Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	869,360	T→C	F309S (TTC→TCC)	pdeI →	EAL domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	869,360	0	T	C	100.0%	80.0 / NA	23	F309S (TTC→TCC)	pdeI	EAL domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/9);  total (14/9)

CAACGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTTCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAA  >  NZ_CP009273/869226‑869466                                                                                                                                       |                                                                                                           caacGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGt                                                                                                        >  2:117768/1‑139 (MQ=255)   acGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCg                                                                                                      >  1:688646/1‑139 (MQ=255)    cGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCgg                                                                                                     >  2:587761/1‑139 (MQ=255)         tattCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAAtt                                                                                                >  2:18925/1‑139 (MQ=255)               aaaTCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGa                                                                                          <  2:288726/139‑1 (MQ=255)                      tttttATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGa                                                                                     <  2:438114/137‑1 (MQ=255)                        tttATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACag                                                                                  <  1:587761/138‑1 (MQ=255)                          tattatCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACagag                                                                                <  2:596826/138‑1 (MQ=255)                            ttatCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTg                                                                             >  1:504989/1‑139 (MQ=255)                                    gCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATAc                                                                     >  1:716979/1‑139 (MQ=255)                                     cGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACg                                                                    <  1:280094/139‑1 (MQ=255)                                          aTTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGc                                                               >  2:75123/1‑139 (MQ=255)                                                            aTAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAAc                                             >  1:1259967/1‑139 (MQ=255)                                                                 cTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGAt                                         >  2:1028261/1‑138 (MQ=255)                                                                 cTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGAt                                         >  1:98548/1‑138 (MQ=255)                                                                                   gCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGaaa                       <  2:688646/138‑1 (MQ=255)                                                                                                   tATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGc      >  2:848557/1‑139 (MQ=255)                                                                                                     ttGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCg     >  1:966974/1‑138 (MQ=255)                                                                                                     ttGCTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGa    <  2:716979/139‑1 (MQ=255)                                                                                                     ttGCTGGTGGTTTATTTAATGACCACTCGTGTGTCACGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCg     >  2:667214/1‑138 (MQ=255)                                                                                                        cTGGTGGTTTATTTAATGACCACTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGaaa  <  1:667214/138‑1 (MQ=255)                                                                                                                              cTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACagag                                                                                >  1:897434/1‑38 (MQ=255)                                                                                                                              cTCGTGTGTCCCGTCGGCAATTTTCTGAAATGACagag                                                                                <  2:897434/38‑1 (MQ=255)                                                                                                                                       |                                                                                                           CAACGCATTATTCGAAATCTATTTTTATTATCCTGGCGGTAATTTATTGAACGCATCAGATAAACTTTTTTATCTGCCGTTTGCGTTCATTATTATCGTATTGCTGGTGGTTTATTTAATGACCACTCGTGTGTTCCGTCGGCAATTTTCTGAAATGACAGAGCTGGTTAATACGCTGGCGTTTTTGCCTGACTCAACGGATCAAATCGAGGCTCTGAAAATTCGTGAAGGCGATGCGAAA  >  NZ_CP009273/869226‑869466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑