Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,043,563	A→C	G195G (GGT→GGG)	gfcC ←	capsule biosynthesis GfcC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,043,563	0	A	C	100.0%	177.8 / NA	49	G195G (GGT→GGG)	gfcC	capsule biosynthesis GfcC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/27);  total (22/27)

CAGACACAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCA  >  NZ_CP009273/1043427‑1043693                                                                                                                                         |                                                                                                                                   cAGACACAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTcccc                                                                                                                                  <  2:1215684/139‑1 (MQ=255)   gACACAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCt                                                                                                                                <  1:581152/139‑1 (MQ=255)   gACACAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCt                                                                                                                                <  2:419843/139‑1 (MQ=255)   gACACAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCt                                                                                                                                <  2:612650/139‑1 (MQ=255)   gACACAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCt                                                                                                                                >  2:752537/1‑139 (MQ=255)    acacAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCt                                                                                                                                >  1:572010/1‑138 (MQ=255)     cacaATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCa                                                                                                                              <  1:714723/139‑1 (MQ=255)              gTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTa                                                                                                                        <  2:69163/136‑1 (MQ=255)                  tttAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCa                                                                                                                 <  2:347743/139‑1 (MQ=255)                  tttAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCa                                                                                                                 >  2:1247306/1‑139 (MQ=255)                  tttAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCa                                                                                                                 >  2:538702/1‑139 (MQ=255)                       aTCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACg                                                                                                            <  2:515523/139‑1 (MQ=255)                                          aGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTg                                                                                         <  2:572010/139‑1 (MQ=255)                                                   gcAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCggg                                                                                >  2:463688/1‑139 (MQ=255)                                                      gAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCgg                                                                                 >  1:850756/1‑135 (MQ=255)                                                      gAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCgg                                                                                 <  2:850756/135‑1 (MQ=255)                                                      gAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGt                                                                             >  1:1042306/1‑139 (MQ=255)                                                      gAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGt                                                                             >  2:930629/1‑139 (MQ=255)                                                       aaaaGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTg                                                                            <  2:811434/139‑1 (MQ=255)                                                                               cAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCt                                                    <  1:201123/139‑1 (MQ=255)                                                                                        gtTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctg                                            <  2:283328/138‑1 (MQ=255)                                                                                                   cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAAt                                                              >  1:802169/1‑109 (MQ=255)                                                                                                   cTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAAt                                                              <  2:802169/109‑1 (MQ=255)                                                                                                    ttttATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATc                                                                         <  1:975998/97‑1 (MQ=255)                                                                                                    ttttATTCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATc                                                                         >  2:975998/1‑97 (MQ=255)                                                                                                         ttCCACAGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGcc                          <  1:1102125/139‑1 (MQ=255)                                                                                                             acaGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctg                                            >  2:583179/1‑117 (MQ=255)                                                                                                             acaGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGgcctg                                            <  1:583179/117‑1 (MQ=255)                                                                                                             acaGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGc                      >  1:598152/1‑139 (MQ=255)                                                                                                              caGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTa                                                         >  1:298680/1‑103 (MQ=255)                                                                                                              caGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTa                                                         <  2:298680/103‑1 (MQ=255)                                                                                                              caGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGcc                     >  1:5479/1‑139 (MQ=255)                                                                                                              caGGGCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGcc                     >  2:1246851/1‑139 (MQ=255)                                                                                                                 ggCAACAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGccc                    >  1:1275532/1‑137 (MQ=255)                                                                                                                      cAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                          >  1:623995/1‑94 (MQ=255)                                                                                                                      cAGGGGCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGt                                                          <  2:623995/94‑1 (MQ=255)                                                                                                                          ggCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCa         <  1:463688/139‑1 (MQ=255)                                                                                                                           gCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAc        >  2:1052237/1‑139 (MQ=255)                                                                                                                           gCGACGACAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAc        >  2:251284/1‑139 (MQ=255)                                                                                                                            cgacgaCAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTg                                                                                         <  2:490042/57‑1 (MQ=255)                                                                                                                            cgacgaCAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTg                                                                                         >  1:490042/1‑57 (MQ=255)                                                                                                                            cgacgaCAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAcc       <  1:251284/139‑1 (MQ=255)                                                                                                                             gacgacAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCAccc      <  1:845395/139‑1 (MQ=255)                                                                                                                               cgacAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCcag    <  2:773581/139‑1 (MQ=255)                                                                                                                               cgacAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCcag    <  2:278437/139‑1 (MQ=255)                                                                                                                                 acAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGcc                          <  1:256666/115‑1 (MQ=255)                                                                                                                                 acAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGcc                          >  2:256666/1‑115 (MQ=255)                                                                                                                                 acAGTCTCCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCcagca  <  2:598152/139‑1 (MQ=255)                                                                                                                                     tctcCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGaaa              <  2:348170/123‑1 (MQ=255)                                                                                                                                     tctcCCCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGaaa              >  1:348170/1‑123 (MQ=255)                                                                                                                                         |                                                                                                                                   CAGACACAATCTGGTCATTTAAATCAGCATATTTTTCGGGCAGTACATGCGCAGAAAAGCCCAACCAAAGCTGGCTGCCAGGCGGTGGTTCCACATGTCTTTTATTCCACAGGGCAACAGGGGCGACGACAGTCTCACCCTCAGGCGTAATCACCATAACGTTATTCTTGTCCGCGCCTGCAAGGCGGGGGTGATCTTGCAGATAATCGGTAACGCTACGGCCTGCCTGCCAGGGCAATTGCCCGGCCCCAGAAACCGCACCCAGCA  >  NZ_CP009273/1043427‑1043693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑