Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,100,664	A→T	D83V (GAT→GTT)	csgC →	curli assembly chaperone CsgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,100,664	0	A	T	100.0%	162.0 / NA	41	D83V (GAT→GTT)	csgC	curli assembly chaperone CsgC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/26);  total (15/26)

GCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAGAGAATGGT  >  NZ_CP009273/1100532‑1100792                                                                                                                                     |                                                                                                                                 gCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGt                                                                                                                            >  2:1288928/1‑139 (MQ=255)         cAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAg                                                                                                                    <  1:750712/139‑1 (MQ=255)          aaaTATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAgt                                                                                                                   >  1:348743/1‑139 (MQ=255)          aaaTATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAgt                                                                                                                   >  2:1000946/1‑139 (MQ=255)          aaaTATTGTCCCTGCGCGAAGGCAGTTCAGG‑‑‑AAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAgttgt                                                                                                                >  2:584440/1‑139 (MQ=255)               ttGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTa                                                                                                              <  2:898125/139‑1 (MQ=255)                 gTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACt                                                                                                            >  2:602695/1‑139 (MQ=255)                 gTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACt                                                                                                            >  2:400362/1‑139 (MQ=255)                    ccTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGtt                                                                                                         >  2:236619/1‑139 (MQ=255)                    ccTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGt                                                                                                          <  1:1000946/138‑1 (MQ=255)                           gAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATg                                                                                                  <  2:840859/139‑1 (MQ=255)                              ggCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGAc                                                                                               <  1:1073098/139‑1 (MQ=255)                                   ttCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCa                                                                                          >  1:1031707/1‑139 (MQ=255)                                        ggCAAAGTCAGGCGAAGCGAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGGTGCTGTTTCTGATGGACAGTCACTTCa                                                                                     <  1:507754/139‑1 (MQ=255)                                        ggCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCa                                                                                     <  1:1157150/139‑1 (MQ=255)                                        ggCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCa                                                                                     <  1:89281/139‑1 (MQ=255)                                             aGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTAt                                                                                >  2:1080933/1‑139 (MQ=255)                                             aGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTAt                                                                                <  2:255006/139‑1 (MQ=255)                                                  gACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCacaa                                                                           >  1:994680/1‑139 (MQ=255)                                                  gACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCacaa                                                                           <  1:236619/139‑1 (MQ=255)                                                        gCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATgg                                                                     <  1:481618/139‑1 (MQ=255)                                                             aaaaGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGgccgcc                                                                <  2:741407/139‑1 (MQ=255)                                                             aaaaGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGgccgcc                                                                <  2:348743/139‑1 (MQ=255)                                                             aaaaGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGgccgcc                                                                <  2:1031707/139‑1 (MQ=255)                                                                        tCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGa                                                       <  2:994680/137‑1 (MQ=255)                                                                            tGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTc                                                 >  1:873363/1‑139 (MQ=255)                                                                                  cTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAAtt                                           <  1:584440/139‑1 (MQ=255)                                                                                         aCCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGaa                                    <  1:344386/139‑1 (MQ=255)                                                                                               tGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACa                                                                                              <  2:44149/75‑1 (MQ=255)                                                                                               tGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACa                                                                                              >  1:44149/1‑75 (MQ=255)                                                                                                 cTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAg                            <  1:400362/139‑1 (MQ=255)                                                                                                    tGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATttg                                         <  1:288639/123‑1 (MQ=255)                                                                                                    tGACGAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATttg                                         >  2:288639/1‑123 (MQ=255)                                                                                                        gAAGTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGa                     <  1:1288928/139‑1 (MQ=255)                                                                                                           gTTGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATg                   >  2:1020764/1‑138 (MQ=255)                                                                                                            ttGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGgccgcc                                                                >  2:25454/1‑92 (MQ=255)                                                                                                            ttGAGTTTAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGgccgcc                                                                <  1:25454/92‑1 (MQ=255)                                                                                                                 tttAAATATTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGTACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTgg            <  1:112936/139‑1 (MQ=255)                                                                                                                      atatTTCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGgagaga       <  2:410510/139‑1 (MQ=255)                                                                                                                         tttCCCCGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAGAGAATg    <  2:873363/139‑1 (MQ=255)                                                                                                                            ccccGGACGTTCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTACCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAGAGAATGGt  <  2:1184850/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 GCAGAGTACAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAGAGAATGGT  >  NZ_CP009273/1100532‑1100792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑