Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	797,386	T→C	I15T (ATC→ACC)	ybhI →	anion permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	797,386	0	T	C	100.0%	149.3 / NA	42	I15T (ATC→ACC)	ybhI	anion permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

AAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGT  >  NZ_CP009273/797251‑797514                                                                                                                                        |                                                                                                                                 aaGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               <  2:1284603/139‑1 (MQ=255) aaGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               <  2:764334/139‑1 (MQ=255)   gTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCAt                                                                                                                             <  1:94715/139‑1 (MQ=255)       aTCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGta                                                                                                                          <  2:604762/138‑1 (MQ=255)           tCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTattatt                                                                                                                     >  1:616100/1‑139 (MQ=255)           tCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTattatt                                                                                                                     >  1:288623/1‑139 (MQ=255)                      aTTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGcc                                                                                                          <  1:1018961/139‑1 (MQ=255)                          gTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGc                                                                                                       <  1:687568/138‑1 (MQ=255)                              tCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCgg                                                                                                  <  1:1233774/139‑1 (MQ=255)                                       ggACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTaa                                                                                         <  1:1043146/139‑1 (MQ=255)                                        gACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAg                                                                                        >  1:781183/1‑139 (MQ=255)                                        gACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAg                                                                                        >  1:1023336/1‑139 (MQ=255)                                            atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa                                                                                    >  2:449726/1‑139 (MQ=255)                                            atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa                                                                                    <  2:781183/139‑1 (MQ=255)                                            atcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGaa                                                                                    >  1:1212964/1‑139 (MQ=255)                                             tcaatAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAAc                                                                                   >  1:709379/1‑139 (MQ=255)                                                         ttCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGc                                                                       >  1:177901/1‑139 (MQ=255)                                                              tATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCttt                                                                    <  1:747089/137‑1 (MQ=255)                                                                   gTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGtattat                                                                                                                      <  1:955738/82‑1 (MQ=255)                                                                   gTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGtattat                                                                                                                      >  2:955738/1‑82 (MQ=255)                                                                      aTACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGttt                                                                                                                >  2:291524/1‑85 (MQ=255)                                                                      aTACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGttt                                                                                                                <  1:291524/85‑1 (MQ=255)                                                                      aTACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTa                                                          >  2:1277817/1‑139 (MQ=255)                                                                            aaagaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggc                                                    >  1:646983/1‑139 (MQ=255)                                                                              agaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               >  1:378262/1‑62 (MQ=255)                                                                              agaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGAcccc                                                                                                                               <  2:378262/62‑1 (MQ=255)                                                                               gaaCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 <  2:709379/139‑1 (MQ=255)                                                                               gaaCAGGATTTTAAACGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 <  1:318928/139‑1 (MQ=255)                                                                                 aCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 >  1:847590/1‑137 (MQ=255)                                                                                 aCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcggc                                                 <  2:847590/137‑1 (MQ=255)                                                                                 aCAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCa                                               >  2:451282/1‑139 (MQ=255)                                                                                           aaaTGAATAAGAAATCGTTATGGAAGCTAAGTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGc                                      <  2:905467/138‑1 (MQ=255)                                                                                            aaTGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTg                                    <  2:898367/139‑1 (MQ=255)                                                                                               gAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGt                                  >  2:697101/1‑138 (MQ=255)                                                                                                          cGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCtttt                                                                   <  1:464154/94‑1 (MQ=255)                                                                                                          cGTTATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCtttt                                                                   >  2:464154/1‑94 (MQ=255)                                                                                                            ttATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTcc                    >  1:773804/1‑139 (MQ=255)                                                                                                            ttATGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTcc                    >  2:400283/1‑139 (MQ=255)                                                                                                               tGGAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCg                  >  2:1152137/1‑138 (MQ=255)                                                                                                                 gAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGa                <  2:1212964/138‑1 (MQ=255)                                                                                                                 gAAGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGa                <  2:1032005/138‑1 (MQ=255)                                                                                                                  aaGCTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAAc              <  2:1023336/139‑1 (MQ=255)                                                                                                                     cTAATTCTGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTg           <  2:254393/139‑1 (MQ=255)                                                                                                                            tGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcg                                                   >  2:1253560/1‑92 (MQ=255)                                                                                                                            tGATATTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTggcg                                                   <  1:1253560/92‑1 (MQ=255)                                                                                                                              atatTAGCGACCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGt  >  1:517163/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                 AAGTTTATCTTCCCTGAAAAAATTCGTTGTCAGGATAAGGACAATCAATAAAGGACTTCTGTATGAGTCATACAGAAAGAACAGGATTTTAAATGAATAAGAAATCGTTATGGAAGCTAATTCTGATATTAGCGATCCCATGTATTATTGGTTTTATGCCAGCTCCGGCAGGATTAAGCGAACTGGCGTGGGTGCTTTTTGGTATTTACCTGGCGGCCATTGTGGGGCTGGTTATCAAGCCTTTCCCGGAACCTGTCGTACTGT  >  NZ_CP009273/797251‑797514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑