Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 803,049 G→A A106V (GCA→GTA)  ybhB ← kinase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273803,0490GA96.0% 173.8 / ‑2.5 50A106V (GCA→GTA) ybhBkinase inhibitor
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (26/22);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GGCGCTTGCCAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGC  >  NZ_CP009273/802913‑803180
                                                                                                                                        |                                                                                                                                   
ggCGCTTGCCAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTacg                                                                                                                                   >  1:857560/1‑139 (MQ=255)
ggCGCTTGCCAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTacg                                                                                                                                   <  1:97363/139‑1 (MQ=255)
ggCGCTTGCCAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTacg                                                                                                                                   <  1:1194612/139‑1 (MQ=255)
ggCGCTTGCCAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTacg                                                                                                                                   <  1:4175/139‑1 (MQ=255)
ggCGCTTGCCAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTacg                                                                                                                                   <  1:33476/139‑1 (MQ=255)
         cAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTAc                                                                                                                          <  2:640579/139‑1 (MQ=255)
         cAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTAc                                                                                                                          <  1:671845/139‑1 (MQ=255)
                 ggAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGttt                                                                                                                   >  1:1172410/1‑138 (MQ=255)
                   aaaTGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTAc                                                                                                                <  1:1150312/139‑1 (MQ=255)
                        aaCGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAg                                                                                                           >  1:909116/1‑139 (MQ=255)
                        aaCGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAg                                                                                                           >  1:744049/1‑139 (MQ=255)
                          cGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGCACGGTAAAAATGGAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGATTTACCAAAggc                                                                                                         >  2:727977/1‑137 (MQ=255)
                          cGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTc                                                                                                         >  1:907368/1‑139 (MQ=255)
                          cGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTc                                                                                                         >  1:678406/1‑139 (MQ=255)
                          cGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTc                                                                                                         >  2:699805/1‑139 (MQ=255)
                             tAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTAccc                                                                                                                        >  2:126213/1‑121 (MQ=255)
                             tAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTAccc                                                                                                                        <  1:126213/121‑1 (MQ=255)
                                     aCCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCt                                                                                              >  2:775976/1‑139 (MQ=255)
                                      ccATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCaaa                                                                                                            >  2:1072748/1‑124 (MQ=255)
                                      ccATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCaaa                                                                                                            <  1:1072748/124‑1 (MQ=255)
                                                        cgtCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCAt                                                                           <  2:575808/137‑1 (MQ=255)
                                                                    cAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTaccagac                                                                >  2:1038455/1‑138 (MQ=255)
                                                                     aaTACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTaccagacc                                                               <  1:53668/138‑1 (MQ=255)
                                                                       tACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTaccagacca                                                              >  2:1055859/1‑137 (MQ=255)
                                                                         cGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATAGTACCCGGTTTTACCAAAGTCGGGACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGc                                                          >  1:787969/1‑139 (MQ=255)
                                                                           ttCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCa                                                        <  2:838676/139‑1 (MQ=255)
                                                                              tataTCCAGCGCGTGAACGGAAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGGTACGCCATCGTACCCGGTTTTACCAAAGTCGGTAAGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAAc                                                     >  2:389556/1‑139 (MQ=255)
                                                                               atatCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAAcc                                                    >  1:833141/1‑139 (MQ=255)
                                                                                  tCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCtt                                                 <  1:1045685/139‑1 (MQ=255)
                                                                                  tCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCt                                                  <  2:744049/138‑1 (MQ=255)
                                                                                    cAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTg                                                <  1:491743/138‑1 (MQ=255)
                                                                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGt                                                                                                          <  1:1071829/79‑1 (MQ=255)
                                                                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGt                                                                                                          >  2:1071829/1‑79 (MQ=255)
                                                                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCg                                              >  1:776930/1‑139 (MQ=255)
                                                                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCg                                              >  1:177417/1‑139 (MQ=255)
                                                                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATc                                                                                                                              >  1:238421/1‑57 (MQ=255)
                                                                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATc                                                                                                                              <  2:238421/57‑1 (MQ=255)
                                                                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTac                                                                     <  1:305850/114‑1 (MQ=255)
                                                                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTac                                                                     >  2:305850/1‑114 (MQ=255)
                                                                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCgg                                             >  2:283914/1‑138 (MQ=255)
                                                                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCt                                                                                <  2:166928/102‑1 (MQ=255)
                                                                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCt                                                                                >  1:166928/1‑102 (MQ=255)
                                                                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGt                                            >  2:982683/1‑138 (MQ=255)
                                                                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGt                                            >  2:669862/1‑138 (MQ=255)
                                                                                          gTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTaa                                          >  1:764735/1‑138 (MQ=255)
                                                                                                ggTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCgg                                   <  1:775976/139‑1 (MQ=255)
                                                                                                  tAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGt                                 <  2:764735/139‑1 (MQ=255)
                                                                                                            gCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCa                                                        <  1:894085/106‑1 (MQ=255)
                                                                                                            gCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCa                                                        >  2:894085/1‑106 (MQ=255)
                                                                                                            gCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCggg                        <  1:459782/138‑1 (MQ=255)
                                                                                                            gCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGt                       <  1:1055859/139‑1 (MQ=255)
                                                                                                            gCGATGAGTTTCGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGt                       <  1:1038455/139‑1 (MQ=255)
                                                                                                                       cGCCTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTaacaa             <  2:833141/138‑1 (MQ=255)
                                                                                                                          cTTTCGGCGGTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTAc         <  1:120161/139‑1 (MQ=255)
                                                                                                                                 cggTGCTACGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGGATCAGCGGGTAAGTTAACAACTACCCAGGGc  >  2:1104926/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GGCGCTTGCCAGAGAGTGGAAATGAACGTTAAACCCGACCATCGCGCCGCTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGC  >  NZ_CP009273/802913‑803180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑