Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,097,827 A→C G57G (GGT→GGG)  csgE ← curli production assembly/transport protein CsgE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,097,8270AC100.0% 130.3 / NA 36G57G (GGT→GGG) csgEcurli production assembly/transport protein CsgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/18);  total (18/18)

TGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCG  >  NZ_CP009273/1097695‑1097953
                                                                                                                                    |                                                                                                                              
tGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGta                                                                                                                          >  2:748316/1‑139 (MQ=255)
    aaGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGt                                                                                                                      >  2:583894/1‑139 (MQ=255)
          acAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCa                                                                                                                    <  1:1233348/135‑1 (MQ=255)
          acAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCa                                                                                                                    >  2:1233348/1‑135 (MQ=255)
                 tctcGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCAtt                                                                                                          <  2:816338/138‑1 (MQ=255)
                     gAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACtt                                                                                                                 <  2:1149936/127‑1 (MQ=255)
                     gAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCa                                                                                                     >  2:351519/1‑139 (MQ=255)
                        gTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTa                                                                                                  <  2:1233245/139‑1 (MQ=255)
                                         aTAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATc                                                                                 <  1:661719/139‑1 (MQ=255)
                                                aGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCa                                                                          <  2:779569/139‑1 (MQ=255)
                                                           tAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGaaa                                                                >  2:121454/1‑138 (MQ=255)
                                                           tAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAAc                                                               <  1:618695/139‑1 (MQ=255)
                                                                  cTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATg                                                         <  1:748316/138‑1 (MQ=255)
                                                                                  gTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATAGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCg                                        <  2:576164/139‑1 (MQ=255)
                                                                                   tGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCgg                                       <  1:351519/139‑1 (MQ=255)
                                                                                     aTCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGt                                                       >  1:297069/1‑121 (MQ=255)
                                                                                     aTCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGt                                                       <  2:297069/121‑1 (MQ=255)
                                                                                       ccAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATg                                                                              >  2:1276901/1‑96 (MQ=255)
                                                                                       ccAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATg                                                                              <  1:1276901/96‑1 (MQ=255)
                                                                                        cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCggg                                      >  2:541185/1‑135 (MQ=255)
                                                                                        cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCggg                                      <  1:541185/135‑1 (MQ=255)
                                                                                        cAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCAATAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACtt                                  >  2:1256940/1‑139 (MQ=255)
                                                                                         aGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                 <  1:882821/139‑1 (MQ=255)
                                                                                          gCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTc                                 >  2:1053030/1‑138 (MQ=255)
                                                                                           cTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACt                                   >  1:1151481/1‑135 (MQ=255)
                                                                                           cTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACt                                   <  2:1151481/135‑1 (MQ=255)
                                                                                                ccATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCa                          <  1:583894/139‑1 (MQ=255)
                                                                                                             ggCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAggt             >  2:754855/1‑137 (MQ=255)
                                                                                                             ggCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAt             >  2:107661/1‑139 (MQ=255)
                                                                                                             ggCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAt             >  2:640105/1‑139 (MQ=255)
                                                                                                             ggCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAt             >  1:476825/1‑139 (MQ=255)
                                                                                                              gCCTTTCATTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGAtt            >  1:763360/1‑139 (MQ=255)
                                                                                                                     aTTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCAttt                                                                                                         <  2:368094/39‑1 (MQ=255)
                                                                                                                     aTTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCAttt                                                                                                         >  1:368094/1‑39 (MQ=255)
                                                                                                                     aTTAATCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTgcg     <  2:1166496/139‑1 (MQ=255)
                                                                                                                         aTCGTTAAGTTCCCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTgcggcg  >  1:144761/1‑138 (MQ=255)
                                                                                                                                    |                                                                                                                              
TGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCG  >  NZ_CP009273/1097695‑1097953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑