| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 980,640 | G→A | L176L (CTG→TTG) | aspC ← | aspartate transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 980,640 | 0 | G | A | 95.0% | 135.9 / ‑0.8 | 40 | L176L (CTG→TTG) | aspC | aspartate transaminase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (16/22); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01 | |||||||||||
CGGGCAAAACCCTGGTAAGCGAAGTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGCTCTTATGGTTCGG > NZ_CP009273/980510‑980775 | cGGGCAAAACCCTGGTAAGCGAAGTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAGCACTACGt > 1:656315/1‑139 (MQ=255) ggCAAAACCCTGGTAAGCGAAGTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCg < 2:370890/139‑1 (MQ=255) cccTGGTAAGCGAAGTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCt < 2:729101/139‑1 (MQ=255) aaGTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCttcattca < 2:683344/139‑1 (MQ=255) aaGTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCttcattca < 1:676114/139‑1 (MQ=255) gTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAgg < 2:366845/139‑1 (MQ=255) gTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAgg < 2:907640/139‑1 (MQ=255) gTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCttcattca > 2:497297/1‑126 (MQ=255) gTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCttcattca < 1:497297/126‑1 (MQ=255) gTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAg < 2:1076220/139‑1 (MQ=255) aGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATc < 2:662278/139‑1 (MQ=255) cGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAgt > 2:956762/1‑139 (MQ=255) tgCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTcc < 1:907361/139‑1 (MQ=255) ccAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGc < 1:425111/139‑1 (MQ=255) cAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGca < 2:294982/139‑1 (MQ=255) aGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGcat < 2:656315/139‑1 (MQ=255) tGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTTATCTGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCataa > 2:492596/1‑138 (MQ=255) gggTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACtt > 2:540404/1‑139 (MQ=255) tCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTc > 2:51257/1‑137 (MQ=255) tCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCa < 2:370621/139‑1 (MQ=255) aTACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTcc < 1:557732/105‑1 (MQ=255) aTACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTcc > 2:557732/1‑105 (MQ=255) aTACCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGAc > 1:814237/1‑139 (MQ=255) aCCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGc < 2:349441/44‑1 (MQ=255) aCCGGTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGc > 1:349441/1‑44 (MQ=255) ggTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTg > 2:976329/1‑137 (MQ=255) ggTTGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCa < 2:437471/139‑1 (MQ=255) ttGGGTTATGGCAGCAGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCa > 1:238571/1‑137 (MQ=255) gcagcaGGCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTAAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACg < 1:492596/139‑1 (MQ=255) gcagcaGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACg < 2:502727/139‑1 (MQ=255) gcagcaGCCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACg < 1:956762/139‑1 (MQ=255) gcagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGa > 2:113382/1‑79 (MQ=255) gcagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGa < 1:113382/79‑1 (MQ=255) gcagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGa < 1:979184/134‑1 (MQ=255) gcagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGa > 2:979184/1‑134 (MQ=255) gcagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGctc > 2:286088/1‑139 (MQ=255) gcagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGctc > 2:1012584/1‑139 (MQ=255) cagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCaa < 2:656076/70‑1 (MQ=255) cagcCATGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCaa > 1:656076/1‑70 (MQ=255) aTGGAACAACACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGCTCTTATgg > 2:1186713/1‑139 (MQ=255) acaacaCTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGCTCTTATGGTTCgg < 1:976329/139‑1 (MQ=255) | CGGGCAAAACCCTGGTAAGCGAAGTCAAACAGCGGTAACCAGCCTTTCTCAACGGAGAGTTGTGCCAGTGTTTGCCATTGTTCCAGCGTAGGGTCGATACCGGTTGGGTTATGGCAGCAGCCATGGAACAGCACTACGTCGCCAGCCTGAGCTTCATTCAGGCTGTTAATCAGTGCATCGAAGTCAAGAGTGTGATTTTCCGCATCATAATAAGCGTATTCACGAACTTCCAGACCTGCAGAGTTAAAGACGCTCTTATGGTTCGG > NZ_CP009273/980510‑980775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |