Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	861,119	G→A	R234R (CGC→CGT)	moeA ←	molybdopterin molybdotransferase MoeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	861,119	0	G	A	100.0%	163.5 / NA	43	R234R (CGC→CGT)	moeA	molybdopterin molybdotransferase MoeA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/26);  total (17/26)

GTTTAATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTG  >  NZ_CP009273/860983‑861253                                                                                                                                         |                                                                                                                                       gTTTAATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGgcacg                                                                                                                                      >  2:1168703/1‑139 (MQ=255) gTTTAATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGgcacg                                                                                                                                      >  1:362994/1‑139 (MQ=255) gTTTAATCGCCAGCTTCCAGAAGGCGACCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGgcacg                                                                                                                                      <  2:190551/139‑1 (MQ=255) gTTTAATCGCCAGCGTCCAGAAGGCGATCTCCCCCAGCCCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGgcacg                                                                                                                                      <  2:933624/139‑1 (MQ=255)  tttAATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGgcacgc                                                                                                                                     <  2:1221665/139‑1 (MQ=255)    tAATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCa                                                                                                                                    <  1:872730/138‑1 (MQ=255)    tAATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCa                                                                                                                                    <  2:723487/138‑1 (MQ=255)                        gCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGa                                                                                                               <  2:500503/139‑1 (MQ=255)                        gCGATCGCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGa                                                                                                               <  2:736032/139‑1 (MQ=255)                         cGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGAt                                                                                                              <  2:838441/139‑1 (MQ=255)                         cGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGAt                                                                                                              <  2:1099563/139‑1 (MQ=255)                                ccccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATcc                                                                                                         <  2:441423/137‑1 (MQ=255)                                ccccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCt                                                                                                       <  1:416545/139‑1 (MQ=255)                                  ccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTaa                                                                                                     <  1:854692/139‑1 (MQ=255)                                         ttCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTaa                                                                                                <  1:456904/137‑1 (MQ=255)                                           cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAAtt                                                                                              <  1:377745/137‑1 (MQ=255)                                           cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAAt                                                                                               <  1:236266/136‑1 (MQ=255)                                               aaTCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCg                                                                                        <  1:1211628/139‑1 (MQ=255)                                                 tCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCt                                                                                                       >  2:890795/1‑122 (MQ=255)                                                 tCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCt                                                                                                       <  1:890795/122‑1 (MQ=255)                                                   gTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATc                                                                                    >  1:1037375/1‑139 (MQ=255)                                                   gTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATc                                                                                    >  2:885690/1‑139 (MQ=255)                                                       tGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCa                                                                                 <  1:885690/138‑1 (MQ=255)                                                        ggTGTAATCCGCCTCGCCCACTGAAACACCGCCGGAGCTGATCACCACATCCGCCTGGCTGTCGTCTTCAATAAATGCGGCACGCAGGGCATGCGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCaa                                                                                <  2:1136803/138‑1 (MQ=255)                                                              aTCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTc                                                                          >  1:829673/1‑138 (MQ=255)                                                              aTCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCt                                                                         <  1:87230/139‑1 (MQ=255)                                                                   ccTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACAt                                                                    >  1:822476/1‑139 (MQ=255)                                                                    cTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACAt                                                                    >  1:979337/1‑138 (MQ=255)                                                                    cTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATc                                                                   <  1:316367/139‑1 (MQ=255)                                                                                      gccgGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACgg                                                 >  2:173036/1‑139 (MQ=255)                                                                                       ccgGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAg                                                                                                                                   >  2:736010/1‑56 (MQ=255)                                                                                       ccgGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAg                                                                                                                                   <  1:736010/56‑1 (MQ=255)                                                                                       ccgGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGt                                                >  1:271299/1‑139 (MQ=255)                                                                                         ggAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTg                                              <  1:1168703/139‑1 (MQ=255)                                                                                               gATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCg                                        >  1:541071/1‑139 (MQ=255)                                                                                                aTCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCg                                        >  2:605232/1‑138 (MQ=255)                                                                                                          ccGCCTGGCTGTCGGCTTCAATAAATGCGgcacgc                                                                                                                                     <  1:175389/35‑1 (MQ=255)                                                                                                          ccGCCTGGCTGTCGGCTTCAATAAATGCGgcacgc                                                                                                                                     >  2:175389/1‑35 (MQ=255)                                                                                                            gCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGt                           <  2:829673/139‑1 (MQ=255)                                                                                                            gCCTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGt                           <  2:822476/139‑1 (MQ=255)                                                                                                             ccTGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTc                          >  2:120729/1‑139 (MQ=255)                                                                                                               tGGCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGCGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGc                        >  2:88171/1‑139 (MQ=255)                                                                                                                 gCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGcc                       <  1:496907/138‑1 (MQ=255)                                                                                                                 gCTGTCGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGcc                       <  2:189815/138‑1 (MQ=255)                                                                                                                      cGGCTTCAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCgg                 >  1:996942/1‑139 (MQ=255)                                                                                                                            cAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATccc                                                                                  <  2:343463/68‑1 (MQ=255)                                                                                                                            cAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATccc                                                                                  >  1:343463/1‑68 (MQ=255)                                                                                                                            cAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGAcc           >  1:573137/1‑139 (MQ=255)                                                                                                                            cAATAAATGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGAc            <  2:362994/138‑1 (MQ=255)                                                                                                                                   tGCGGCACGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAAc    >  2:1002877/1‑139 (MQ=255)                                                                                                                                       gcgcgcAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTg  >  2:218747/1‑137 (MQ=255)                                                                                                                                         |                                                                                                                                       GTTTAATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATAAATGCGGCGCGCAGGGCATGGGGATCGTCGCGGATAATCCCTAAGTTAATTACCTCGCATCCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTG  >  NZ_CP009273/860983‑861253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑