Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,006,907	T→C	D496D (GAT→GAC)	uup →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,006,907	0	T	C	100.0%	230.5 / NA	64	D496D (GAT→GAC)	uup	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/33);  total (31/33)

GCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGATCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCT  >  NZ_CP009273/1006773‑1007036                                                                                                                                       |                                                                                                                                  gcgcGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTc                                                                                                                               <  2:729657/139‑1 (MQ=255)    cgTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGt                                                                                                                            <  2:369833/139‑1 (MQ=255)      tttGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGttt                                                                                                                          <  2:108697/139‑1 (MQ=255)      tttGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGttt                                                                                                                          <  1:242539/139‑1 (MQ=255)             cTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGAt                                                                                                                    <  2:1150917/138‑1 (MQ=255)                   ccAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCg                                                                                                             <  2:1000475/139‑1 (MQ=255)                    cAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGt                                                                                                            >  2:1260707/1‑139 (MQ=255)                       gCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAaca                                                                                                                <  1:1098564/132‑1 (MQ=255)                       gCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAaca                                                                                                                >  2:1098564/1‑132 (MQ=255)                              aTTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGt                                                                                                  >  1:565873/1‑139 (MQ=255)                                tGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTg                                                                                                >  1:149334/1‑139 (MQ=255)                                     cTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCt                                                                                           >  2:376929/1‑139 (MQ=255)                                               cGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAggcgg                                                                                 <  1:1260707/139‑1 (MQ=255)                                               cGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAggcgg                                                                                 <  2:694096/139‑1 (MQ=255)                                                gACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACa                                                                                                        <  2:232963/115‑1 (MQ=255)                                                gACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACa                                                                                                        >  1:232963/1‑115 (MQ=255)                                                gACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAggcggc                                                                                >  1:742824/1‑139 (MQ=255)                                                   cAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGt                                                                             <  1:633573/139‑1 (MQ=255)                                                           ttGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGttt                                                                                                                          <  1:1016588/86‑1 (MQ=255)                                                           ttGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGttt                                                                                                                          >  2:1016588/1‑86 (MQ=255)                                                           ttGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTgg                                                                     >  1:711725/1‑139 (MQ=255)                                                            tGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGt                                                                    >  1:766324/1‑139 (MQ=255)                                                              aTGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCg                                                                  >  1:1139438/1‑139 (MQ=255)                                                                gTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGt                                                                 >  1:1153851/1‑138 (MQ=255)                                                                  cGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCg                                                                  <  1:556148/135‑1 (MQ=255)                                                                  cGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCg                                                                  >  2:556148/1‑135 (MQ=255)                                                                   gAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTgg                                                                                               >  1:262347/1‑105 (MQ=255)                                                                   gAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTgg                                                                                               <  2:262347/105‑1 (MQ=255)                                                                    aaaCGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGt                                                            <  1:1250984/139‑1 (MQ=255)                                                                     aaCGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTc                                                           <  1:376929/139‑1 (MQ=255)                                                                           ggAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGt                                                     >  1:922532/1‑139 (MQ=255)                                                                                     gaagaaCTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCatga                                             <  2:1013100/137‑1 (MQ=255)                                                                                         aaCTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCg                                       <  1:97192/139‑1 (MQ=255)                                                                                                 gACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGc                               <  1:197066/139‑1 (MQ=255)                                                                                                 gACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGc                               <  2:1139438/139‑1 (MQ=255)                                                                                                     gCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAaga                           <  1:1241375/139‑1 (MQ=255)                                                                                                     gCTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAaga                           <  2:711725/139‑1 (MQ=255)                                                                                                      cTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAagag                          <  1:1257624/139‑1 (MQ=255)                                                                                                      cTATCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAagag                          <  1:1168344/139‑1 (MQ=255)                                                                                                        aTCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAagag                          >  1:1140954/1‑137 (MQ=255)                                                                                                         tCAGGGCACGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAg                       >  1:160415/1‑139 (MQ=255)                                                                                                              gacaggTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTAtgt                  <  1:704773/135‑1 (MQ=255)                                                                                                                aCGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATaa                                                                                                                  >  2:390128/1‑41 (MQ=255)                                                                                                                aCGGTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATaa                                                                                                                  <  1:390128/41‑1 (MQ=255)                                                                                                                  ggTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTcgg                                                         >  2:969799/1‑96 (MQ=255)                                                                                                                  ggTATTGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTcgg                                                         <  1:969799/96‑1 (MQ=255)                                                                                                                       tGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGc                               <  2:1092110/117‑1 (MQ=255)                                                                                                                       tGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGc                               >  1:1092110/1‑117 (MQ=255)                                                                                                                       tGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCa          >  1:875756/1‑138 (MQ=255)                                                                                                                       tGCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCa          >  1:606472/1‑138 (MQ=255)                                                                                                                        gCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCaa         <  2:918522/138‑1 (MQ=255)                                                                                                                        gCTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCaa         <  2:922532/138‑1 (MQ=255)                                                                                                                         cTGGTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCaaa        <  1:997799/138‑1 (MQ=255)                                                                                                                            gTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCat                                               <  1:752388/96‑1 (MQ=255)                                                                                                                            gTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCat                                               >  2:752388/1‑96 (MQ=255)                                                                                                                            gTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGc    >  1:1226139/1‑139 (MQ=255)                                                                                                                            gTTAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGc    <  2:1140954/139‑1 (MQ=255)                                                                                                                              tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTTTCATGATGGCCGTGGGCAGCAAGAGGAGGATGTGGGGCCCCACCAGCCt  >  2:737471/1‑139 (MQ=255)                                                                                                                              tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTTTCATGATGGCCGCGGTTAGCAAGAGCCGTATGTGGCGCTCAAACAGCCt  >  1:511370/1‑139 (MQ=255)                                                                                                                              tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGGCCGGGGGCAGCAAAAGCAGGATGTGGCGCCCACACAGccc  >  2:791898/1‑138 (MQ=255)                                                                                                                              tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCCGTATGTGGCGCTCAAACAGCCt  >  1:941436/1‑139 (MQ=255)                                                                                                                              tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCt  >  1:892239/1‑139 (MQ=255)                                                                                                                              tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATAATGCCCGGGGGCAGCAAGAGCCGGATGTTGCGGTCAAAACGCCt  >  2:1262363/1‑139 (MQ=255)                                                                                                                              tAGCCACGACCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGGTTTCTTGATGCCCGTGGGCAGCAAGAGGAGGATGTGGGGGCCAAAcctgtc  >  2:704773/1‑134 (MQ=255)                                                                                                                                       |                                                                                                                                  GCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCGACCAACGATCTTGATGTCGAAACGCTGGAACTGCTGGAAGAACTGATCGACAGCTATCAGGGCACGGTATTGCTGGTTAGCCACGATCGTCAGTTTGTCGATAACACCGTTACAGAATGTTGGATCTTCGAAGGCGGCGGTAAAATTGGTCGTTATGTCGGCGGTTATCATGATGCCCGTGGTCAGCAAGAGCAGTATGTGGCGCTCAAACAGCCT  >  NZ_CP009273/1006773‑1007036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑