2:10294/135‑1 (MQ=255)                                     aGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCag                                                                                          <  1:627189/139‑1 (MQ=255)                                               cGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCg                                                                                <  2:1059951/139‑1 (MQ=255)                                               cGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCg                                                                                <  2:787228/139‑1 (MQ=255)                                                ggCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCgg                                                                               <  1:269821/139‑1 (MQ=255)                                                 gCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCggt                                                                              >  1:457852/1‑139 (MQ=255)                                                 gCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCggt                                                                              >  1:953815/1‑139 (MQ=255)                                                 gCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCggt                                                                              >  2:1288319/1‑139 (MQ=255)                                                 gCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCggt                                                                              >  2:735076/1‑139 (MQ=255)                                                          ggtggtTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGAc                                                                     >  1:524497/1‑139 (MQ=255)                                                            tggtTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCt                                                                   >  1:1017476/1‑139 (MQ=255)                                                               ttCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCgg                                                                               <  2:313753/124‑1 (MQ=255)                                                               ttCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCgg                                                                               >  1:313753/1‑124 (MQ=255)                                                               ttCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCg                                                                                <  2:87042/123‑1 (MQ=255)                                                               ttCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCg                                                                                >  1:87042/1‑123 (MQ=255)                                                               ttCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCtt                                                                  >  1:633972/1‑137 (MQ=255)                                                               ttCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCtt                                                                  >  1:619717/1‑137 (MQ=255)                                                               ctCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCgg                                                                               >  2:905533/2‑124 (MQ=255)                                                               ctCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCgg                                                                               <  1:905533/123‑1 (MQ=255)                                                                   ggCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTg                                                            <  1:1288319/139‑1 (MQ=255)                                                                        tCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAAtt                                                       >  1:134873/1‑139 (MQ=255)                                                                        tCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGCGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAAtt                                                       >  2:463646/1‑139 (MQ=255)                                                                               gAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCAt                                                 <  1:1113026/138‑1 (MQ=255)                                                                                aaaCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATct                                               <  1:735076/139‑1 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGa                                                                                                                                 >  2:609112/1‑55 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGa                                                                                                                                 >  1:34731/1‑55 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGa                                                                                                                                 <  2:34731/55‑1 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGa                                                                                                                                 <  1:609112/55‑1 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTg                                                                                             <  2:878252/91‑1 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTg                                                                                             >  1:878252/1‑91 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATctgc                                             >  1:992245/1‑139 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATctgc                                             >  1:1188600/1‑139 (MQ=255)                                                                                  aCTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATctgc                                             >  2:150486/1‑139 (MQ=255)                                                                                      gCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGAc                                         <  1:1226105/139‑1 (MQ=255)                                                                                       cTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCg                                                                                >  2:1024248/1‑99 (MQ=255)                                                                                       cTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCg                                                                                <  1:1024248/99‑1 (MQ=255)                                                                                       cTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGAc                                         >  2:1193918/1‑138 (MQ=255)                                                                                       cTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGAc                                         >  1:49002/1‑138 (MQ=255)                                                                                                 ccagccCTTGCCCGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATgcgc                              >  1:875081/1‑139 (MQ=255)                                                                                                    gccCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCgg                                                                               >  2:460458/1‑87 (MQ=255)                                                                                                    gccCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCgg                                                                               <  1:460458/87‑1 (MQ=255)                                                                                                    gccCTTGCCAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGcgccgc                           >  2:480690/1‑139 (MQ=255)                                                                                                           ccAGTCGGCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGCCCAGACATGCGCCGCCACTTTc                    <  2:909412/139‑1 (MQ=255)                                                                                                                  gCGTAAGGTTCGGTCCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCAt             <  1:791616/139‑1 (MQ=255)                                                                                                                            cggtccCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGc   >  1:1020267/1‑139 (MQ=255)                                                                                                                             ggtccCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGTCCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGcc  <  2:875081/139‑1 (MQ=255)                                                                                                                             ggtccCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGcc  <  2:353053/139‑1 (MQ=255)                                                                                                                             ggtccCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGcc  >  2:1041091/1‑139 (MQ=255)                                                                                                                             ggtccCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGcc  <  1:1272534/139‑1 (MQ=255)                                                                                                                             ggtccCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGcc  <  2:99106/139‑1 (MQ=255)                                                                                                                             ggtccCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGc   <  1:343240/138‑1 (MQ=255)                                                                                                                                |                                                                                                                                        TCAGGCTGGAATACTCTTCGCCAGGACGCAGGAAGCAGTCCGGTTGCGGCCATTCAGGGTGGTTCGGGCTGTCCGGTAGAAACTCGCTTTCCAGAGCCAGCCCTTGCCAGTCGGCGTAAGGTTCGGTTCCCCGCGACGGTGTGCCGCCGAGGAAGTTGCCGGAGTAGAATTGCAGAGCCGGAGCGGTGGTGTAGACCTTCAGCTGCAATTTTTCATCTGCTGACCAGACATGCGCCGCCACTTTCTTGCCATCGCCTTTGGCC  >  NZ_CP009273/783279‑783541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑