| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 62,451 | C→G | A272P (GCC→CCC) | rapA ← | RNA polymerase‑associated protein RapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 62,451 | 0 | C | G | 98.1% | 188.5 / ‑2.8 | 53 | A272P (GCC→CCC) | rapA | RNA polymerase‑associated protein RapA |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (29/23); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
TCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCTCATCATCAAATAGC > NZ_CP009273/62315‑62581 | tCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGtt > 2:751712/1‑139 (MQ=255) gggTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTACATCGACCACCAGCAGGTCCCATTCGGGTTca > 2:784534/1‑137 (MQ=255) gTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTcaca > 2:704981/1‑137 (MQ=255) gcgGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGAt > 1:874256/1‑139 (MQ=255) gcgGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGAt > 2:498774/1‑139 (MQ=255) gcagcagAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAg < 1:1017692/139‑1 (MQ=255) gcagAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGgcg < 2:924530/139‑1 (MQ=255) gcagAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGgcg < 2:827098/139‑1 (MQ=255) agAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCt < 2:706436/139‑1 (MQ=255) aaCGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGt > 1:178289/1‑139 (MQ=255) cGCCCGGCACGTGCTCTGCCAGTTGTTCGATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGttt > 1:54604/1‑139 (MQ=255) cccGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGc > 1:1223801/1‑139 (MQ=255) ggCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTac > 2:930338/1‑139 (MQ=255) gCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAAt > 2:120531/1‑139 (MQ=255) aGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCa < 2:54604/139‑1 (MQ=255) ttgttCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGc > 1:105129/1‑139 (MQ=255) cAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGc < 1:487680/138‑1 (MQ=255) aaTGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCa > 2:374859/1‑138 (MQ=255) aaTGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCa < 1:704981/138‑1 (MQ=255) ccTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCAc > 1:623230/1‑139 (MQ=255) atatTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAg < 2:178289/138‑1 (MQ=255) cACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTc > 2:1211114/1‑139 (MQ=255) ggCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGTCGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCa > 2:1203363/1‑138 (MQ=255) ggCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCa > 2:741679/1‑138 (MQ=255) gcgcATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCaaa < 1:805650/139‑1 (MQ=255) gcgcATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCaaa < 1:546924/139‑1 (MQ=255) cgcATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAAc < 2:1258223/139‑1 (MQ=255) cacaCCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGTCGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCa < 1:1203363/138‑1 (MQ=255) cAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCa > 1:394530/1‑133 (MQ=255) cAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCa < 2:394530/133‑1 (MQ=255) gTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCaaa > 2:161496/1‑117 (MQ=255) gTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCaaa < 1:161496/117‑1 (MQ=255) gTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCt > 1:547597/1‑137 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGCGCAAATCACCAGCTGTTCGGTGTCAACCGGGTTGTAAGCATCGTGCTGAGCtt > 1:441671/1‑139 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAg > 1:1144301/1‑98 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAg < 2:1144301/98‑1 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCtt > 2:296184/1‑139 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCtt < 1:751712/139‑1 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCtt > 2:67694/1‑139 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCtt > 1:957083/1‑139 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCtt < 1:296184/139‑1 (MQ=255) tGCGCTTCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGGGGTGAgatg > 2:1053696/1‑136 (MQ=255) tCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCg < 2:871174/99‑1 (MQ=255) tCATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCg > 1:871174/1‑99 (MQ=255) cATCGACCACCAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCa < 1:484233/137‑1 (MQ=255) accaccAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCt < 1:741679/139‑1 (MQ=255) accaccAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCt < 1:930338/139‑1 (MQ=255) accaccAGCAGGTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCt < 2:239141/139‑1 (MQ=255) gTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCt < 1:55659/128‑1 (MQ=255) gTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCt > 2:55659/1‑128 (MQ=255) gTCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCTCATCATCaaa > 2:518951/1‑138 (MQ=255) tCCCATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCTCATCATCaaa > 2:804277/1‑137 (MQ=255) ccATTCGGGTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCTCATCATCAAATAgc > 2:508634/1‑139 (MQ=255) | TCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCAAAATCCAGCGAGCAAATCACCAGCTGTTCGGTGTCAAACGGGTTGTAAGCATCGTGCTGAGCTTCGGCATAACGCTCATCATCAAATAGC > NZ_CP009273/62315‑62581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |