| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 937,543 | A→C | T377P (ACC→CCC) | dmsA → | dimethylsulfoxide reductase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,543 | 0 | A | C | 88.0% | 149.7 / 13.2 | 50 | T377P (ACC→CCC) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (16/28); total (19/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01 | |||||||||||
CGCCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTG > NZ_CP009273/937406‑937678 | cgcCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTAc > 2:1257780/1‑139 (MQ=255)cgcCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTAc < 2:1204563/139‑1 (MQ=255) aTGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGc < 2:1176465/139‑1 (MQ=255) cACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTa < 1:1283840/139‑1 (MQ=255) taAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGc < 1:1192519/139‑1 (MQ=255) taAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGc < 1:1257780/139‑1 (MQ=255) aaGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCa < 1:1079506/139‑1 (MQ=255) aaGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCa < 1:385507/139‑1 (MQ=255) atatTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATg > 2:403465/1‑139 (MQ=255) gggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg > 1:665540/1‑139 (MQ=255) gggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCAAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg > 2:1129614/1‑139 (MQ=255) ggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg > 2:33404/1‑138 (MQ=255) ggTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATAAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCg > 2:1061658/1‑138 (MQ=255) aaGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCg > 2:372872/1‑139 (MQ=255) aGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTAt > 1:590797/1‑114 (MQ=255) aGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTAt < 2:590797/114‑1 (MQ=255) ccAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACg < 2:322515/139‑1 (MQ=255) ccAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACg < 2:915268/139‑1 (MQ=255) ccAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACg < 1:722651/139‑1 (MQ=255) cAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGc > 2:414320/1‑139 (MQ=255) cGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGt > 1:988736/1‑139 (MQ=255) aaaCGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGc < 2:525831/139‑1 (MQ=255) cGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCg < 2:201986/139‑1 (MQ=255) cGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCg > 2:133886/1‑139 (MQ=255) ggAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGt > 2:512027/1‑139 (MQ=255) gAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTg < 2:307002/139‑1 (MQ=255) gCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTAtct < 2:143567/139‑1 (MQ=255) cGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGAt > 2:496185/1‑139 (MQ=255) aTCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTgg < 1:237048/139‑1 (MQ=255) ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCTAAAc < 2:273645/51‑1 (MQ=255) ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAAc > 1:273645/1‑51 (MQ=255) ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAAc < 2:297451/109‑1 (MQ=255) ttCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAAc > 1:297451/1‑109 (MQ=255) ccGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCa < 2:782986/61‑1 (MQ=255) ccGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCa > 1:782986/1‑61 (MQ=255) ggCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGt < 2:665540/139‑1 (MQ=255) gCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGCCCGGt > 2:1144519/1‑138 (MQ=255) ggcAAAATCATCAAAGTGGCTCGTGATGTCGGTAGTCCCAAACCGGCGTTTATCAGCCAGTGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCGGGCTATTCTGACCTGTAACg < 2:414139/137‑1 (MQ=255) aCAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGt < 1:401868/139‑1 (MQ=255) cAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCTGTAACGtt < 2:176681/139‑1 (MQ=255) cAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGtt < 2:770493/139‑1 (MQ=255) cAAAATCATCAAATTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGtt < 1:634580/139‑1 (MQ=255) aaaTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACg < 2:222685/139‑1 (MQ=255) aaTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGc > 1:1087313/1‑44 (MQ=255) aaTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGc < 2:1087313/44‑1 (MQ=255) aTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGa < 1:512027/139‑1 (MQ=255) aTTGGCTCGTGAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGa < 1:861551/139‑1 (MQ=255) gcaaTCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTg > 1:302090/3‑67 (MQ=255) gAAATCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTg < 2:302090/67‑1 (MQ=255) aaTCGGTAGTCCCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAgcggc < 1:1144519/139‑1 (MQ=255) agtcCCAAACCGGCGGTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTg < 2:490995/139‑1 (MQ=255) | CGCCGAAAAATGGCCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTG > NZ_CP009273/937406‑937678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |