Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,017,704	C→A	A549S (GCC→TCC)	yccS ←	YccS family putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,017,704	0	C	A	100.0%	202.3 / NA	52	A549S (GCC→TCC)	yccS	YccS family putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/24);  total (28/24)

CCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCACCCAG  >  NZ_CP009273/1017578‑1017836                                                                                                                               |                                                                                                                                     ccGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCAt                                                                                                                          >  2:79624/1‑139 (MQ=255)                  tCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGa                                                                                                          >  1:848271/1‑138 (MQ=255)                          gtgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAg                                                                                                 >  1:1035920/1‑139 (MQ=255)                           tgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                <  2:1035920/139‑1 (MQ=255)                           tgtgCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                <  2:933970/139‑1 (MQ=255)                                aTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGc                                                                                           >  2:1074609/1‑139 (MQ=255)                                aTCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGc                                                                                           <  2:819420/139‑1 (MQ=255)                                 tCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCGGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          <  1:398277/139‑1 (MQ=255)                                  cgcgGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                >  2:945908/1‑132 (MQ=255)                                  cgcgGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                <  1:945908/132‑1 (MQ=255)                                  cgcgGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGc                                                                                           >  2:911078/1‑137 (MQ=255)                                    cggcggGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGc                                                                                           >  2:444650/1‑135 (MQ=255)                                     ggcggGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          <  1:319310/135‑1 (MQ=255)                                     ggcggGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGcc                                                                                          >  2:319310/1‑135 (MQ=255)                                                  atacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgcc                                                                         <  2:315192/139‑1 (MQ=255)                                                   tacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccg                                                                        >  1:1016407/1‑139 (MQ=255)                                                   tacgCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGGGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccg                                                                        >  1:308572/1‑139 (MQ=255)                                                           aCGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAg                                                                                                 <  2:723698/106‑1 (MQ=255)                                                           aCGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAg                                                                                                 >  1:723698/1‑106 (MQ=255)                                                           aCGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCa                                                                <  1:911078/139‑1 (MQ=255)                                                             ggTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAAt                                                              >  1:1043262/1‑139 (MQ=255)                                                                         ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgcc                                                                      >  2:542151/1‑119 (MQ=255)                                                                         ccTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTgccgcc                                                                      <  1:542151/119‑1 (MQ=255)                                                                             aTGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGc                                              >  2:962636/1‑139 (MQ=255)                                                                                 tATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTAt                                          <  1:962636/139‑1 (MQ=255)                                                                                  aTTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAg                                                                                        >  1:367799/1‑92 (MQ=255)                                                                                  aTTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAg                                                                                        <  2:367799/92‑1 (MQ=255)                                                                                           gTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCa                                                                    >  1:1245381/1‑103 (MQ=255)                                                                                           gTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCa                                                                    <  2:1245381/103‑1 (MQ=255)                                                                                           gTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATgg                                                            <  2:689701/111‑1 (MQ=255)                                                                                           gTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATgg                                                            >  1:689701/1‑111 (MQ=255)                                                                                                gCATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGt                            <  1:635290/138‑1 (MQ=255)                                                                                                 cATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                <  1:1026840/69‑1 (MQ=255)                                                                                                 cATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGt                                                                                                >  2:1026840/1‑69 (MQ=255)                                                                                                 cATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATg                                                                                     <  1:576635/80‑1 (MQ=255)                                                                                                 cATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATg                                                                                     >  2:576635/1‑80 (MQ=255)                                                                                                 cATCGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTaa                          >  1:691037/1‑139 (MQ=255)                                                                                                    cGAGATACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGc                       >  2:791955/1‑139 (MQ=255)                                                                                                     gagaTACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCt                      <  2:691037/139‑1 (MQ=255)                                                                                                      agaTACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTc                                                                                               <  2:35636/65‑1 (MQ=255)                                                                                                      agaTACCGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTc                                                                                               >  1:35636/1‑65 (MQ=255)                                                                                                            cGACAGTTGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCaa               <  1:735995/139‑1 (MQ=255)                                                                                                                  ttGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCtt         >  1:138663/1‑139 (MQ=255)                                                                                                                  ttGGCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCtt         >  1:1264465/1‑139 (MQ=255)                                                                                                                    ggCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATg                                                                                     <  1:713150/61‑1 (MQ=255)                                                                                                                    ggCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATg                                                                                     >  2:713150/1‑61 (MQ=255)                                                                                                                     gCCTCTGTGGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCAc      >  1:522339/1‑139 (MQ=255)                                                                                                                          tgtgGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                                  >  2:577221/1‑106 (MQ=255)                                                                                                                          tgtgGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                                  >  1:133387/1‑106 (MQ=255)                                                                                                                          tgtgGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                                  <  1:577221/106‑1 (MQ=255)                                                                                                                          tgtgGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTAcg                                  <  2:133387/106‑1 (MQ=255)                                                                                                                            tgGAGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCACCCAg  <  1:689130/136‑1 (MQ=255)                                                                                                                               |                                                                                                                                     CCGACGCCAGCTCAGCATCACGGTTGTGTGCATCGCGGCGGGCAATACGATACGCCAGACGGTTATCACGCCCCTGATGGTATTGCTCCAGTATGGCATCGAGATACCGACAGTTGGCCTCTGTGGCGCGTTCGAGCATGCGCGGCAGATTGCGAAACTGCCAGTCAGGCCAGATGTAGCTCACTGCCGCCCACGCAATGGCACAACCAATCAGCGTATCGATTACGCGAGGTAACGCTACTTCAAAACCTTCACCCAG  >  NZ_CP009273/1017578‑1017836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑