| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,077,887 | G→A | K63K (AAG→AAA) | efeO → | iron uptake system protein EfeO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,077,887 | 0 | G | A | 94.1% | 179.9 / ‑0.6 | 51 | K63K (AAG→AAA) | efeO | iron uptake system protein EfeO |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (20/28); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTC > NZ_CP009273/1077757‑1078011 | ctGCTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTg < 1:1139707/137‑1 (MQ=255)ctGCTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTgg < 1:79406/138‑1 (MQ=255)ctGCTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTgg < 1:1227733/138‑1 (MQ=255) cTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTACCGACGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGATGGAGTg > 1:1150876/1‑139 (MQ=255) cTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGa < 1:696141/136‑1 (MQ=255) cTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTg > 1:970085/1‑139 (MQ=255) aTGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGgag < 2:374206/138‑1 (MQ=255) aTGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGgag < 1:638390/138‑1 (MQ=255) aCGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTc < 1:505295/137‑1 (MQ=255) aCGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCaa < 1:1142521/139‑1 (MQ=255) gcTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCg < 1:461660/139‑1 (MQ=255) aTGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAt < 2:575252/139‑1 (MQ=255) aTGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAt < 1:1065322/139‑1 (MQ=255) tgCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGgag > 1:1150840/1‑119 (MQ=255) tgCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGgag < 2:1150840/119‑1 (MQ=255) ccGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAtggtgg > 2:108205/1‑139 (MQ=255) ggTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTg > 2:57303/1‑127 (MQ=255) ggTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTg < 1:57303/127‑1 (MQ=255) ggTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAtggtggtgg > 1:83905/1‑137 (MQ=255) aaaGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAagag < 2:699266/138‑1 (MQ=255) aaaGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAagag < 2:304558/138‑1 (MQ=255) ccGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACTCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGaaaa > 2:855519/1‑139 (MQ=255) ccGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCg < 2:769367/114‑1 (MQ=255) ccGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCg > 1:769367/1‑114 (MQ=255) aTGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGaaaa > 1:192923/1‑139 (MQ=255) tGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAAt > 2:683081/1‑139 (MQ=255) tGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAAt > 1:549771/1‑139 (MQ=255) aaCGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAatat > 2:474141/1‑101 (MQ=255) aaCGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAatat < 1:474141/101‑1 (MQ=255) aaCGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTa > 1:1702/1‑138 (MQ=255) gCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGc < 1:1289933/139‑1 (MQ=255) gCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGc < 2:192923/139‑1 (MQ=255) acacAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGgag > 1:710134/1‑48 (MQ=255) acacAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGgag < 2:710134/48‑1 (MQ=255) acacAGTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAAt < 2:549771/139‑1 (MQ=255) gTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGat < 1:855519/139‑1 (MQ=255) gTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGat < 2:1115625/139‑1 (MQ=255) gTTCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGa > 1:58592/1‑138 (MQ=255) ttCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAt > 1:536124/1‑59 (MQ=255) ttCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGAt < 2:536124/59‑1 (MQ=255) ttCATTATTCAGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGG‑AAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGatat < 1:701213/139‑1 (MQ=255) aGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGc > 1:1162271/1‑89 (MQ=255) aGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGc < 2:1162271/89‑1 (MQ=255) aGAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCg < 2:349315/138‑1 (MQ=255) gAACCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGt < 2:1251151/139‑1 (MQ=255) aaCCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGcccc > 2:633989/1‑77 (MQ=255) aaCCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGcccc < 1:633989/77‑1 (MQ=255) aaCCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGc > 1:1189046/1‑101 (MQ=255) aaCCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGc < 2:1189046/101‑1 (MQ=255) aaCCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTc > 2:1140584/1‑139 (MQ=255) aaCCACAGCCAGAAAGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTc > 1:66785/1‑139 (MQ=255) | CTGCTTTTATGGCTAACGCCGCTGATGTGCCGCAGGTCAAAGTGACCGTGACGGATAAGCAGTGCGAACCGATGACCATTACGGTTAACGCCGGGAAAACACAGTTCATTATTCAGAACCACAGCCAGAAGGCGCTGGAGTGGGAGATCCTCAAAGGCGTGATGGTGGTGGAAGAGCGGGAAAATATCGCCCCTGGCTTTAGCCAGAAAATGACGGCGAATTTACAGCCTGGCGAATACGATATGACCTGCGGTC > NZ_CP009273/1077757‑1078011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |